GAS8 (NM_001286209) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CILD33; DRC4; GAS11
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336205 representing NM_001286209. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGAGCAAGGAGCAGGTGGAGGAGCATGTCAGCCGCATCCGGGAGGAGCTGGACCGCGAGCGGGAGGAA CGAAACTACTTCCAGCTGGAGCGGGACAAGATCCACACCTTCTGGGAGATCACACGGAGGCAGCTGGAG GAGAAGAAGGCTGAGCTGCGGAACAAAGACCGGGAGATGGAAGAAGCCGAGGAGAGGCACCAGGTGGAG ATCAAGGTGTACAAGCAGAAAGTGAAGCACCTGCTATATGAGCACCAGAACAACCTGACAGAGATGAAG GCTGAGGGCACTGTAGTCATGAAGCTGGCACAGAAAGAGCACCGCATACAGGAGAGTGTGCTGCGCAAG GACATGCGGGCACTGAAGGTGGAGCTCAAGGAGCAGGAGCTGGCCAGTGAGGTGGTGGTGAAGAACCTG CGGCTGAAACACACCGAGGAGATCACCAGGATGCGGAATGATTTTGAGAGGCAAGTTCGAGAAATTGAG GCCAAGTATGATAAGAAGATGAAGATGCTGAGGGACGAACTCGACTTGCGGAGAAAGACTGAGCTCCAC GAAGTGGAGGAGAGGAAGAATGGCCAGATCCACACGCTGATGCAGCGCCACGAGGAGGCCTTCACCGAC ATTAAGAACTACTACAACGACATCACCCTCAACAACCTGGCCCTCATCAACTCCCTCAAGGAGCAGATG GAGGACATGCGGAAGAAGGAGGACCACCTGGAGAGGGAGATGGCAGAGGTGTCTGGGCAGAACAAGCGC CTGGCAGACCCTCTCCAGAAGGCTCGGGAGGAGATGAGCGAGATGCAGAAACAGCTCGCAAACTACGAG AGGGACAAGCAGATCCTGCTTTGCACAAAAGCCCGTTTGAAAGTCAGGGAGAAAGAGCTGAAAGACCTG CAGTGGGAGCATGAAGTGTTAGAGCAGCGATTCACCAAGGTGCAGCAGGAGCGGGACGAGCTCTATCGG AAGTTCACCGCAGCCATCCAGGAGGTGCAGCAGAAGACAGGGTTCAAGAACCTCGTGCTAGAACGCAAG CTGCAGGCTCTGAGCGCCGCTGTGGAGAAGAAGGAGGTGCAGTTCAACGAGGTCCTGGCTGCCTCTAAC CTGGACCCTGCAGCCCTGACGCTGGTGTCCCGCAAGCTGGAGGATGTTCTTGAGTCGAAGAACAGCACC ATCAAGGACCTGCAGTATGAGCTGGCCCAGGTCTGTAAGGCCCATAACGACCTGCTGCGCACGTATGAG GCAAAGCTGCTGGCCTTCGGGATCCCTCTGGACAACGTGGGCTTCAAGCCCTTGGAAACAGCTGTGATC GGACAGACACTGGGCCAGGGCCCCGCGGGACTGGTGGGCACCCCGACGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001286209
Insert Size	1362 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001286209.1
RefSeq Size	3200 bp
RefSeq ORF	1362 bp
Locus ID	2622
UniProt ID	O95995
MW	53.8 kDa
Gene Summary	This gene includes 11 exons spanning 25 kb and maps to a region of chromosome 16 that is sometimes deleted in breast and prostrate cancer. The second intron contains an apparently intronless gene, C16orf3, that is transcribed in the opposite orientation. This gene is a putative tumor suppressor gene. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2013] Transcript Variant: This variant (5) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (d) is shorter at the N-terminus compared to isoform a.

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