CTP synthase (CTPS1) (NM_001301237) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CTPS; GATD5; GATD5A; IMD24
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336112 representing NM_001301237. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGCCCTTTATTGAGGCCTTCCGTCAGTTCCAATTCAAGGTCAAAAGAGAGAACTTTTGTAACATCCAC GTCAGTCTAGTTCCCCAGCCAAGTTCAACAGGGGAACAGAAGACTAAACCTACCCAGAATAGTGTTCGG GAACTTAGAGGACTTGGGCTTTCCCCAGATCTGGTTGTATGCAGGTGCTCAAATCCACTTGACACATCA GTGAAGGAGAAAATATCAATGTTCTGCCATGTTGAGCCTGAACAAGTGATCTGTGTCCACGATGTCTCA TCCATCTACCGAGTCCCCTTGTTGTTAGAGGAGCAAGGGGTTGTAGATTATTTTCTTCGAAGACTTGAC CTTCCTATTGAGAGGCAGCCAAGAAAAATGCTGATGAAATGGAAAGAGATGGCTGACAGATATGATCGC TTGCTGGAGACCTGCTCTATTGCCCTTGTGGGCAAATACACGAAGTTCTCAGACTCCTATGCCTCTGTC ATTAAGGCTCTGGAGCATTCTGCACTGGCCATCAACCACAAATTGGAAATCAAGTACATAGATTCTGCG GACTTGGAGCCCATCACCTCGCAAGAAGAGCCCGTGCGCTACCACGAAGCTTGGCAGAAGCTCTGTAGT GCTCATGGAGTGCTGGTTCCAGGAGGATTTGGTGTTCGAGGAACAGAAGGAAAAATCCAAGCAATTGCC TGGGCTCGGAATCAGAAAAAGCCTTTTTTGGGCGTGTGCTTAGGGATGCAGTTGGCAGTGGTTGAATTC TCAAGAAACGTGCTGGGATGGCAAGATGCCAATTCTACAGAGTTTGACCCTACGACCAGTCATCCCGTG GTCGTAGACATGCCAGAACACAACCCAGGGCAGATGGGCGGAACCATGAGGCTGGGCAAGAGGAGAACC CTGTTCCAGACCAAGAACTCAGTCATGAGGAAACTCTATGGAGACGCAGACTACTTGGAAGAGAGGCAC CGCCACCGATTTGAGGTGAATCCAGTCTGGAAAAAGTGTTTGGAAGAACAAGGCTTGAAGTTTGTTGGC CAAGATGTTGAAGGAGAGAGAATGGAAATTGTGGAGTTAGAAGATCATCCCTTTTTTGTTGGGGTTCAG TACCACCCTGAGTTCCTGTCCAGGCCTATCAAGCCCTCCCCACCATACTTTGGCCTCCTCCTGGCCTCT GTGGGGCGGCTCTCACATTACCTCCAGAAAGGCTGCAGGCTCTCACCCAGGGACACCTATAGTGACAGG AGTGGAAGCAGCTCCCCTGACTCTGAAATCACCGAACTGAAGTTTCCATCAATAAATCATGACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001301237
Insert Size	1308 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001301237.1
RefSeq Size	2412 bp
RefSeq ORF	1308 bp
Locus ID	1503
UniProt ID	P17812
Protein Pathways	Metabolic pathways, Pyrimidine metabolism
MW	49.7 kDa
Gene Summary	This gene encodes an enzyme responsible for the catalytic conversion of UTP (uridine triphosphate) to CTP (cytidine triphospate). This reaction is an important step in the biosynthesis of phospholipids and nucleic acids. Activity of this proten is important in the immune system, and loss of function of this gene has been associated with immunodeficiency. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014] Transcript Variant: This variant (2) differs in the 5' UTR, lacks multiple 5' coding exons, and initiates translation at a downstream in-frame start codon, compared to variant 1. The encoded isoform (b) has a shorter N-terminus than isoform a.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.