GALK2 (NM_001289030) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	GK2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336101 representing NM_001289030. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGTTTAACTCCAAGTTTGGATCTATTCCCAAGTTTTATGTTCGAGCACCAGGAAGAGTCAACATAATA GGAGAGCATATAGATTATTGTGGATATTCTGTTCTTCCTATGGCTGTAGAACAAGATGTGCTAATAGCT GTAGAACCTGTGAAAACGTACGCTCTCCAACTGGCCAATACAAATCCCTTGTATCCGGACTTCAGTACT AGTGCTAATAACATCCAGATTGATAAAACCAAGCCTTTGTGGCACAACTATTTCTTATGTGGACTTAAA GGAATTCAGGAACACTTTGGTCTTAGTAACCTGACTGGAATGAACTGCCTGGTAGATGGAAATATCCCA CCAAGTTCTGGCCTCTCCAGCTCCAGTGCTTTGGTCTGTTGTGCTGGCTTGGTGACGCTCACAGTGCTG GGAAGGAATCTATCCAAGGTGGAACTTGCAGAAATCTGTGCCAAGAGTGAGCGTTACATTGGCACTGAA GGAGGAGGCATGGACCAGTCTATATCATTTCTTGCAGAAGAAGGAACTGCCAAGTTGATAGAATTTAGT CCTCTGAGGGCAACCGATGTAAAACTCCCAAGTGGAGCAGTGTTTGTGATTGCCAACAGTTGTGTGGAG ATGAATAAGGCAGCAACTTCCCATTTCAATATCAGGGTGATGGAGTGTCGGCTGGCTGCGAAGCTCCTG GCTAAATACAAAAGCTTGCAATGGGACAAAGTACTGAGGCTGGAGGAGGTGCAGGCTAAACTAGGGATT AGTCTAGAAGAAATGCTGTTGGTCACAGAAGATGCCCTTCATCCTGAACCCTATAACCCTGAGGAGATC TGCAGGTGTCTGGGAATTAGCCTGGAGGAACTCCGAACCCAAATCCTGAGTCCAAACACTCAAGATGTG CTCATCTTCAAACTCTATCAGCGGGCAAAGCATGTGTACAGCGAGGCTGCGCGAGTGCTCCAGTTTAAG AAGATATGTGAAGAAGCACCTGAAAACATGGTCCAGCTGCTGGGAGAGTTGATGAACCAGAGCCACATG AGCTGCCGGGACATGTATGAGTGCAGCTGCCCCGAGCTGGATCAGCTGGTGGACATCTGTCGGAAGTTT GGGGCTCAAGGGTCACGACTTACTGGAGCAGGATGGGGAGGCTGCACAGTATCAATGGTACCTGCGGAC AAGCTGCCCAGCTTTCTAGCAAATGTGCACAAAGCTTATTACCAGAGGAGTGATGGAAGCTTAGCACCG GAGAAGCAAAGTTTGTTTGCTACCAAACCTGGAGGTGGGGCTTTGGTTTTGCTTGAGGCCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001289030
Insert Size	1305 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001289030.1
RefSeq Size	3460 bp
RefSeq ORF	1305 bp
Locus ID	2585
Protein Families	Druggable Genome
Protein Pathways	Amino sugar and nucleotide sugar metabolism, Galactose metabolism, Metabolic pathways
MW	47.6 kDa
Gene Summary	This gene encodes a highly efficient N-acetylgalactosamine (GalNAc) kinase, which has galactokinase activity when galactose is present at high concentrations. The encoded protein is a member of the GHMP kinase family. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2017] Transcript Variant: This variant (3) contains an alternate 5' exon and uses a downstream translation start codon, compared to variant 1. The encoded protein (isoform 3) has a shorter N-terminus, compared to isoform 1. Variants 3 and 4 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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