Synaptotagmin 1 (SYT1) (NM_001291901) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	BAGOS; P65; SVP65; SYT
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336010 representing NM_001291901. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGTGAGCGAGAGTCACCATGAGGCCCTGGCAGCCCCGCCTGTCACCACTGTCGCGACTGTTCTGCCA AGCAACGCCACAGAGCCAGCCAGTCCTGGAGAAGGAAAGGAAGATGCATTTTCTAAGCTGAAGGAGAAG TTTATGAATGAGTTGCATAAAATTCCATTGCCACCGTGGGCCTTAATTGCAATAGCCATAGTCGCAGTC CTTTTAGTCCTGACCTGCTGCTTTTGTATCTGTAAGAAATGTTTGTTCAAAAAGAAAAACAAGAAGAAG GGAAAGGAAAAAGGAGGGAAGAATGCCATTAACATGAAAGATGTAAAAGACTTAGGGAAGACGATGAAA GATCAGGATGATGATGCTGAAACTGGATTGACAGATGGAGAAGAAAAAGAAGAACCCAAAGAAGAGGAG AAACTGGGAAAACTTCAGTATTCACTGGATTATGATTTCCAAAATAACCAGCTGCTGGTAGGGATCATT CAGGCTGCCGAACTGCCCGCCTTGGACATGGGGGGCACATCTGATCCTTACGTGAAAGTGTTTCTGCTA CCTGATAAGAAGAAGAAATTTGAGACAAAAGTCCACCGAAAAACCCTTAATCCTGTCTTCAATGAGCAA TTTACTTTCAAGGTACCATACTCGGAATTGGGTGGCAAAACCCTAGTGATGGCTGTATATGATTTTGAT CGTTTCTCTAAGCATGACATCATTGGAGAATTTAAAGTCCCTATGAACACAGTGGATTTTGGCCATGTA ACTGAGGAATGGCGTGACCTGCAAAGTGCTGAGAAGGAAGAGCAAGAGAAATTGGGTGATATCTGCTTC TCCCTTCGCTACGTACCTACTGCTGGTAAGCTGACTGTTGTCATTCTGGAGGCAAAGAACCTGAAGAAG ATGGATGTGGGTGGCTTATCCGATCCTTATGTGAAGATTCATCTGATGCAGAATGGTAAGAGGCTGAAG AAGAAAAAGACAACAATTAAAAAGAACACACTTAACCCCTACTACAATGAGTCATTCAGCTTTGAAGTA CCTTTTGAACAAATCCAGAAAGTGCAGGTGGTGGTAACTGTTTTGGACTATGACAAGATTGGCAAGAAC GATGCCATCGGCAAAGTCTTTGTGGGCTACAACAGCACCGGCGCGGAGCTGCGACACTGGTCAGACATG CTGGCCAACCCCAGGCGACCTATTGCCCAGTGGCACACCCTGCAGGTAGAGGAGGAAGTTGATGCCATG CTGGCCGTCAAGAAGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001291901
Insert Size	1260 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001291901.1
RefSeq Size	4752 bp
RefSeq ORF	1260 bp
Locus ID	6857
Protein Families	Secreted Protein, Transmembrane
MW	47.3 kDa
Gene Summary	The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Calcium binding to synaptotagmin-1 participates in triggering neurotransmitter release at the synapse (Fernandez-Chacon et al., 2001 [PubMed 11242035]).[supplied by OMIM, Jul 2010] Transcript Variant: This variant (4) differs in the 5' UTR and uses an alternate in-frame splice site in the central coding region compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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