ATG4D (NM_001281504) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	APG4-D; APG4D; AUTL4
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC335944 representing NM_001281504. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGCAAGTGCTTCATCTCGCTGGGCGCTGCCCCTACGTCTCCCCAGGTTGGGTGGTTAAAAGCCGGACC AGCTTTAGCAAGATCTCCAGCATCCACCTCTGTGGCCGCCGCTACCGTTTCGAGGGCGAGGGTGACATA CAGCGTTTCCAGCGGGACTTTGTGTCCCGCCTGTGGCTCACATACCGCCGGGACTTCCCGCCCCTTCCT GGGGGCTGCCTGACCTCGGACTGTGGCTGGGGGTGCATGTTACGCAGCGGCCAGATGATGCTGGCACAG GGCCTTCTGCTGCATTTCCTGCCCAGAGACTGGACATGGGCCGAGGGCATGGGCCTGGGCCCCCCTGAG CTGTCAGGGTCAGCCTCTCCCAGCCGGTACCATGGGCCTGCCCGCTGGATGCCCCCACGCTGGGCCCAG GGTGCCCCTGAGCTGGAGCAGGAACGCCGGCACCGGCAGATTGTGTCCTGGTTCGCCGACCACCCCCGG GCCCCCTTTGGCCTACACCGGCTGGTGGAGCTTGGGCAGAGCTCAGGCAAGAAGGCAGGTGACTGGTAT GGGCCATCGCTAGTGGCACACATCCTCAGGAAAGCCGTGGAGAGCTGCTCCGACGTCACCCGCCTGGTG GTGTACGTTTCTCAGGACTGCACAGTGTACAAGGCGGATGTGGCACGCCTGGTGGCCAGGCCAGACCCC ACAGCCGAGTGGAAGTCTGTGGTCATCCTGGTGCCCGTGCGACTGGGTGGCGAGACTCTCAACCCCGTG TATGTGCCCTGCGTGAAGGAACTCCTGCGTTGCGAGCTGTGCCTGGGCATCATGGGTGGGAAACCGCGA CACTCACTGTACTTCATTGGCTACCAAGATGACTTCCTGCTGTACCTGGACCCTCACTACTGCCAGCCC ACTGTGGATGTCAGCCAGGCCGACTTCCCCCTGGAGTCCTTCCACTGCACCTCGCCCCGCAAGATGGCC TTTGCCAAGATGGACCCAAGCTGTACCGTGGGCTTCTATGCTGGAGACAGGAAGGAGTTTGAGACACTC TGCTCAGAGCTGACCAGGGTCCTCAGCTCCTCCTCAGCCACAGAGCGGTACCCCATGTTCACCCTGGCC GAGGGCCATGCTCAGGACCACAGCCTGGACGACCTCTGCTCCCAGCTCGCCCAGCCCACACTCCGGCTC CCTCGCACAGGGCGGCTCCTCAGGGCCAAACGCCCCAGCTCTGAGGACTTTGTGTTTTTATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001281504
Insert Size	1236 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001281504.1
RefSeq Size	2070 bp
RefSeq ORF	1236 bp
Locus ID	84971
UniProt ID	Q86TL0
Protein Pathways	Regulation of autophagy
MW	46.4 kDa
Gene Summary	Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene belongs to the autophagy-related protein 4 (Atg4) family of C54 endopeptidases. Members of this family encode proteins that play a role in the biogenesis of autophagosomes, which sequester the cytosol and organelles for degradation by lysosomes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013] Transcript Variant: This variant (2) uses an alternate splice site in the 5' coding region and initiates translation at an alternate start codon, compared to variant 1. It encodes isoform 2, which has a shorter and distinct N-terminus, compared to isoform 1.

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