HSPBP1 (NM_001297600) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	FES1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC335908 representing NM_001297600. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGGGGGTCGCCGAGCTCCCCTGAAACGCCGCCTTCTCAGCAGACCCTCAAGAATCGACCCATCAGGA CGCCAGAGCTGCTTCAGCGGTGACCACCTTCTCCCTCTAACACATTCTTCCCTTCTTCACAAACGGCCC ATGTCAGACGAAGGCTCAAGGGGGAGCCGCCTGCCCCTGGCGCTGCCCCCGGCCTCCCAGGGTTGCTCT TCAGGGGGCGGCGGCGGCGGCTCCTCGGCTGGGGGCTCGGGCAATTCCCGGCCCCCACGCAACCTCCAA GGCTTGCTGCAGATGGCCATCACCGCGGGCTCTGAAGAGCCAGACCCTCCTCCAGAACCGATGAGTGAG GAGAGGCGTCAGTGGCTGCAGGAGGCCATGTCGGCTGCCTTCCGAGGCCAGCGGGAGGAGGTGGAGCAG ATGAAGAGCTGCCTCCGAGTGCTGTCACAGCCCATGCCCCCCACTGCTGGGGAGGCCGAGCAGGCGGCC GACCAGCAAGAGCGAGAGGGGGCCCTGGAGCTGCTGGCCGACCTGTGTGAGAACATGGACAATGCCGCA GACTTCTGCCAGCTGTCTGGCATGCACCTGCTGGTGGGCCGGTACCTGGAGGCGGGGGCTGCGGGACTG CGGTGGCGGGCGGCACAGCTCATCGGCACGTGCAGTCAGAACGTGGCAGCCATCCAGGAGCAGGTGCTG GGCCTGGGTGCCCTGCGTAAGCTGCTGCGGCTGCTGGACCGCGACGCCTGCGACACGGTGCGCGTCAAG GCCCTCTTCGCCATCTCCTGTCTGGTCCGAGAGCAGGAGGCTGGGCTGCTGCAGTTCCTCCGCCTGGAC GGCTTCTCTGTGTTGATGAGGGCCATGCAGCAGCAGGTGCAGAAGCTCAAGGTCAAATCAGCATTCCTG CTGCAGAACCTGCTGGTGGGCCACCCTGAACACAAAGGGACCCTGTGCTCCATGGGGATGGTCCAGCAG CTGGTGGCCCTGGTGCGGACAGAGCACAGCCCCTTCCACGAGCACGTGCTTGGAGCCCTGTGCAGCCTG GTGACAGACTTTCCGCAGGGTGTGCGCGAGTGTCGGGAGCCGGAACTGGGCCTGGAGGAGCTCCTCCGC CACCGCTGTCAGCTGCTGCAGCAGCATGAGGAGTACCAGGAGGAGCTGGAGTTCTGTGAAAAGCTGCTA CAGACCTGTTTCTCCAGCCCAGCGGACGACAGCATGGATCGGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001297600
Insert Size	1218 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001297600.1
RefSeq Size	1715 bp
RefSeq ORF	1218 bp
Locus ID	23640
UniProt ID	Q9NZL4
MW	44.5 kDa
Gene Summary	Inhibits HSPA1A chaperone activity by changing the conformation of the ATP-binding domain of HSPA1A and interfering with ATP binding. Interferes with ubiquitination mediated by STUB1 and inhibits chaperone-assisted degradation of immature CFTR.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) encodes the longer isoform (1).

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