LASS3 (CERS3) (NM_001290341) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ARCI9; LASS3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC335839 representing NM_001290341. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGCTTCACACCACTGCCAGAAGAGCTGCCAGAATGTTTTGGACGTTTAAAGAATGGTTCTGGTTGGAA AGATTCTGGCTTCCTCCAACAATAAAGTGGTCAGATCTTGAGGATCACGATGGACTCGTCTTTGTAAAA CCTTCTCATTTATACGTGACAATTCCATATGCTTTTCTCTTGCTGATTATCAGACGTGTATTTGAAAAA TTTGTTGCTTCACCTCTAGCAAAATCATTTGGCATTAAAGAGACAGTTCGAAAGGTTACACCAAATACT GTCTTAGAGAATTTTTTCAAACATTCCACAAGGCAACCATTGCAAACTGATATTTATGGACTGGCAAAG AAGTGTAACTTGACGGAGCGCCAGGTGGAAAGATGGTTTAGGAGTCGGCGGAATCAAGAGAGGCCTTCC AGGCTGAAGAAATTCCAGGAAGCTTGCTGGAGATTTGCATTTTACTTAATGATCACTGTTGCTGGAATT GCGTTTCTTTATGATAAACCTTGGCTATATGACTTATGGGAGGTTTGGAATGGCTATCCCAAACAGCCC CTGCTGCCATCCCAGTACTGGTACTACATTTTAGAAATGAGTTTTTATTGGTCTCTGTTATTTAGACTT GGCTTTGATGTCAAGAGAAAGGATTTTCTAGCTCATATCATCCACCACCTGGCTGCTATTAGTCTGATG AGCTTCTCTTGGTGTGCTAATTATATTCGCAGTGGGACCCTCGTGATGATTGTACACGATGTGGCTGAC ATTTGGCTGGAGTCTGCTAAGATGTTTTCTTATGCTGGATGGACGCAGACCTGTAACACCCTGTTTTTC ATCTTCTCCACCATATTTTTCATCAGCCGCCTCATTGTTTTTCCTTTCTGGATTTTATATTGCACGCTG ATCTTGCCTATGTATCACCTCGAGCCTTTCTTTTCATACATCTTCCTCAACCTACAGCTCATGATCTTG CAGGTCCTTCACCTTTACTGGGGTTATTACATCTTGAAGATGCTCAACAGATGTATATTCATGAAGAGC ATCCAGGATGTGAGGAGTGATGACGAGGATTATGAAGAGGAAGAGGAAGAGGAAGAAGAAGAGGCTACC AAAGGCAAAGAGATGGATTGTTTAAAGAACGGCCTCAGGGCTGAGAGGCACCTCATTCCCAATGGCCAG CATGGCCATTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001290341
Insert Size	1185 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001290341.2
RefSeq Size	4176 bp
RefSeq ORF	1185 bp
Locus ID	204219
UniProt ID	Q8IU89
Protein Families	Transcription Factors, Transmembrane
MW	47.6 kDa
Gene Summary	This gene is a member of the ceramide synthase family of genes. The ceramide synthase enzymes regulate sphingolipid synthesis by catalyzing the formation of ceramides from sphingoid base and acyl-coA substrates. This family member is involved in the synthesis of ceramides with ultra-long-chain acyl moieties (ULC-Cers), important to the epidermis in its role in creating a protective barrier from the environment. The protein encoded by this gene has also been implicated in modification of the lipid structures required for spermatogenesis. Mutations in this gene have been associated with male fertility defects, and epidermal defects, including ichthyosis. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2015] Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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