LASS3 (CERS3) (NM_001290341) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	ARCI9; LASS3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC237945 representing NM_001290341 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTTCACACCACTGCCAGAAGAGCTGCCAGAATGTTTTGGACGTTTAAAGAATGGTTCTGGTTGGAAA GATTCTGGCTTCCTCCAACAATAAAGTGGTCAGATCTTGAGGATCACGATGGACTCGTCTTTGTAAAACC TTCTCATTTATACGTGACAATTCCATATGCTTTTCTCTTGCTGATTATCAGACGTGTATTTGAAAAATTT GTTGCTTCACCTCTAGCAAAATCATTTGGCATTAAAGAGACAGTTCGAAAGGTTACACCAAATACTGTCT TAGAGAATTTTTTCAAACATTCCACAAGGCAACCATTGCAAACTGATATTTATGGACTGGCAAAGAAGTG TAACTTGACGGAGCGCCAGGTGGAAAGATGGTTTAGGAGTCGGCGGAATCAAGAGAGGCCTTCCAGGCTG AAGAAATTCCAGGAAGCTTGCTGGAGATTTGCATTTTACTTAATGATCACTGTTGCTGGAATTGCGTTTC TTTATGATAAACCTTGGCTATATGACTTATGGGAGGTTTGGAATGGCTATCCCAAACAGCCCCTGCTGCC ATCCCAGTACTGGTACTACATTTTAGAAATGAGTTTTTATTGGTCTCTGTTATTTAGACTTGGCTTTGAT GTCAAGAGAAAGGATTTTCTAGCTCATATCATCCACCACCTGGCTGCTATTAGTCTGATGAGCTTCTCTT GGTGTGCTAATTATATTCGCAGTGGGACCCTCGTGATGATTGTACACGATGTGGCTGACATTTGGCTGGA GTCTGCTAAGATGTTTTCTTATGCTGGATGGACGCAGACCTGTAACACCCTGTTTTTCATCTTCTCCACC ATATTTTTCATCAGCCGCCTCATTGTTTTTCCTTTCTGGATTTTATATTGCACGCTGATCTTGCCTATGT ATCACCTCGAGCCTTTCTTTTCATACATCTTCCTCAACCTACAGCTCATGATCTTGCAGGTCCTTCACCT TTACTGGGGTTATTACATCTTGAAGATGCTCAACAGATGTATATTCATGAAGAGCATCCAGGATGTGAGG AGTGATGACGAGGATTATGAAGAGGAAGAGGAAGAGGAAGAAGAAGAGGCTACCAAAGGCAAAGAGATGG ATTGTTTAAAGAACGGCCTCAGGGCTGAGAGGCACCTCATTCCCAATGGCCAGCATGGCCAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC237945 representing NM_001290341 Red=Cloning site Green=Tags(s) MLHTTARRAARMFWTFKEWFWLERFWLPPTIKWSDLEDHDGLVFVKPSHLYVTIPYAFLLLIIRRVFEKF VASPLAKSFGIKETVRKVTPNTVLENFFKHSTRQPLQTDIYGLAKKCNLTERQVERWFRSRRNQERPSRL KKFQEACWRFAFYLMITVAGIAFLYDKPWLYDLWEVWNGYPKQPLLPSQYWYYILEMSFYWSLLFRLGFD VKRKDFLAHIIHHLAAISLMSFSWCANYIRSGTLVMIVHDVADIWLESAKMFSYAGWTQTCNTLFFIFST IFFISRLIVFPFWILYCTLILPMYHLEPFFSYIFLNLQLMILQVLHLYWGYYILKMLNRCIFMKSIQDVR SDDEDYEEEEEEEEEEATKGKEMDCLKNGLRAERHLIPNGQHGH TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001290341
ORF Size	1182 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001290341.2, NP_001277270.1
RefSeq Size	4089 bp
RefSeq ORF	1185 bp
Locus ID	204219
UniProt ID	Q8IU89
Protein Families	Transcription Factors, Transmembrane
MW	48 kDa
Gene Summary	This gene is a member of the ceramide synthase family of genes. The ceramide synthase enzymes regulate sphingolipid synthesis by catalyzing the formation of ceramides from sphingoid base and acyl-coA substrates. This family member is involved in the synthesis of ceramides with ultra-long-chain acyl moieties (ULC-Cers), important to the epidermis in its role in creating a protective barrier from the environment. The protein encoded by this gene has also been implicated in modification of the lipid structures required for spermatogenesis. Mutations in this gene have been associated with male fertility defects, and epidermal defects, including ichthyosis. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2015]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.