MFSD2A (NM_001287808) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	MCPH15; MFSD2; NEDMISBA; NLS1; SLC59A1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC335712 representing NM_001287808. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGTCACGTGTTTCCATGTTCCCTACTCGGCTCTCACCATGTTCATCAGCACCGAGCAGACTGAGCGG GATTCTGCCACCGCCTATCGGATGACTGTGGAAGTGCTGGGCACAGTGCTGGGCACGGCGATCCAGGGA CAAATCGTGGGCCAAGCAGACACGCCTTGTTTCCAGGACCTCAATAGCTCTACAGTAGCTTCACAAAGT GCCAACCATACACATGGCACCACCTCACACAGGGAAACGCAAAAGGCATACCTGCTGGCAGCGGGGGTC ATTGTCTGTATCTATATAATCTGTGCTGTCATCCTGATCCTGGGCGTGCGGGAGCAGAGAGAACCCTAT GAAGCCCAGCAGTCTGAGCCAATCGCCTACTTCCGGGGCCTACGGCTGGTCATGAGCCACGGCCCATAC ATCAAACTTATTACTGGCTTCCTCTTCACCTCCTTGGCTTTCATGCTGGTGGAGGGGAACTTTGTCTTG TTTTGCACCTACACCTTGGGCTTCCGCAATGAATTCCAGAATCTACTCCTGGCCATCATGCTCTCGGCC ACTTTAACCATTCCCATCTGGCAGTGGTTCTTGACCCGGTTTGGCAAGAAGACAGCTGTATATGTTGGG ATCTCATCAGCAGTGCCATTTCTCATCTTGGTGGCCCTCATGGAGAGTAACCTCATCATTACATATGCG GTAGCTGTGGCAGCTGGCATCAGTGTGGCAGCTGCCTTCTTACTACCCTGGTCCATGCTGCCTGATGTC ATTGACGACTTCCATCTGAAGCAGCCCCACTTCCATGGAACCGAGCCCATCTTCTTCTCCTTCTATGTC TTCTTCACCAAGTTTGCCTCTGGAGTGTCACTGGGCATTTCTACCCTCAGTCTGGACTTTGCAGGGTAC CAGACCCGTGGCTGCTCGCAGCCGGAACGTGTCAAGTTTACACTGAACATGCTCGTGACCATGGCTCCC ATAGTTCTCATCCTGCTGGGCCTGCTGCTCTTCAAAATGTACCCCATTGATGAGGAGAGGCGGCGGCAG AATAAGAAGGCCCTGCAGGCACTGAGGGACGAGGCCAGCAGCTCTGGCTGCTCAGAAACAGACTCCACA GAGCTGGCTAGCATCCTCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001287808
Insert Size	1125 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001287808.1
RefSeq Size	1932 bp
RefSeq ORF	1125 bp
Locus ID	84879
UniProt ID	Q8NA29
Protein Families	Transmembrane
MW	41.4 kDa
Gene Summary	The protein encoded by this gene is a transmembrane protein and sodium-dependent lysophosphatidylcholine transporter. The encoded protein is involved in the establishment of the blood-brain barrier and is required for brain growth and function. Defects in this gene are a cause of a progressive microcephaly syndrome. [provided by RefSeq, Mar 2017] Transcript Variant: This variant (3) differs in its 5' UTR, and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (3) has a shorter N-terminus, compared to isoform 1.

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