GMPR2 (NM_001283021) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	GMPR 2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC335708 representing NM_001283021. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGGATGTGTTTTTCTTATATACAAGTTGTTCACTTTGAAATGGAAGATGCTGCTCCTGTCAGTACTA TTACCTGCCTCTATACTTGTTGCTGAGAAGTTCTCTCTCTTCACTGCTGTCCATAAGCACTATAGCCTC GTTCAGTGGCAAGAGTTTGCTGGCCAGAATCCTGACTGTCTTGAGCATCTGGCTGCCAGCTCAGGCACA GGCTCTTCTGACTTTGAGCAGCTGGAACAGATCCTGGAAGCTATTCCCCAGGTGAAGTATATATGCCTG GATGTGGCAAATGGCTACTCTGAACACTTTGTTGAATTTGTAAAAGATGTACGGAAGCGCTTCCCCCAG CACACCATCATGGCAGGGAATGTGGTAACAGGAGAGATGGTAGAAGAGCTCATCCTTTCTGGGGCTGAC ATCATCAAAGTGGGAATTGGGCCAGGCTCTGTGTGTACTACTCGGAAGAAAACTGGAGTGGGGTATCCA CAGCTCAGCGCAGTGATGGAGTGTGCAGATGCTGCTCATGGCCTCAAAGGCCACATCATTTCAGATGGA GGTTGCAGCTGTCCTGGGGATGTGGCCAAGGCTTTTGGGGCAGGAGCTGACTTCGTGATGCTGGGTGGC ATGCTGGCTGGGCACAGTGAGTCAGGTGGTGAGCTCATCGAGAGGGATGGCAAGAAGTACAAGCTCTTC TATGGAATGAGTTCTGAAATGGCCATGAAGAAGTATGCTGGGGGCGTGGCTGAGTACAGGTATGTGTGG AGGCCCAGGAGCTTAGTAATAGTATGGAGGCAGAACTCATGGCTGCTGAGAGGGGGATGGTACAGTTCT CAGAGAAGCATGGTGAACCGGGGCTCAATGCTAGGGTCTGTGGAAAAGTCCCTGGGCTTAAGGAATCCA GAAGGAGAAGATAATAAAGTTTTTCCTACTTTAAGAGCCTCAGAGGGAAAGACAGTGGAAGTTCCTTTT AAAGGAGATGTGGAACATACCATCCGAGACATCCTAGGAGGGATCCGCTCTACGTGTACCTATGTGGGA GCAGCTAAGCTCAAAGAGTTGAGCAGGAGAACTACCTTCATCCGAGTCACCCAGCAGGTGAATCCAATC TTCAGTGAGGCGTGCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001283021
Insert Size	1122 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001283021.1
RefSeq Size	2248 bp
RefSeq ORF	1122 bp
Locus ID	51292
UniProt ID	Q9P2T1
Protein Families	Druggable Genome
Protein Pathways	Purine metabolism
MW	40.9 kDa
Gene Summary	This gene encodes an enzyme that catalyzes the irreversible and NADPH-dependent reductive deamination of guanosine monophosphate (GMP) to inosine monophosphate (IMP). The protein also functions in the re-utilization of free intracellular bases and purine nucleosides. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2017] Transcript Variant: This variant (5) differs in the 5' UTR and contains multiple differences in the coding region, compared to variant 1, including initiation of translation at an alternate start codon. The encoded isoform (3) is longer and has a distinct N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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