FBXO31 (NM_001282683) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	FBX14; Fbx31; FBXO14; MRT45; pp2386
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC335654 representing NM_001282683. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGTACCTGCCTCCCCATGACCCCCACGTCGATGACCCTATGAGATTCAAGCCTCTGTTCAGGATCCAC CTGATGGAGAGGAAGGCTGCCACAGTGGAGTGCATGTACGGCCACAAAGGGCCCCACCACGGCCACATC CAGATTGTGAAGAAGGATGAGTTCTCCACCAAGTGCAACCAGACGGACCACCACAGGATGTCCGGCGGG AGGCAGGAGGAGTTTCGGACGTGGCTGAGGGAGGAATGGGGGCGCACGCTGGAGGACATCTTCCACGAG CACATGCAGGAGCTCATCCTGATGAAGTTCATCTACACCAGTCAGTACGACAACTGCCTGACCTACCGC CGCATCTACCTGCCGCCCAGCCGCCCCGACGACCTCATCAAGCCTGGCCTCTTCAAAGGTACCTATGGC AGCCACGGCCTGGAGATTGTGATGCTCAGCTTCCACGGCCGGCGTGCCAGGGGCACCAAGATCACGGGC GACCCCAACATCCCCGCTGGGCAGCAGACAGTGGAGATCGACCTGAGGCATCGGATCCAGCTGCCCGAC CTCGAGAACCAGCGCAACTTCAATGAGCTCTCCCGCATCGTCCTGGAGGTGCGCGAGAGGGTGCGCCAG GAGCAGCAGGAAGGCGGGCACGAGGCGGGCGAGGGTCGTGGCCGGCAGGGCCCCCGGGAGTCCCAGCCA AGCCCTGCCCAGCCCAGGGCAGAGGCGCCCAGCAAGGGCCCAGATGGGACACCTGGTGAGGATGGTGGC GAGCCTGGGGATGCCGTAGCTGCGGCCGAGCAGCCTGCCCAGTGTGGGCAGGGGCAGCCGTTCGTGCTG CCCGTGGGCGTGAGCTCCAGGAATGAGGACTACCCCCGAACCTGCAGGATGTGTTTTTATGGCACAGGC CTCATCGCGGGCCACGGCTTCACCAGCCCTGAACGCACCCCCGGGGTCTTCATCCTCTTCGATGAGGAC CGCTTCGGGTTCGTCTGGCTGGAGCTGAAATCCTTCAGCCTGTACAGCCGGGTCCAGGCCACCTTCCGG AACGCAGATGCGCCGTCCCCACAGGCCTTCGATGAGATGCTCAAGAACATTCAGTCCCTCACCTCCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001282683
Insert Size	1104 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001282683.1
RefSeq Size	5738 bp
RefSeq ORF	1104 bp
Locus ID	79791
UniProt ID	Q5XUX0
Protein Families	Druggable Genome
MW	41.7 kDa
Gene Summary	This gene is a member of the F-box family. Members are classified into three classes according to the substrate interaction domain, FBW for WD40 repeats, FBL for leucing-rich repeats, and FBXO for other domains. This protein, classified into the last category because of the lack of a recognizable substrate binding domain, has been proposed to be a component of the SCF ubiquitination complex. It is thought to bind and recruit substrate for ubiquitination and degradation. This protein may have a role in regulating the cell cycle as well as dendrite growth and neuronal migration. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013] Transcript Variant: This variant (2) represents use of an alternate promoter and thus differs in the 5' UTR and 5' coding region compared to variant 1. These differences cause translation initiation at a downstream AUG and result in an isoform (2) with a shorter N-terminus, compared to isoform 1.

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