FBXO31 (NM_001282683) Human Tagged ORF Clone
CAT#: RC237760
- TrueORF®
FBXO31 (myc-DDK-tagged) - Human F-box protein 31 (FBXO31), transcript variant 2
ORF Plasmid: tGFP
"NM_001282683" in other vectors (2)
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CNY 3,990.00
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | FBX14; Fbx31; FBXO14; MRT45; pp2386 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC237760 representing NM_001282683
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTACCTGCCTCCCCATGACCCCCACGTCGATGACCCTATGAGATTCAAGCCTCTGTTCAGGATCCACC TGATGGAGAGGAAGGCTGCCACAGTGGAGTGCATGTACGGCCACAAAGGGCCCCACCACGGCCACATCCA GATTGTGAAGAAGGATGAGTTCTCCACCAAGTGCAACCAGACGGACCACCACAGGATGTCCGGCGGGAGG CAGGAGGAGTTTCGGACGTGGCTGAGGGAGGAATGGGGGCGCACGCTGGAGGACATCTTCCACGAGCACA TGCAGGAGCTCATCCTGATGAAGTTCATCTACACCAGTCAGTACGACAACTGCCTGACCTACCGCCGCAT CTACCTGCCGCCCAGCCGCCCCGACGACCTCATCAAGCCTGGCCTCTTCAAAGGTACCTATGGCAGCCAC GGCCTGGAGATTGTGATGCTCAGCTTCCACGGCCGGCGTGCCAGGGGCACCAAGATCACGGGCGACCCCA ACATCCCCGCTGGGCAGCAGACAGTGGAGATCGACCTGAGGCATCGGATCCAGCTGCCCGACCTCGAGAA CCAGCGCAACTTCAATGAGCTCTCCCGCATCGTCCTGGAGGTGCGCGAGAGGGTGCGCCAGGAGCAGCAG GAAGGCGGGCACGAGGCGGGCGAGGGTCGTGGCCGGCAGGGCCCCCGGGAGTCCCAGCCAAGCCCTGCCC AGCCCAGGGCAGAGGCGCCCAGCAAGGGCCCAGATGGGACACCTGGTGAGGATGGTGGCGAGCCTGGGGA TGCCGTAGCTGCGGCCGAGCAGCCTGCCCAGTGTGGGCAGGGGCAGCCGTTCGTGCTGCCCGTGGGCGTG AGCTCCAGGAATGAGGACTACCCCCGAACCTGCAGGATGTGTTTTTATGGCACAGGCCTCATCGCGGGCC ACGGCTTCACCAGCCCTGAACGCACCCCCGGGGTCTTCATCCTCTTCGATGAGGACCGCTTCGGGTTCGT CTGGCTGGAGCTGAAATCCTTCAGCCTGTACAGCCGGGTCCAGGCCACCTTCCGGAACGCAGATGCGCCG TCCCCACAGGCCTTCGATGAGATGCTCAAGAACATTCAGTCCCTCACCTCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC237760 representing NM_001282683
Red=Cloning site Green=Tags(s) MYLPPHDPHVDDPMRFKPLFRIHLMERKAATVECMYGHKGPHHGHIQIVKKDEFSTKCNQTDHHRMSGGR QEEFRTWLREEWGRTLEDIFHEHMQELILMKFIYTSQYDNCLTYRRIYLPPSRPDDLIKPGLFKGTYGSH GLEIVMLSFHGRRARGTKITGDPNIPAGQQTVEIDLRHRIQLPDLENQRNFNELSRIVLEVRERVRQEQQ EGGHEAGEGRGRQGPRESQPSPAQPRAEAPSKGPDGTPGEDGGEPGDAVAAAEQPAQCGQGQPFVLPVGV SSRNEDYPRTCRMCFYGTGLIAGHGFTSPERTPGVFILFDEDRFGFVWLELKSFSLYSRVQATFRNADAP SPQAFDEMLKNIQSLTS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_001282683 |
ORF Size | 1101 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001282683.2 |
RefSeq Size | 5738 bp |
RefSeq ORF | 1104 bp |
Locus ID | 79791 |
UniProt ID | Q5XUX0 |
Protein Families | Druggable Genome |
MW | 42.2 kDa |
Gene Summary | This gene is a member of the F-box family. Members are classified into three classes according to the substrate interaction domain, FBW for WD40 repeats, FBL for leucing-rich repeats, and FBXO for other domains. This protein, classified into the last category because of the lack of a recognizable substrate binding domain, has been proposed to be a component of the SCF ubiquitination complex. It is thought to bind and recruit substrate for ubiquitination and degradation. This protein may have a role in regulating the cell cycle as well as dendrite growth and neuronal migration. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013] |
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