ZNF124 (NM_001297568) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	HZF-16; HZF16; ZK7
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC335549 representing NM_001297568. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGTCGGGACACCCCGGAAGCTGGGAAATGAACTCGGTTGCCTTTGAGGATGTGGCTGTGAACTTCACC CAGGAGGAGTGGGCTTTGTTGGATCCTTCCCAGAAGAATCTCTATAGAGACGTGATGCAGGAAACCTTC AGGAATCTGGCTTCCATAGGAAACAAAGGGGAAGACCAGAGCATTGAAGATCAGTACAAAAATTCTTCA AGAAATCTAAGGCACATCATATCTCATTCTGGAAACAACCCATATGGGTGTGAGGAATGCGGAAAGAAG CCATGTACATGTAAACAATGTCAGAAAACTTCCCTTTCTGTCACAAGGGTTCACAGAGACACAGTAATG CACACTGGAAATGGACATTATGGTTGTACAATATGTGAGAAAGTTTTTAATATTCCCAGTTCATTTCAG ATACATCAGAGAAATCACACTGGAGAGAAACCCTATGAATGTATGGAATGTGGGAAAGCCTTAGGTTTT TCCCGTTCTCTTAATAGACATAAAAGGATTCACACTGGAGAAAAACGCTATGAATGTAAGCAATGTGGG AAAGCCTTCAGTCGTTCCAGTCACCTTCGTGACCATGAAAGAACTCATACTGGAGAGAAACCCTATGAA TGTAAGCACTGTGGGAAAGCCTTCCGTTACTCCAATTGCCTTCATTACCATGAAAGAACTCACACTGGA GAGAAACCTTATGTGTGCATGGAATGTGGCAAAGCTTTCAGTTGTCTCAGTTCCTTGCAAGGACATATA AAGGCTCATGCTGGTGAAGAACCCTATCCATGTAAGCAATGTGGGAAAGCCTTCAGATACGCCAGTTCC CTTCAGAAACACGAGAAAACTCATATTGCACAGAAACCCTATGTATGTAACAATTGTGGTAAAGGCTTC AGATGTTCCAGTTCCCTTCGTGACCATGAAAGGACTCATACTGGAGAGAAACCCTATGAATGTCAGAAA TGTGGCAAAGCCTTTAGTCGTGCTAGTACCCTTTGGAAGCATAAAAAAACTCATACTGGAGAAAAGCCC TATAAATGTAAAAAAATGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001297568
Insert Size	1056 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001297568.1
RefSeq Size	2791 bp
RefSeq ORF	1056 bp
Locus ID	7678
UniProt ID	Q15973
MW	40.2 kDa
Gene Summary	This gene encodes a protein with an amino-terminal KRAB-A box and multiple repeated Kruppel-type (C2H2) zinc finger motifs at its carboxy terminus. The encoded protein may function as a transcription factor. Expression of this gene is increased after vascular endothelial growth factor (VEGF) stimulation in human leukemia cell lines and results in inhibition of apoptotic cell death induced by irradiation or exposure to etoposide. Alternative splicing results in multiple transcript variants encoding distinct proteins. [provided by RefSeq, Jul 2014] Transcript Variant: This variant (4) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. The encoded isoform (4) is longer than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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