SGK196 (POMK) (NM_001277971) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	MDDGA12; MDDGC12; SGK196
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC335533 representing NM_001277971. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGAAAAGCAGCCCCAGAACAGCAGGAGAGGCCTCGCCCCCCGAGAGGTGCCGCCAGCTGTTGGGCTG CTGCTGATCATGGCCCTGATGAATACTCTGCTCTACCTCTGCCTCGACCACTTCTTCATCGCTCCTCGA CAATCCACTGTGGACCCCACACACTGTCCCTATGGTCACTTCAGGATAGGACAGATGAAAAACTGCTCA CCTTGGCTGTCCTGCGAGGAGCTGAGAACAGAAGTGAGACAGCTGAAGCGTGTTGGGGAAGGAGCTGTA AAGAGAGTCTTTCTGTCTGAGTGGAAGGAGCACAAAGTTGCACTCTCACAGCTCACCAGCCTGGAGATG AAAGATGATTTCCTCCATGGACTGCAGATGCTGAAATCTCTCCAAGGCACACATGTTGTCACGCTGCTT GGCTATTGTGAGGATGACAACACTATGCTTACTGAATATCACCCTCTAGGTTCCTTGAGTAACCTGGAA GAAACACTAAACCTTTCAAAGTACCAAAATGTGAACACGTGGCAGCACAGGCTGGAGCTGGCCATGGAC TATGTCAGCATCATTAATTACCTGCACCACAGCCCTGTGGGCACACGGGTCATGTGCGACTCCAACGAC CTGCCGAAGACACTGTCCCAGTATCTGCTAACAAGCAACTTCAGCATTTTGGCAAATGACTTGGACGCC TTACCCCTGGTGAACCACAGCTCCGGGATGCTGGTGAAGTGCGGCCACAGGGAGCTGCATGGGGATTTC GTGGCTCCAGAGCAACTGTGGCCCTATGGAGAGGACGTGCCTTTCCACGATGATCTCATGCCCTCATAT GATGAGAAGATTGACATTTGGAAGATCCCAGACATCTCCAGTTTCCTTCTGGGGCACATTGAAGGGAGT GATATGGTCCGATTCCATTTGTTTGATATTCACAAAGCATGCAAGAGCCAGACTCCCTCAGAAAGACCC ACTGCCCAGGACGTTCTGGAGACCTACCAGAAGGTCTTGGATACACTTAGAGATGCCATGATGTCTCAG GCAAGAGAGATGCTGTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001277971
Insert Size	1053 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001277971.1
RefSeq Size	1527 bp
RefSeq ORF	1053 bp
Locus ID	84197
UniProt ID	Q9H5K3
Protein Families	Druggable Genome, Protein Kinase, Transmembrane
MW	40 kDa
Gene Summary	This gene encodes a protein that may be involved in the presentation of the laminin-binding O-linked carbohydrate chain of alpha-dystroglycan (a-DG), which forms transmembrane linkages between the extracellular matrix and the exoskeleton. Some pathogens use this O-linked carbohydrate unit for host entry. Loss of function compound heterozygous mutations in this gene were found in a human patient affected by the Walker-Warburg syndrome (WWS) phenotype. Mice lacking this gene contain misplaced neurons (heterotopia) in some regions of the brain, possibly from defects in neuronal migration. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, May 2013] Transcript Variant: This variant (2) lacks an alternate exon in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same protein.

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