EXTL2 (NM_001261441) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	EXTR2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC335451 representing NM_001261441. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGAGAACCTGGAACAGTGGTGGCACAAAGTGTTGCCACATCTGCAAACTTCCTGGGAGAGTAATGGGG ATTCGAGTGCTTCGATTATCTTTGGTGGTCATCCTCGTATTATTACTGGTAGCTGGTGCTTTGACTGCC TTACTTCCCAGTGTTAAAGAAGACAAGATGCTCATGTTGCGTAGGGAAATAAAATCCCAGGGCAAGTCC ACCATGGACTCCTTTACTCTCATAATGCAGACGTACAACAGAACAGATCTCTTATTGAAACTTTTAAAT CATTATCAGGCTGTACCAAATCTGCACAAAGTGATTGTGGTATGGAACAATATTGGAGAGAAGGCACCA GATGAATTATGGAATTCTCTAGGGCCCCACCCTATCCCTGTGATCTTCAAACAACAGACAGCAAACAGG ATGAGAAATCGACTCCAGGTCTTTCCTGAACTGGAAACCAATGCAGTGTTGATGGTAGATGATGACACA CTCATCAGCACCCCAGACCTTGTTTTTGCTTTCTCAGTTTGGCAGCAATTTCCTGATCAAATTGTAGGA TTTGTTCCTAGAAAGCACGTCTCTACTTCATCAGGTATCTACAGTTATGGAAGTTTTGAAATGCAAGCA CCAGGGTCTGGAAATGGTGACCAGTACTCTATGGTGCTGATTGGAGCCTCATTCTTCAATAGCAAATAT CTTGAATTATTTCAGAGGCAACCTGCAGCTGTCCATGCTTTGATAGATGATACTCAAAACTGTGATGAT ATTGCCATGAATTTTATCATTGCCAAGCATATTGGCAAGACTTCAGGGATATTTGTGAAGCCTGTAAAC ATGGACAATTTGGAAAAAGAAACCAACAGTGGCTATTCTGGAATGTGGCATCGAGCTGAGCACGCTCTG CAGAGGTCTTATTGTATAAATAAGCTTGTTAATATCTATGATAGCATGCCCTTAAGATACTCCAACATT ATGATTTCCCAGTTTGGTTTTCCATATGCCAACTACAAAAGAAAAATATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001261441
Insert Size	1017 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001261441.1
RefSeq Size	3205 bp
RefSeq ORF	1017 bp
Locus ID	2135
Protein Families	Transmembrane
Protein Pathways	Heparan sulfate biosynthesis
MW	38.3 kDa
Gene Summary	Glycosyltransferase required for the biosynthesis of heparan-sulfate and responsible for the alternating addition of beta-1-4-linked glucuronic acid (GlcA) and alpha-1-4-linked N-acetylglucosamine (GlcNAc) units to nascent heparan sulfate chains.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (4) uses an alternate splice site in the 5' UTR and includes an alternate exon in the coding region, but maintains the reading frame, compared to variant 1. The encoded isoform (3) is longer than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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