NMNAT1 (NM_001297778) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	LCA9; NMNAT; PNAT1; SHILCA
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene sequence for NM_022787 edited AGAGTGCGACCGAGATGTTCCACTCGCTGGCGTCCGGGCCGCTGGTGATCTCCGGTAGCA CTCGGGCCGGCGGACAGTGAGGGCGCGACAACAAGGGAGGTGTCACAGTTTTCCATTTAG ATCAACAACTTCAAGTTCTTACCATGGAAAATTCCGAGAAGACTGAAGTGGTTCTCCTTG CTTGTGGTTCATTCAATCCCATCACCAACATGCACCTCAGGTTGTTTGAGCTGGCCAAGG ACTACATGAATGGAACAGGAAGGTACACAGTTGTCAAAGGCATCATCTCTCCTGTTGGTG ATGCCTACAAGAAGAAAGGACTCATTCCTGCCTATCACCGGGTCATCATGGCAGAACTTG CTACCAAGAATTCTAAATGGGTGGAAGTTGATACATGGGAAAGTCTTCAGAAGGAGTGGA AAGAGACTCTGAAGGTGCTAAGACACCATCAAGAGAAATTGGAGGCTAGTGACTGTGATC ACCAGCAGAACTCACCTACTCTAGAAAGGCCTGGAAGGAAGAGGAAGTGGACTGAAACAC AAGATTCTAGTCAAAAGAAATCCCTAGAGCCAAAAACAAAAGCTGTGCCAAAGGTCAAGC TGCTGTGTGGGGCAGATTTATTGGAGTCCTTTGCTGTTCCCAATTTGTGGAAGAGTGAAG ACATCACCCAAATCGTGGCCAACTATGGGCTCATATGTGTTACTCGGGCTGGAAATGATG CTCAGAAGTTTATCTATGAATCGGATGTGCTGTGGAAACACCGGAGCAACATTCACGTGG TGAATGAATGGATCGCTAATGACATCTCATCCACAAAAATCCGGAGAGCCCTCAGAAGGG GCCAGAGCATTCGCTACTTGGTACCAGATCTTGTCCAAGAATACATTGAAAAGCATAATT TGTACAGCTCTGAGAGTGAAGACAGGAATGCTGGGGTCATCCTGGCCCCTTTGCAGAGAA ACACTGCAGAAGCTAAGACATAGGAATTCTACAGCATGATATTTCAGACTTCCCATTTGG GGATCTGAAACAATCTGGGAGTTAATAACTGGGGAAAGAAGTTGTGATCTGTTGCCTAAA CTAAAGCTTAAAAGTTTAGTAAAAATCGTCTGGGCACAGTGGCTCACGCCTGTAGTCCCA GCTACTTGGGAGGCTGAGGCAGGAGAATCACTTGACCCCAGGTGGTGGAGGTTGCAGTGA GCCAAGATTGCACCATTGCACTCCAGCCTGGCGACAGAGCAAGACTCTGTCTCAAAAAAA AAAAAAAAATTTAGTAAAAATCAATGGTAAGCTAAAATAAGTTTTTGTTTGTTTATTTGT TTTTGAGATGGAGTCTCTACTAAAAATACAAAAAATTAGCCAGGCATGGTGCCGCATAAC TATAATCCCAGCTACTTGGGAGGCTGAGGCAGGAGAATCGCTTGAACCCGGGAGGCACAG GTTCCAGTGGGCCAAGGTTGTGCCACTGCACTCCAGCCTGGGCAAAAAAGCAAAACTCCA TCTCAAAGAGAAAAAAAAAAAAGACCGGGTGTGGTGGCTCACACCTGTAATCCCAGCACT TTGGGAGGCCTAAGTGGGTGGATCACGTGAGGTCAAGAGTTCAAGACCAGCCTGGCCAAT ATGGTGAAACCCCATCTCTACTAAGAATACAAAAAATTAGCTGAGCATGGTGGTGGGCTC CTGTAGTCCCAGCTACTTGGGAGGCTGAGGCAGGAGAATCGCTTGAACCTGGGAGGCAGA GGTTGCAGTAAGCCAAGATCGTGCCATTGCACTCCAGCCTGGGTGACAGAGCGAGACTCC ATCTCAAAAAAAAAAAAAAAAAAAA
Restriction Sites	SgfI-MluI
ACCN	NM_001297778
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001297778.1, NP_001284707.1
RefSeq Size	3796 bp
RefSeq ORF	840 bp
Locus ID	64802
UniProt ID	Q9HAN9
Protein Pathways	Metabolic pathways, Nicotinate and nicotinamide metabolism
Gene Summary	This gene encodes an enzyme which catalyzes a key step in the biosynthesis of nicotinamide adenine dinucleotide (NAD). The encoded enzyme is one of several nicotinamide nucleotide adenylyltransferases, and is specifically localized to the cell nucleus. Activity of this protein leads to the activation of a nuclear deacetylase that functions in the protection of damaged neurons. Mutations in this gene have been associated with Leber congenital amaurosis 9. Alternative splicing results in multiple transcript variants. Pseudogenes of this gene are located on chromosomes 1, 3, 4, 14, and 15. [provided by RefSeq, Jul 2014] Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Variants 1 and 2 encode the same isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

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