BXDC1 (RPF2) (NM_001289111) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	bA397G5.4; BXDC1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001289111, the custom clone sequence may differ by one or more nucleotides ATGTGGAAAAATATTACAAGACCTTTTGAGGATCAGACATCACTGGAATTCTTTTCAAAGAAGTCAGATT GTTCTTTATTCATGTTTGGCTCCCATAATAAGAAGCGGCCAAATAATCTAGTAATAGGTCGTATGTATGA CTACCATGTGCTGGATATGATTGAATTAGGTATTGAGAATTTTGTCTCTCTAAAAGACATTAAGAACAGT AAATGTCCTGAGGGAACAAAACCCATGCTGATATTTGCTGGCGATGATTTCGATGTAACAGAAGATTATA GAAGACTAAAAAGTCTTCTTATTGATTTCTTCAGAGGCCCCACAGTATCAAATATCCGCCTGGCTGGATT AGAGTATGTTCTGCACTTCACTGCACTGAATGGGAAGATTTACTTTCGAAGCTATAAGTTGCTGTTGAAG AAATCTGGTTGCAGAACACCACGGATTGAATTGGAAGAGATGGGACCCTCATTGGATCTGGTTCTGAGGA GGACACACCTGGCATCGGATGACCTTTATAAATTATCTATGAAAATGCCAAAAGCTCTCAAGCCAAAGAA GAAGAAAAATATTTCCCATGATACTTTTGGTACAACTTATGGAAGGATTCATATGCAGAAGCAAGACCTA AGCAAACTACAAACCAGGAAAATGAAGGGGTTGAAGAAGCGACCTGCAGAAAGGATAACAGAAGACCACG AGAAAAAGTCAAAAAGAATTAAAAAAAATTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001289111
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001289111.1, NP_001276040.1
RefSeq Size	3666 bp
RefSeq ORF	732 bp
Locus ID	84154
Gene Summary	Involved in ribosomal large subunit assembly. May regulate the localization of the 5S RNP/5S ribonucleoprotein particle to the nucleolus.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) lacks an alternate 5' exon and initiates translation at an alternate start codon, compared to variant 1. This results in a protein (isoform 2) that has a shorter and distinct N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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