RG9MTD3 (TRMT10B) (NM_001286952) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	bA3J10.9; RG9MTD3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001286952, the custom clone sequence may differ by one or more nucleotides ATGGACTGGAAATTGGAAGGGAGTACTCAGAAAGTAGAGTCACCTGTGCTGCAGGGGCAAGAAGGCATCC TAGAGGAGACAGGTGAAGATGGACTTCCTGAAGGCTTCCAGCTTCTGCAGATCGATGCGGAAGGCGAGTG CCAGGAGGGAGAGATCCTGGCCACAGGCAGTACGGCATGGTGCTCGGAATTAAGTAGACTGGCTGGACAG ATTCGAAGGTTGTATGGTTCAAACAAAAAAGCTGACAGGCCATTTTGGATCTGCCTCACTGGATTCACAA CAGACAGTCCCCTTTATGAAGAGTGTGTGAGGATGAATGATGGATTTTCTAGTTACCTGTTAGACATAAC AGAAGAAGACTGCTTTAGTTTATTTCCCTTGGAAACCCTTGTGTACCTGACTCCTGACTCAGAACACGCT CTTGAAGATGTTGATCTAAACAAAGTTTACATCCTCGGTGGGCTTGTGGATGAAAGCATTCAGAAGAAGG TGACATTTCAAAAGGCCCGGGAATACTCTGTCAAGACCGCACGCTTGCCAATCCAGGAATACATGGTCAG AAACCAGAATGGGAAAAACTATCATTCAGAGATACTGGCCATCAATCAAGAGACTCACAACTGGCCTGAA GCATTGAAGAAAGGAGTTTCTTCAGGAAAAGGCTATATTCTTCGGAACTCAGTGGAATGA
Restriction Sites	SgfI-MluI
ACCN	NM_001286952
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001286952.1, NP_001273881.1
RefSeq Size	2049 bp
RefSeq ORF	690 bp
Locus ID	158234
UniProt ID	Q6PF06
Gene Summary	S-adenosyl-L-methionine-dependent guanine N(1)-methyltransferase that catalyzes the formation of N(1)-methylguanine at position 9 (m1G9) in tRNAs (PubMed:23042678). Probably not able to catalyze formation of N(1)-methyladenine at position 9 (m1A9) in tRNAs (PubMed:23042678).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (4) lacks two alternate exons that result in the loss of an in-frame segment in the 5' coding region, and it uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. The encoded isoform (d) is shorter than isoform a.

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