NCE2 (UBE2F) (NM_001278307) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	NCE2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC333994 representing NM_001278307. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGCTAACGCTAGCAAGTAAACTGAAGCGTGACGATGGTCTCAAAGGGTCCCGGACGGCAGCCACAGCG TCCGACTCGACTCGGAGGGTTTCTGTGAGAGACAAATTGCTTGTTAAAGATGAGGGTTACTACCAGGGT GGAAAATTTCAGTTTGAAACTGAAGTTCCCGATGCGTACAACATGGTGCCTCCCAAAGTGAAATGCCTG ACCAAGATCTGGCACCCCAACATCACAGAGACAGGGGAAATATGTCTGAGTTTATTGAGAGAACATTCA ATTGATGGCACTGGCTGGGCTCCCACAAGAACATTAAAGGATGTCGTTTGGGGATTAAACTCTTTGTTT ACTGATCTTTTGAATTTTGATGATCCACTGAATATTGAAGCTGCAGAACATCATTTGCGGGACAAGGAG GACTTCCGGAATAAAGTGGATGACTACATCAAACGTTATGCCAGATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001278307
Insert Size	462 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001278307.1
RefSeq Size	2110 bp
RefSeq ORF	462 bp
Locus ID	140739
UniProt ID	Q969M7
Protein Pathways	Ubiquitin mediated proteolysis
MW	17.5 kDa
Gene Summary	Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX2, but not RBX1, suggests that the RBX2-UBE2F complex neddylates specific target proteins, such as CUL5.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (4) lacks two alternate exons in the 5' coding region, but maintains the reading frame, compared to variant 1. The encoded isoform (3) is shorter than isoform 1.

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