KPNB1 (NM_001276453) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	IMB1; Impnb; IPO1; IPOB; NTF97
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC333253 representing NM_001276453. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGAAGGAGTCGACATTGGAAGCCATCGGTTATATTTGCCAAGATATAGACCCAGAGCAGCTACAAGAT AAATCCAATGAGATTCTGACTGCCATAATCCAGGGGATGAGGAAAGAAGAGCCTAGTAATAATGTGAAG CTAGCTGCTACGAATGCACTCCTGAACTCATTGGAGTTCACCAAAGCAAACTTTGATAAAGAGTCTGAA AGGCACTTTATTATGCAGGTGGTCTGTGAAGCCACACAGTGTCCAGATACGAGGGTACGAGTGGCTGCT TTACAGAATCTGGTGAAGATAATGTCCTTATATTATCAGTACATGGAGACATATATGGGTCCTGCTCTT TTTGCAATCACAATCGAAGCAATGAAAAGTGACATTGATGAGGTGGCTTTACAAGGGATAGAATTCTGG TCCAATGTCTGTGATGAGGAAATGGATTTGGCCATTGAAGCTTCAGAGGCAGCAGAACAAGGACGGCCC CCTGAGCACACCAGCAAGTTTTATGCGAAGGGAGCACTACAGTATCTGGTTCCAATCCTCACACAGACA CTAACTAAACAGGACGAAAATGATGATGACGATGACTGGAACCCCTGCAAAGCAGCAGGGGTGTGCCTC ATGCTTCTGGCCACCTGCTGTGAAGATGACATTGTCCCACATGTCCTCCCCTTCATTAAAGAACACATC AAGAACCCAGATTGGCGGTACCGGGATGCAGCAGTGATGGCTTTTGGTTGTATCTTGGAAGGACCAGAG CCCAGTCAGCTCAAACCACTAGTTATACAGGCTATGCCCACCCTAATAGAATTAATGAAAGACCCCAGT GTAGTTGTTCGAGATACAGCTGCATGGACTGTAGGCAGAATTTGTGAGCTGCTTCCTGAAGCTGCCATC AATGATGTCTACTTGGCTCCCCTGCTACAGTGTCTGATTGAGGGTCTCAGTGCTGAACCCAGAGTGGCT TCAAATGTGTGCTGGGCTTTCTCCAGTCTGGCTGAAGCTGCTTATGAAGCTGCAGACGTTGCTGATGAT CAGGAAGAACCAGCTACTTACTGCTTATCTTCTTCATTTGAACTCATAGTTCAGAAGCTCCTAGAGACT ACAGACAGACCTGATGGACACCAGAACAACCTGAGGAGTTCTGCATATGAATCTCTGATGGAAATTGTG AAAAACAGTGCCAAGGATTGTTATCCTGCTGTCCAGAAAACGACTTTGGTCATCATGGAACGACTGCAA CAGGTTCTTCAGATGGAGTCACATATCCAGAGCACATCCGATAGAATCCAGTTCAATGACCTTCAGTCT TTACTCTGTGCAACTCTTCAGAATGTTCTTCGGAAAGTGCAACATCAAGATGCTTTGCAGATCTCTGAT GTGGTTATGGCCTCCCTGTTAAGGATGTTCCAAAGCACAGCTGGGTCTGGGGGAGTACAAGAGGATGCC CTGATGGCAGTTAGCACACTGGTGGAAGTGTTGGGTGGTGAATTCCTCAAGTACATGGAGGCCTTTAAA CCCTTCCTGGGCATTGGATTAAAAAATTATGCTGAATACCAGGTTTGTTTGGCAGCTGTGGGCTTAGTG GGAGACTTGTGCCGTGCCCTGCAATCCAACATCATACCTTTCTGTGACGAGGTGATGCAGCTGCTTCTG GAAAATTTGGGGAATGAGAACGTCCACAGGTCTGTGAAGCCGCAGATTCTGTCAGTGTTTGGTGATATT GCCCTTGCTATTGGAGGAGAGTTTAAAAAATACTTAGAGGTTGTATTGAATACTCTTCAGCAGGCCTCC CAAGCCCAGGTGGACAAGTCAGACTATGACATGGTGGATTATCTGAATGAGCTAAGGGAAAGCTGCTTG GAAGCCTATACTGGAATCGTCCAGGGATTAAAGGGGGATCAGGAGAACGTACACCCGGATGTGATGCTG GTACAACCCAGAGTAGAATTTATTCTGTCTTTCATTGACCACATTGCTGGAGATGAGGATCACACAGAT GGAGTAGTAGCTTGTGCTGCTGGACTAATAGGGGACTTATGTACAGCATTTGGGAAGGATGTACTGAAA TTAGTAGAAGCTAGGCCAATGATCCATGAATTGTTAACTGAAGGGCGGAGATCGAAGACTAACAAAGCA AAAACCCTTGCTACATGGGCAACAAAAGAACTGAGGAAACTGAAGAACCAAGCTTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001276453
Insert Size	2196 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001276453.1
RefSeq Size	3951 bp
RefSeq ORF	2196 bp
Locus ID	3837
UniProt ID	Q14974
Protein Families	Druggable Genome, Stem cell - Pluripotency
MW	81.2 kDa
Gene Summary	Nucleocytoplasmic transport, a signal- and energy-dependent process, takes place through nuclear pore complexes embedded in the nuclear envelope. The import of proteins containing a nuclear localization signal (NLS) requires the NLS import receptor, a heterodimer of importin alpha and beta subunits also known as karyopherins. Importin alpha binds the NLS-containing cargo in the cytoplasm and importin beta docks the complex at the cytoplasmic side of the nuclear pore complex. In the presence of nucleoside triphosphates and the small GTP binding protein Ran, the complex moves into the nuclear pore complex and the importin subunits dissociate. Importin alpha enters the nucleoplasm with its passenger protein and importin beta remains at the pore. Interactions between importin beta and the FG repeats of nucleoporins are essential in translocation through the pore complex. The protein encoded by this gene is a member of the importin beta family. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2013] Transcript Variant: This variant (2) has an alternate exon in place of the first two exons compared to variant 1, which causes translation to begin at a downstream AUG compared to variant 1. The resulting isoform (2) is shorter at the N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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