KPNB1 (NM_001276453) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	IMB1; Impnb; IPO1; IPOB; NTF97
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC234789 representing NM_001276453 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGGAGTCGACATTGGAAGCCATCGGTTATATTTGCCAAGATATAGACCCAGAGCAGCTACAAGATA AATCCAATGAGATTCTGACTGCCATAATCCAGGGGATGAGGAAAGAAGAGCCTAGTAATAATGTGAAGCT AGCTGCTACGAATGCACTCCTGAACTCATTGGAGTTCACCAAAGCAAACTTTGATAAAGAGTCTGAAAGG CACTTTATTATGCAGGTGGTCTGTGAAGCCACACAGTGTCCAGATACGAGGGTACGAGTGGCTGCTTTAC AGAATCTGGTGAAGATAATGTCCTTATATTATCAGTACATGGAGACATATATGGGTCCTGCTCTTTTTGC AATCACAATCGAAGCAATGAAAAGTGACATTGATGAGGTGGCTTTACAAGGGATAGAATTCTGGTCCAAT GTCTGTGATGAGGAAATGGATTTGGCCATTGAAGCTTCAGAGGCAGCAGAACAAGGACGGCCCCCTGAGC ACACCAGCAAGTTTTATGCGAAGGGAGCACTACAGTATCTGGTTCCAATCCTCACACAGACACTAACTAA ACAGGACGAAAATGATGATGACGATGACTGGAACCCCTGCAAAGCAGCAGGGGTGTGCCTCATGCTTCTG GCCACCTGCTGTGAAGATGACATTGTCCCACATGTCCTCCCCTTCATTAAAGAACACATCAAGAACCCAG ATTGGCGGTACCGGGATGCAGCAGTGATGGCTTTTGGTTGTATCTTGGAAGGACCAGAGCCCAGTCAGCT CAAACCACTAGTTATACAGGCTATGCCCACCCTAATAGAATTAATGAAAGACCCCAGTGTAGTTGTTCGA GATACAGCTGCATGGACTGTAGGCAGAATTTGTGAGCTGCTTCCTGAAGCTGCCATCAATGATGTCTACT TGGCTCCCCTGCTACAGTGTCTGATTGAGGGTCTCAGTGCTGAACCCAGAGTGGCTTCAAATGTGTGCTG GGCTTTCTCCAGTCTGGCTGAAGCTGCTTATGAAGCTGCAGACGTTGCTGATGATCAGGAAGAACCAGCT ACTTACTGCTTATCTTCTTCATTTGAACTCATAGTTCAGAAGCTCCTAGAGACTACAGACAGACCTGATG GACACCAGAACAACCTGAGGAGTTCTGCATATGAATCTCTGATGGAAATTGTGAAAAACAGTGCCAAGGA TTGTTATCCTGCTGTCCAGAAAACGACTTTGGTCATCATGGAACGACTGCAACAGGTTCTTCAGATGGAG TCACATATCCAGAGCACATCCGATAGAATCCAGTTCAATGACCTTCAGTCTTTACTCTGTGCAACTCTTC AGAATGTTCTTCGGAAAGTGCAACATCAAGATGCTTTGCAGATCTCTGATGTGGTTATGGCCTCCCTGTT AAGGATGTTCCAAAGCACAGCTGGGTCTGGGGGAGTACAAGAGGATGCCCTGATGGCAGTTAGCACACTG GTGGAAGTGTTGGGTGGTGAATTCCTCAAGTACATGGAGGCCTTTAAACCCTTCCTGGGCATTGGATTAA AAAATTATGCTGAATACCAGGTTTGTTTGGCAGCTGTGGGCTTAGTGGGAGACTTGTGCCGTGCCCTGCA ATCCAACATCATACCTTTCTGTGACGAGGTGATGCAGCTGCTTCTGGAAAATTTGGGGAATGAGAACGTC CACAGGTCTGTGAAGCCGCAGATTCTGTCAGTGTTTGGTGATATTGCCCTTGCTATTGGAGGAGAGTTTA AAAAATACTTAGAGGTTGTATTGAATACTCTTCAGCAGGCCTCCCAAGCCCAGGTGGACAAGTCAGACTA TGACATGGTGGATTATCTGAATGAGCTAAGGGAAAGCTGCTTGGAAGCCTATACTGGAATCGTCCAGGGA TTAAAGGGGGATCAGGAGAACGTACACCCGGATGTGATGCTGGTACAACCCAGAGTAGAATTTATTCTGT CTTTCATTGACCACATTGCTGGAGATGAGGATCACACAGATGGAGTAGTAGCTTGTGCTGCTGGACTAAT AGGGGACTTATGTACAGCATTTGGGAAGGATGTACTGAAATTAGTAGAAGCTAGGCCAATGATCCATGAA TTGTTAACTGAAGGGCGGAGATCGAAGACTAACAAAGCAAAAACCCTTGCTACATGGGCAACAAAAGAAC TGAGGAAACTGAAGAACCAAGCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC234789 representing NM_001276453 Red=Cloning site Green=Tags(s) MKESTLEAIGYICQDIDPEQLQDKSNEILTAIIQGMRKEEPSNNVKLAATNALLNSLEFTKANFDKESER HFIMQVVCEATQCPDTRVRVAALQNLVKIMSLYYQYMETYMGPALFAITIEAMKSDIDEVALQGIEFWSN VCDEEMDLAIEASEAAEQGRPPEHTSKFYAKGALQYLVPILTQTLTKQDENDDDDDWNPCKAAGVCLMLL ATCCEDDIVPHVLPFIKEHIKNPDWRYRDAAVMAFGCILEGPEPSQLKPLVIQAMPTLIELMKDPSVVVR DTAAWTVGRICELLPEAAINDVYLAPLLQCLIEGLSAEPRVASNVCWAFSSLAEAAYEAADVADDQEEPA TYCLSSSFELIVQKLLETTDRPDGHQNNLRSSAYESLMEIVKNSAKDCYPAVQKTTLVIMERLQQVLQME SHIQSTSDRIQFNDLQSLLCATLQNVLRKVQHQDALQISDVVMASLLRMFQSTAGSGGVQEDALMAVSTL VEVLGGEFLKYMEAFKPFLGIGLKNYAEYQVCLAAVGLVGDLCRALQSNIIPFCDEVMQLLLENLGNENV HRSVKPQILSVFGDIALAIGGEFKKYLEVVLNTLQQASQAQVDKSDYDMVDYLNELRESCLEAYTGIVQG LKGDQENVHPDVMLVQPRVEFILSFIDHIAGDEDHTDGVVACAAGLIGDLCTAFGKDVLKLVEARPMIHE LLTEGRRSKTNKAKTLATWATKELRKLKNQA TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001276453
ORF Size	2193 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001276453.2
RefSeq Size	3951 bp
RefSeq ORF	2196 bp
Locus ID	3837
UniProt ID	Q14974
Protein Families	Druggable Genome, Stem cell - Pluripotency
MW	81.6 kDa
Gene Summary	Nucleocytoplasmic transport, a signal- and energy-dependent process, takes place through nuclear pore complexes embedded in the nuclear envelope. The import of proteins containing a nuclear localization signal (NLS) requires the NLS import receptor, a heterodimer of importin alpha and beta subunits also known as karyopherins. Importin alpha binds the NLS-containing cargo in the cytoplasm and importin beta docks the complex at the cytoplasmic side of the nuclear pore complex. In the presence of nucleoside triphosphates and the small GTP binding protein Ran, the complex moves into the nuclear pore complex and the importin subunits dissociate. Importin alpha enters the nucleoplasm with its passenger protein and importin beta remains at the pore. Interactions between importin beta and the FG repeats of nucleoporins are essential in translocation through the pore complex. The protein encoded by this gene is a member of the importin beta family. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2013]

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