SEC16A (NM_001276418) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	KIAA0310; p250; SEC16L
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC333251 representing NM_001276418. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGCAGCCACCGCCCCAGACGGTCCCGTCTGGCATGGCTGGGCCACCTCCAGCCGGGAATCCTCGGAGC GTGTTCTGGGCTAGCAGCCCTTACAGGAGACGGGCTAATAATAATGCAGCAGTGGCTCCGACAACTTGC CCGTTGCAGCCGGTCACGGATCCATTTGCTTTTAGTAGACAGGCGCTCCAAAGTACACCACTGGGCAGT TCGTCCAAAAGCAGTCCACCTGTCTTGCAAGGCCCAGCCCCCGCAGGGTTTTCTCAGCACCCCGGTTTG CTTGTTCCTCACACACATGCCAGAGATAGCTCTCAGGGACCCTGTGAGCCCCTGCCTGGACCTCTGACA CAGCCCAGAGCACATGCCAGTCCGTTTTCTGGTGCATTGACACCTTCAGCACCTCCTGGGCCTGAGATG AACAGGAGTGCAGAGGTCGGTCCCAGTTCAGAGCCTGAAGTTCAGACTCTGCCATATCTTCCTCACTAC ATTCCAGGAGTGGATCCTGAAACGTCTCATGGGGGCCACCCTCATGGGAACATGCCTGGGCTCGACCGA CCCCTGAGCAGGCAAAACCCACATGACGGTGTGGTCACCCCAGCAGCATCCCCTTCCCTCCCTCAGCCT GGTCTGCAGATGCCAGGACAGTGGGGGCCAGTGCAGGGAGGCCCACAGCCCTCGGGGCAACATCGTTCA CCCTGCCCTGAAGGACCTGTTCCCAGCGGGGTGCCCTGTGCCACCAGCGTTCCTCATTTCCCCACCCCG TCCATCCTACATCAGGGCCCTGGTCATGAGCAACACAGCCCTCTGGTGGCTCCCCCAGCAGCCTTGCCC AGTGACGGAAGAGACGAGGTGAGCCACTTGCAAAGTGGAAGCCACCTGGCCAATAACTCTGATCCTGAA AGTACATTCAGGCAAAATCCCAGAATTGTGAATCACTGGGCAAGCCCAGAGCTCAGGCAGAATCCAGGA GTGAAGAATGAGCACCGGCCCGCCTCTGCTCTTGTGAACCCCCTCGCCCGGGGAGATAGCCCAGAAAAC CGTACGCACCACCCACTGGGGGCTGGGGCCGGGTCTGGCTGTGCCCCGCTAGAAGCAGACTCAGGAGCT TCAGGAGCTCTGGCGATGTTTTTCCAAGGGGGAGAGACAGAAAATGAGGAGAATCTCTCATCTGAAAAA GCAGGCTTATCTGGTCAAGCGGACTTTGACGATTTCTGCTCCAGCCCTGGGCTAGGCCGTCCGCCCGCA CCTACACACGTGGGGGCAGGCAGCCTCTGCCAGGCCCTTCTCCCAGGCCCCAGCAATGAGGCTGCTGGT GATGTGTGGGGTGACACAGCGAGCACAGGGGTGCCGGATGCCAGCGGCTCGCAGTATGAGAATGTTGAG AACTTAGAATTTGTTCAGAATCAAGAAGTTCTGCCAAGTGAGCCCCTCAATTTGGACCCTTCCTCCCCG AGTGACCAGTTCAGATATGGGCCCCTTCCTGGGCCAGCTGTGCCCAGGCATGGTGCTGTGTGCCACACC GGAGCCCCTGATGCCACACTGCATACAGTGCACCCTGACAGCGTGTCATCCAGCTATAGCAGCAGAAGC CACGGAAGGCTCTCAGGCTCAGCCAGGCCCCAGGAGCTGGTTGGCACATTCATTCAGCAAGAAGTTGGA AAACCCGAGGATGAAGCTTCAGGTAGTTTTTTTAAGCAAATCGATTCTTCTCCCGTAGGAGGTGAAACA GACGAGACCACTGTGAGCCAGAATTACCGTGGCAGCGTGTCCCAGCCCTCAACCCCGAGCCCCCCGAAA CCTACAGGAATATTTCAGACAAGTGCAAATAGTTCTTTCGAACCGGTAAAATCTCACTTAGTTGGGGTA AAACCATTTGAGGCAGATCGCGCCAACGTGGTTGGTGAAGTAAGGGAGACCTGTGTCCGCCAGAAGCAG TGCAGACCAGCTGCCGCCCTGCCCGATGCTTCCCCTGGCAACCTGGAGCAGCCACCAGACAACATGGAG ACCCTCTGTGCACCCCAGGTCTGTCCCCTGCCTCTTAACTCCACCACGGAAGCTGTGCACATGCTTCCG CACGCAGGGGCACCGCCCTTGGATACTGTGTATCCAGCACCCGAGAAGAGGCCTTCAGCCAGGACCCAG GGGCCCGTGAAGTGTGAGAGCCCAGCAACGACTCTGTGGGCGCAAAGTGAGCTGCCAGATTTTGGAGGC AACGTCCTTCTGGCCCCTGCAGCCCCGGCGCTTTATGTGTGTGCAAAACCTCAGCCACCTGTTGTTCAG CCTCCAGAAGAGGCGATGTCCGGGCAGCAGTCACGGAACCCAAGCTCGGCGGCCCCGGTGCAGAGCCGA GGTGGCATTGGTGCTTCTGAGAACCTTGAGAATCCTCCCAAAATGGGAGAGGAGGAGGCCCTTCAGTCC CAGGCGAGTTCTGGTTATGCAAGTTTATTATCCTCACCGCCCACTGAGTCTCTGCAGAATCCTCCAGTC TTGATTGCTCAGCCTGATCACAGCTATAATCTGGCTCAGCCCATTAACTTTTCTGTGTCCTTATCGAAC TCTCATGAGAAGAATCAGTCCTGGAGAGAGGCTTTGGTGGGGGATAGACCTGCAGTCAGCAGTTGGGCT CTCGGTGGTGATTCTGGGGAGAACACTTCTTTGTCTGGGATTCCAACCAGCTCTGTCCTTAGCTTGTCT CTGCCTAGCAGTGTTGCCCAAAGTAATTTTCCACAAGGTTCTGGTGCTTCCGAAATGGTTTCTAATCAG CCTGCTAATTTGCTGGTTCAACCACCATCCCAGCCAGTTCCAGAGAACTTGGTTCCAGAAAGTCAAAAG GATCGTAAGGCAGGAAGTGCTCTTCCCGGATTTGCTAATAGCCCTGCTGGAAGCACAAGTGTGGTGTTA GTTCCACCTGCACACGGCACCCTGGTGCCTGATGGTAATAAGGCAAACCATTCCAGTCATCAGGAAGAC ACTTACGGAGCCCTAGACTTTACCTTAAGCAGGACTTTGGAAAATCCTGTAAACGTGTACAACCCGTCC CATTCTGACAGCCTCGCTTCTCAGCAAAGTGTTGCCAGTCATCCCAGACAATCTGGGCCTGGGGCGCCT AACCTTGACCGTTTTTATCAGCAGGTCACGAAAGATGCCCAGGGCCAGCCTGGCCTCGAAAGAGCCCAG CAGGAGCTGGTGCCACCCCAGCAACAGGCTTCTCCCCCACAACTACCCAAAGCCATGTTTTCGGAGCTG TCAAATCCAGAAAGTCTGCCCGCACAGGGACAGGCCCAGAACTCAGCACAGTCACCAGCAAGTCTGGTT CTGGTCGACGCGGGTCAGCAGCTGCCCCCTCGGCCTCCTCAGTCCTCTAGCGTGTCTCTGGTGTCCAGT GGCTCCGGCCAGGCAGCTGTGCCGTCAGAGCAGCCGTGGCCACAGCCAGTGCCTGCACTTGCCCCCGGC CCACCGCCTCAGGACCTGGCCGCCTACTACTACTACCGGCCTTTGTACGATGCCTACCAGCCTCAGTAC TCTTTGCCGTACCCACCGGAGCCTGGCGCAGCCTCCCTCTATTACCAGGATGTCTACAGCCTCTATGAG CCTCGATACAGGCCCTATGATGGTGCTGCGTCTGCTTACGCCCAGAACTACCGCTATCCCGAGCCCGAG CGGCCCAGCTCCCGAGCCAGCCACTCCTCGGAACGGCCACCTCCCAGGCAAGGATATCCTGAAGGATAC TATAGTTCCAAAAGTGGATGGAGCAGTCAGAGCGATTACTATGCAAGCTATTACTCCAGCCAGTACGAT TATGGAGATCCAGGTCACTGGGATCGTTACCACTACAGTGCTAGAGTCAGGGACCCCCGCACCTATGAC CGGAGGTATTGGTGTGATGCAGAGTATGACGCATACAGGAGAGAGCACTCTGCCTTCGGGGACAGGCCC GAGAAACGTGACAACAACTGGAGGTACGATCCTCGCTTCACGGGGAGTTTTGACGATGACCCCGATCCG CACAGAGACCCTTATGGGGAAGAGGTGGACCGGCGCAGCGTCCACAGCGAGCACTCGGCACGGAGCCTG CACAGCGCACACAGCCTGGCCAGCCGCCGCAGCAGCCTCAGCTCCCACTCGCACCAGAGTCAGATTTAC AGAAGCCACAATGTGGCTGCCGGTTCCTACGAGGCCCCGCTTCCTCCAGGCTCCTTTCACGGCGATTTT GCCTACGGCACCTACCGCAGCAATTTCAGCAGTGGCCCCGGCTTCCCAGAGTATGGCTACCCTGCCGAC ACCGTCTGGCCTGCCATGGAGCAAGTTTCATCAAGACCAACTTCTCCTGAAAAATTTTCAGTGCCTCAT GTCTGTGCCAGGTTTGGCCCTGGCGGTCAGCTTATCAAAGTGATTCCCAATCTGCCTTCAGAAGGACAG CCGGCCTTGGTGGAGGTCCACAGCATGGAGGCCTTGCTGCAGCACACGTCTGAGCAGGAGGAGATGCGG GCGTTCCCGGGACCCCTGGCCAAAGACGACACCCATAAGGTGGATGTCATTAATTTTGCACAGAACAAA GCTATGAAATGTTTGCAGAATGAAAACTTAATTGACAAAGAGTCTGCAAGTCTTCTTTGGAATTTTATT GTTCTCTTATGCAGACAAAATGGGACCGTGGTAGGGACCGACATTGCGGAGCTTCTGTTACGAGACCAC AGAACAGTGTGGCTTCCTGGGAAGTCGCCCAATGAAGCAAACCTGATTGATTTCACGAATGAGGCAGTG GAGCAGGTGGAAGAGGAGGAGTCTGGTGAGGCCCAGCTCTCTTTCCTCACTGGTGGTCCGGCGGCTGCC GCCAGCTCGCTCGAGAGAGAGACCGAGAGGTTCAGGGAGCTGTTGCTGTATGGCCGTAAGAAGGATGCT TTGGAGTCTGCAATGAAGAATGGCCTGTGGGGTCACGCTCTGCTACTTGCAAGTAAGATGGACAGCCGG ACACACGCCCGAGTCATGACCAGGTTTGCTAACAGCCTCCCAATCAACGACCCTCTGCAGACAGTCTAC CAGCTCATGTCCGGACGGATGCCTGCCGCGTCCACGTGCTGTGGAGACGAGAAATGGGGAGATTGGAGG CCGCACCTCGCCATGGTCTTGTCCAACTTGAACAACAACATGGACGTCGAGTCCAGGACGATGGCTACC ATGGGCGACACTCTGGCTTCAAGGGGCCTCTTGGATGCGGCCCACTTCTGCTACCTCATGGCCCAGGCG GGATTTGGTGTTTACACGAAGAAAACTACAAAGCTTGTCTTAATCGGATCCAATCACAGTTTGCCATTC TTAAAGTTCGCAACCAACGAAGCAATCCAGAGGACGGAAGCCTATGAGTACGCCCAGTCCCTGGGTGCC GAGACCTGCCCCCTGCCTAGTTTCCAGGTGTTTAAGTTCATCTACTCCTGCCGCCTGGCGGAAATGGGG CTGGCCACGCAAGCCTTCCACTACTGTGAGGCCATCGCGAAGAGCATCCTGACGCAGCCGCACCTGTAT TCCCCGGTGTTGATCAGCCAGCTTGTGCAGATGGCTTCCCAGTTACGACTCTTCGATCCCCAGCTGAAA GAGAAGCCAGAAGAGGAGTCCTTGGCCGCACCCACGTGGCTGGTTCACCTGCAGCAGGTGGAGCGGCAG ATTAAGGAGGGGGCTGGAGTATGGCATCAGGATGGAGCCCTCCCGCAGCAGTGTCCTGGCACTCCGAGT TCCGAGATGGAGCAGTTGGACAGGCCAGGACTCAGTCAGCCAGGAGCCCTGGGGATCGCCAACCCTCTG CTGGCGGTGCCTGCACCGAGCCCTGAGCACTCGAGCCCGAGCGTGCGGCTGCTGCCCTCAGCTCCGCAG ACGCTCCCTGACGGCCCATTGGCCAGTCCTGCCAGAGTGCCGATGTTCCCAGTGCCACTGCCCCCGGGG CCCCTGGAGCCGGGTCCTGGCTGTGTGACCCCAGGGCCTGCACTTGGCTTCCTGGAGCCCTCCGGGCCT GGCCTCCCACCTGGTGTGCCACCTCTGCAGGAAAGGAGACACTTGCTCCAGGAAGCCAGGAGCCCAGAC CCAGGGATAGTGCCGCAGGAGGCGCCTGTTGGAAACTCACTTTCCGAGCTAAGCGAAGAAAATTTTGAT GGAAAATTTGCTAATCTGACCCCCTCGAGGACGGTGCCAGACTCGGAGGCCCCCCCAGGGTGGGATCGT GCCGACTCGGGTCCCACGCAGCCACCTCTGTCTCTCTCACCCGCTCCCGAAACAAAGAGACCCGGACAG GCAGCCAAGAAAGAAACGAAGGAACCTAAGAAGGGTGAATCCTGGTTCTTTCGTTGGCTACCTGGAAAG AAAAAGACAGAAGCTTATTTGCCAGATGACAAGAACAAATCGATTGTTTGGGATGAAAAGAAAAACCAG TGGGTGAATTTAAATGAGCCAGAAGAGGAGAAGAAAGCCCCGCCCCCACCTCCAACCTCGATGCCCAAG ACTGTGCAAGCTGCCCCGCCTGCCCTCCCAGGGCCTCCTGGAGCCCCCGTGAACATGTACTCTAGAAGA GCAGCAGGAACCAGAGCTCGCTACGTTGACGTCCTGAACCCAAGCGGGACCCAGCGGAGCGAGCCGGCT CTCGCTCCTGCGGACTTTGTCGCTCCACTCGCGCCACTCCCAATTCCTTCTAACTTGTTCGTGCCAACC CCAGTGTCTTCAGTGAGACCCCAGGGACGTTCGGGACGGAATGATGGCCTCCTCGCTCTCTCATCCCCA GATGCAGAAGAACCACAGCTTCCAGACGGGACTGGCAGGGAAGGGCCTGCAGCAGCTAGGGGCCTGGCC AATCCAGAGCCTGCCCCAGAGCCCAAGGCTCCTGGCGACCTCCCTGCTGCAGGGGGCCCTCCCAGCGGG GCCATGCCCTTCTACAACCCTGCTCAGCTGGCACAGGCCTGCGCCACCTCCGGGAGCTCAAGGCTAGGG AGGATTGGCCAGAGGAAGCACCTGGTGCTGAACTAG
Restriction Sites	SgfI-MluI
ACCN	NM_001276418
Insert Size	7005 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001276418.1
RefSeq Size	9000 bp
RefSeq ORF	7005 bp
Locus ID	9919
UniProt ID	O15027
MW	249.5 kDa
Gene Summary	This gene encodes a protein that forms part of the Sec16 complex. This protein has a role in protein transport from the endoplasmic reticulum (ER) to the Golgi and mediates COPII vesicle formation at the transitional ER. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Feb 2013] Transcript Variant: This variant (2) differs in the 5' UTR and lacks two alternate in-frame exons in the coding region compared to variant 1. It encodes isoform 2 which is shorter compared to isoform 1.

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