SEC16A (NM_001276418) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	KIAA0310; p250; SEC16L
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC235387 representing NM_001276418 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGCCACCGCCCCAGACGGTCCCGTCTGGCATGGCTGGGCCACCTCCAGCCGGGAATCCTCGGAGCG TGTTCTGGGCTAGCAGCCCTTACAGGAGACGGGCTAATAATAATGCAGCAGTGGCTCCGACAACTTGCCC GTTGCAGCCGGTCACGGATCCATTTGCTTTTAGTAGACAGGCGCTCCAAAGTACACCACTGGGCAGTTCG TCCAAAAGCAGTCCACCTGTCTTGCAAGGCCCAGCCCCCGCAGGGTTTTCTCAGCACCCCGGTTTGCTTG TTCCTCACACACATGCCAGAGATAGCTCTCAGGGACCCTGTGAGCCCCTGCCTGGACCTCTGACACAGCC CAGAGCACATGCCAGTCCGTTTTCTGGTGCATTGACACCTTCAGCACCTCCTGGGCCTGAGATGAACAGG AGTGCAGAGGTCGGTCCCAGTTCAGAGCCTGAAGTTCAGACTCTGCCATATCTTCCTCACTACATTCCAG GAGTGGATCCTGAAACGTCTCATGGGGGCCACCCTCATGGGAACATGCCTGGGCTCGACCGACCCCTGAG CAGGCAAAACCCACATGACGGTGTGGTCACCCCAGCAGCATCCCCTTCCCTCCCTCAGCCTGGTCTGCAG ATGCCAGGACAGTGGGGGCCAGTGCAGGGAGGCCCACAGCCCTCGGGGCAACATCGTTCACCCTGCCCTG AAGGACCTGTTCCCAGCGGGGTGCCCTGTGCCACCAGCGTTCCTCATTTCCCCACCCCGTCCATCCTACA TCAGGGCCCTGGTCATGAGCAACACAGCCCTCTGGTGGCTCCCCCAGCAGCCTTGCCCAGTGACGGAAGA GACGAGGTGAGCCACTTGCAAAGTGGAAGCCACCTGGCCAATAACTCTGATCCTGAAAGTACATTCAGGC AAAATCCCAGAATTGTGAATCACTGGGCAAGCCCAGAGCTCAGGCAGAATCCAGGAGTGAAGAATGAGCA CCGGCCCGCCTCTGCTCTTGTGAACCCCCTCGCCCGGGGAGATAGCCCAGAAAACCGTACGCACCACCCA CTGGGGGCTGGGGCCGGGTCTGGCTGTGCCCCGCTAGAAGCAGACTCAGGAGCTTCAGGAGCTCTGGCGA TGTTTTTCCAAGGGGGAGAGACAGAAAATGAGGAGAATCTCTCATCTGAAAAAGCAGGCTTATCTGGTCA AGCGGACTTTGACGATTTCTGCTCCAGCCCTGGGCTAGGCCGTCCGCCCGCACCTACACACGTGGGGGCA GGCAGCCTCTGCCAGGCCCTTCTCCCAGGCCCCAGCAATGAGGCTGCTGGTGATGTGTGGGGTGACACAG CGAGCACAGGGGTGCCGGATGCCAGCGGCTCGCAGTATGAGAATGTTGAGAACTTAGAATTTGTTCAGAA TCAAGAAGTTCTGCCAAGTGAGCCCCTCAATTTGGACCCTTCCTCCCCGAGTGACCAGTTCAGATATGGG CCCCTTCCTGGGCCAGCTGTGCCCAGGCATGGTGCTGTGTGCCACACCGGAGCCCCTGATGCCACACTGC ATACAGTGCACCCTGACAGCGTGTCATCCAGCTATAGCAGCAGAAGCCACGGAAGGCTCTCAGGCTCAGC CAGGCCCCAGGAGCTGGTTGGCACATTCATTCAGCAAGAAGTTGGAAAACCCGAGGATGAAGCTTCAGGT AGTTTTTTTAAGCAAATCGATTCTTCTCCCGTAGGAGGTGAAACAGACGAGACCACTGTGAGCCAGAATT ACCGTGGCAGCGTGTCCCAGCCCTCAACCCCGAGCCCCCCGAAACCTACAGGAATATTTCAGACAAGTGC AAATAGTTCTTTCGAACCGGTAAAATCTCACTTAGTTGGGGTAAAACCATTTGAGGCAGATCGCGCCAAC GTGGTTGGTGAAGTAAGGGAGACCTGTGTCCGCCAGAAGCAGTGCAGACCAGCTGCCGCCCTGCCCGATG CTTCCCCTGGCAACCTGGAGCAGCCACCAGACAACATGGAGACCCTCTGTGCACCCCAGGTCTGTCCCCT GCCTCTTAACTCCACCACGGAAGCTGTGCACATGCTTCCGCACGCAGGGGCACCGCCCTTGGATACTGTG TATCCAGCACCCGAGAAGAGGCCTTCAGCCAGGACCCAGGGGCCCGTGAAGTGTGAGAGCCCAGCAACGA CTCTGTGGGCGCAAAGTGAGCTGCCAGATTTTGGAGGCAACGTCCTTCTGGCCCCTGCAGCCCCGGCGCT TTATGTGTGTGCAAAACCTCAGCCACCTGTTGTTCAGCCTCCAGAAGAGGCGATGTCCGGGCAGCAGTCA CGGAACCCAAGCTCGGCGGCCCCGGTGCAGAGCCGAGGTGGCATTGGTGCTTCTGAGAACCTTGAGAATC CTCCCAAAATGGGAGAGGAGGAGGCCCTTCAGTCCCAGGCGAGTTCTGGTTATGCAAGTTTATTATCCTC ACCGCCCACTGAGTCTCTGCAGAATCCTCCAGTCTTGATTGCTCAGCCTGATCACAGCTATAATCTGGCT CAGCCCATTAACTTTTCTGTGTCCTTATCGAACTCTCATGAGAAGAATCAGTCCTGGAGAGAGGCTTTGG TGGGGGATAGACCTGCAGTCAGCAGTTGGGCTCTCGGTGGTGATTCTGGGGAGAACACTTCTTTGTCTGG GATTCCAACCAGCTCTGTCCTTAGCTTGTCTCTGCCTAGCAGTGTTGCCCAAAGTAATTTTCCACAAGGT TCTGGTGCTTCCGAAATGGTTTCTAATCAGCCTGCTAATTTGCTGGTTCAACCACCATCCCAGCCAGTTC CAGAGAACTTGGTTCCAGAAAGTCAAAAGGATCGTAAGGCAGGAAGTGCTCTTCCCGGATTTGCTAATAG CCCTGCTGGAAGCACAAGTGTGGTGTTAGTTCCACCTGCACACGGCACCCTGGTGCCTGATGGTAATAAG GCAAACCATTCCAGTCATCAGGAAGACACTTACGGAGCCCTAGACTTTACCTTAAGCAGGACTTTGGAAA ATCCTGTAAACGTGTACAACCCGTCCCATTCTGACAGCCTCGCTTCTCAGCAAAGTGTTGCCAGTCATCC CAGACAATCTGGGCCTGGGGCGCCTAACCTTGACCGTTTTTATCAGCAGGTCACGAAAGATGCCCAGGGC CAGCCTGGCCTCGAAAGAGCCCAGCAGGAGCTGGTGCCACCCCAGCAACAGGCTTCTCCCCCACAACTAC CCAAAGCCATGTTTTCGGAGCTGTCAAATCCAGAAAGTCTGCCCGCACAGGGACAGGCCCAGAACTCAGC ACAGTCACCAGCAAGTCTGGTTCTGGTCGACGCGGGTCAGCAGCTGCCCCCTCGGCCTCCTCAGTCCTCT AGCGTGTCTCTGGTGTCCAGTGGCTCCGGCCAGGCAGCTGTGCCGTCAGAGCAGCCGTGGCCACAGCCAG TGCCTGCACTTGCCCCCGGCCCACCGCCTCAGGACCTGGCCGCCTACTACTACTACCGGCCTTTGTACGA TGCCTACCAGCCTCAGTACTCTTTGCCGTACCCACCGGAGCCTGGCGCAGCCTCCCTCTATTACCAGGAT GTCTACAGCCTCTATGAGCCTCGATACAGGCCCTATGATGGTGCTGCGTCTGCTTACGCCCAGAACTACC GCTATCCCGAGCCCGAGCGGCCCAGCTCCCGAGCCAGCCACTCCTCGGAACGGCCACCTCCCAGGCAAGG ATATCCTGAAGGATACTATAGTTCCAAAAGTGGATGGAGCAGTCAGAGCGATTACTATGCAAGCTATTAC TCCAGCCAGTACGATTATGGAGATCCAGGTCACTGGGATCGTTACCACTACAGTGCTAGAGTCAGGGACC CCCGCACCTATGACCGGAGGTATTGGTGTGATGCAGAGTATGACGCATACAGGAGAGAGCACTCTGCCTT CGGGGACAGGCCCGAGAAACGTGACAACAACTGGAGGTACGATCCTCGCTTCACGGGGAGTTTTGACGAT GACCCCGATCCGCACAGAGACCCTTATGGGGAAGAGGTGGACCGGCGCAGCGTCCACAGCGAGCACTCGG CACGGAGCCTGCACAGCGCACACAGCCTGGCCAGCCGCCGCAGCAGCCTCAGCTCCCACTCGCACCAGAG TCAGATTTACAGAAGCCACAATGTGGCTGCCGGTTCCTACGAGGCCCCGCTTCCTCCAGGCTCCTTTCAC GGCGATTTTGCCTACGGCACCTACCGCAGCAATTTCAGCAGTGGCCCCGGCTTCCCAGAGTATGGCTACC CTGCCGACACCGTCTGGCCTGCCATGGAGCAAGTTTCATCAAGACCAACTTCTCCTGAAAAATTTTCAGT GCCTCATGTCTGTGCCAGGTTTGGCCCTGGCGGTCAGCTTATCAAAGTGATTCCCAATCTGCCTTCAGAA GGACAGCCGGCCTTGGTGGAGGTCCACAGCATGGAGGCCTTGCTGCAGCACACGTCTGAGCAGGAGGAGA TGCGGGCGTTCCCGGGACCCCTGGCCAAAGACGACACCCATAAGGTGGATGTCATTAATTTTGCACAGAA CAAAGCTATGAAATGTTTGCAGAATGAAAACTTAATTGACAAAGAGTCTGCAAGTCTTCTTTGGAATTTT ATTGTTCTCTTATGCAGACAAAATGGGACCGTGGTAGGGACCGACATTGCGGAGCTTCTGTTACGAGACC ACAGAACAGTGTGGCTTCCTGGGAAGTCGCCCAATGAAGCAAACCTGATTGATTTCACGAATGAGGCAGT GGAGCAGGTGGAAGAGGAGGAGTCTGGTGAGGCCCAGCTCTCTTTCCTCACTGGTGGTCCGGCGGCTGCC GCCAGCTCGCTCGAGAGAGAGACCGAGAGGTTCAGGGAGCTGTTGCTGTATGGCCGTAAGAAGGATGCTT TGGAGTCTGCAATGAAGAATGGCCTGTGGGGTCACGCTCTGCTACTTGCAAGTAAGATGGACAGCCGGAC ACACGCCCGAGTCATGACCAGGTTTGCTAACAGCCTCCCAATCAACGACCCTCTGCAGACAGTCTACCAG CTCATGTCCGGACGGATGCCTGCCGCGTCCACGTGCTGTGGAGACGAGAAATGGGGAGATTGGAGGCCGC ACCTCGCCATGGTCTTGTCCAACTTGAACAACAACATGGACGTCGAGTCCAGGACGATGGCTACCATGGG CGACACTCTGGCTTCAAGGGGCCTCTTGGATGCGGCCCACTTCTGCTACCTCATGGCCCAGGCGGGATTT GGTGTTTACACGAAGAAAACTACAAAGCTTGTCTTAATCGGATCCAATCACAGTTTGCCATTCTTAAAGT TCGCAACCAACGAAGCAATCCAGAGGACGGAAGCCTATGAGTACGCCCAGTCCCTGGGTGCCGAGACCTG CCCCCTGCCTAGTTTCCAGGTGTTTAAGTTCATCTACTCCTGCCGCCTGGCGGAAATGGGGCTGGCCACG CAAGCCTTCCACTACTGTGAGGCCATCGCGAAGAGCATCCTGACGCAGCCGCACCTGTATTCCCCGGTGT TGATCAGCCAGCTTGTGCAGATGGCTTCCCAGTTACGACTCTTCGATCCCCAGCTGAAAGAGAAGCCAGA AGAGGAGTCCTTGGCCGCACCCACGTGGCTGGTTCACCTGCAGCAGGTGGAGCGGCAGATTAAGGAGGGG GCTGGAGTATGGCATCAGGATGGAGCCCTCCCGCAGCAGTGTCCTGGCACTCCGAGTTCCGAGATGGAGC AGTTGGACAGGCCAGGACTCAGTCAGCCAGGAGCCCTGGGGATCGCCAACCCTCTGCTGGCGGTGCCTGC ACCGAGCCCTGAGCACTCGAGCCCGAGCGTGCGGCTGCTGCCCTCAGCTCCGCAGACGCTCCCTGACGGC CCATTGGCCAGTCCTGCCAGAGTGCCGATGTTCCCAGTGCCACTGCCCCCGGGGCCCCTGGAGCCGGGTC CTGGCTGTGTGACCCCAGGGCCTGCACTTGGCTTCCTGGAGCCCTCCGGGCCTGGCCTCCCACCTGGTGT GCCACCTCTGCAGGAAAGGAGACACTTGCTCCAGGAAGCCAGGAGCCCAGACCCAGGGATAGTGCCGCAG GAGGCGCCTGTTGGAAACTCACTTTCCGAGCTAAGCGAAGAAAATTTTGATGGAAAATTTGCTAATCTGA CCCCCTCGAGGACGGTGCCAGACTCGGAGGCCCCCCCAGGGTGGGATCGTGCCGACTCGGGTCCCACGCA GCCACCTCTGTCTCTCTCACCCGCTCCCGAAACAAAGAGACCCGGACAGGCAGCCAAGAAAGAAACGAAG GAACCTAAGAAGGGTGAATCCTGGTTCTTTCGTTGGCTACCTGGAAAGAAAAAGACAGAAGCTTATTTGC CAGATGACAAGAACAAATCGATTGTTTGGGATGAAAAGAAAAACCAGTGGGTGAATTTAAATGAGCCAGA AGAGGAGAAGAAAGCCCCGCCCCCACCTCCAACCTCGATGCCCAAGACTGTGCAAGCTGCCCCGCCTGCC CTCCCAGGGCCTCCTGGAGCCCCCGTGAACATGTACTCTAGAAGAGCAGCAGGAACCAGAGCTCGCTACG TTGACGTCCTGAACCCAAGCGGGACCCAGCGGAGCGAGCCGGCTCTCGCTCCTGCGGACTTTGTCGCTCC ACTCGCGCCACTCCCAATTCCTTCTAACTTGTTCGTGCCAACCCCAGTGTCTTCAGTGAGACCCCAGGGA CGTTCGGGACGGAATGATGGCCTCCTCGCTCTCTCATCCCCAGATGCAGAAGAACCACAGCTTCCAGACG GGACTGGCAGGGAAGGGCCTGCAGCAGCTAGGGGCCTGGCCAATCCAGAGCCTGCCCCAGAGCCCAAGGC TCCTGGCGACCTCCCTGCTGCAGGGGGCCCTCCCAGCGGGGCCATGCCCTTCTACAACCCTGCTCAGCTG GCACAGGCCTGCGCCACCTCCGGGAGCTCAAGGCTAGGGAGGATTGGCCAGAGGAAGCACCTGGTGCTGA AC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC235387 representing NM_001276418 Red=Cloning site Green=Tags(s) MQPPPQTVPSGMAGPPPAGNPRSVFWASSPYRRRANNNAAVAPTTCPLQPVTDPFAFSRQALQSTPLGSS SKSSPPVLQGPAPAGFSQHPGLLVPHTHARDSSQGPCEPLPGPLTQPRAHASPFSGALTPSAPPGPEMNR SAEVGPSSEPEVQTLPYLPHYIPGVDPETSHGGHPHGNMPGLDRPLSRQNPHDGVVTPAASPSLPQPGLQ MPGQWGPVQGGPQPSGQHRSPCPEGPVPSGVPCATSVPHFPTPSILHQGPGHEQHSPLVAPPAALPSDGR DEVSHLQSGSHLANNSDPESTFRQNPRIVNHWASPELRQNPGVKNEHRPASALVNPLARGDSPENRTHHP LGAGAGSGCAPLEADSGASGALAMFFQGGETENEENLSSEKAGLSGQADFDDFCSSPGLGRPPAPTHVGA GSLCQALLPGPSNEAAGDVWGDTASTGVPDASGSQYENVENLEFVQNQEVLPSEPLNLDPSSPSDQFRYG PLPGPAVPRHGAVCHTGAPDATLHTVHPDSVSSSYSSRSHGRLSGSARPQELVGTFIQQEVGKPEDEASG SFFKQIDSSPVGGETDETTVSQNYRGSVSQPSTPSPPKPTGIFQTSANSSFEPVKSHLVGVKPFEADRAN VVGEVRETCVRQKQCRPAAALPDASPGNLEQPPDNMETLCAPQVCPLPLNSTTEAVHMLPHAGAPPLDTV YPAPEKRPSARTQGPVKCESPATTLWAQSELPDFGGNVLLAPAAPALYVCAKPQPPVVQPPEEAMSGQQS RNPSSAAPVQSRGGIGASENLENPPKMGEEEALQSQASSGYASLLSSPPTESLQNPPVLIAQPDHSYNLA QPINFSVSLSNSHEKNQSWREALVGDRPAVSSWALGGDSGENTSLSGIPTSSVLSLSLPSSVAQSNFPQG SGASEMVSNQPANLLVQPPSQPVPENLVPESQKDRKAGSALPGFANSPAGSTSVVLVPPAHGTLVPDGNK ANHSSHQEDTYGALDFTLSRTLENPVNVYNPSHSDSLASQQSVASHPRQSGPGAPNLDRFYQQVTKDAQG QPGLERAQQELVPPQQQASPPQLPKAMFSELSNPESLPAQGQAQNSAQSPASLVLVDAGQQLPPRPPQSS SVSLVSSGSGQAAVPSEQPWPQPVPALAPGPPPQDLAAYYYYRPLYDAYQPQYSLPYPPEPGAASLYYQD VYSLYEPRYRPYDGAASAYAQNYRYPEPERPSSRASHSSERPPPRQGYPEGYYSSKSGWSSQSDYYASYY SSQYDYGDPGHWDRYHYSARVRDPRTYDRRYWCDAEYDAYRREHSAFGDRPEKRDNNWRYDPRFTGSFDD DPDPHRDPYGEEVDRRSVHSEHSARSLHSAHSLASRRSSLSSHSHQSQIYRSHNVAAGSYEAPLPPGSFH GDFAYGTYRSNFSSGPGFPEYGYPADTVWPAMEQVSSRPTSPEKFSVPHVCARFGPGGQLIKVIPNLPSE GQPALVEVHSMEALLQHTSEQEEMRAFPGPLAKDDTHKVDVINFAQNKAMKCLQNENLIDKESASLLWNF IVLLCRQNGTVVGTDIAELLLRDHRTVWLPGKSPNEANLIDFTNEAVEQVEEEESGEAQLSFLTGGPAAA ASSLERETERFRELLLYGRKKDALESAMKNGLWGHALLLASKMDSRTHARVMTRFANSLPINDPLQTVYQ LMSGRMPAASTCCGDEKWGDWRPHLAMVLSNLNNNMDVESRTMATMGDTLASRGLLDAAHFCYLMAQAGF GVYTKKTTKLVLIGSNHSLPFLKFATNEAIQRTEAYEYAQSLGAETCPLPSFQVFKFIYSCRLAEMGLAT QAFHYCEAIAKSILTQPHLYSPVLISQLVQMASQLRLFDPQLKEKPEEESLAAPTWLVHLQQVERQIKEG AGVWHQDGALPQQCPGTPSSEMEQLDRPGLSQPGALGIANPLLAVPAPSPEHSSPSVRLLPSAPQTLPDG PLASPARVPMFPVPLPPGPLEPGPGCVTPGPALGFLEPSGPGLPPGVPPLQERRHLLQEARSPDPGIVPQ EAPVGNSLSELSEENFDGKFANLTPSRTVPDSEAPPGWDRADSGPTQPPLSLSPAPETKRPGQAAKKETK EPKKGESWFFRWLPGKKKTEAYLPDDKNKSIVWDEKKNQWVNLNEPEEEKKAPPPPPTSMPKTVQAAPPA LPGPPGAPVNMYSRRAAGTRARYVDVLNPSGTQRSEPALAPADFVAPLAPLPIPSNLFVPTPVSSVRPQG RSGRNDGLLALSSPDAEEPQLPDGTGREGPAAARGLANPEPAPEPKAPGDLPAAGGPPSGAMPFYNPAQL AQACATSGSSRLGRIGQRKHLVLN TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001276418
ORF Size	7002 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001276418.1, NP_001263347.1
RefSeq Size	9000 bp
RefSeq ORF	7005 bp
Locus ID	9919
UniProt ID	O15027
MW	249.9 kDa
Gene Summary	This gene encodes a protein that forms part of the Sec16 complex. This protein has a role in protein transport from the endoplasmic reticulum (ER) to the Golgi and mediates COPII vesicle formation at the transitional ER. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Feb 2013]

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