CHST15 (NM_001270765) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	BRAG; GALNAC4S-6ST
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332999 representing NM_001270765. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGAGGCACTGCATTAATTGCTGCATACAGCTGTTACCCGACGGCGCACACAAGCAGCAGGTCAACTGC CAAGGGGGCCCCCATCACGGTCACCAGGCGTGCCCCACGTGCAAAGGAGAAAACAAAATTCTGTTTCGT GTGGACAGTAAGCAGATGAACTTGCTTGCTGTTCTCGAAGTGAGGACTGAAGGGAACGAAAACTGGGGT GGGTTTTTGCGCTTCAAAAAGGGGAAGCGATGTAGCCTCGTTTTTGGACTGATAATAATGACCTTGGTA ATGGCTTCTTACATCCTTTCTGGGGCCCACCAAGAGCTTCTGATCTCATCACCTTTCCATTACGGAGGC TTCCCCAGCAACCCCAGCTTGATGGACAGCGAAAACCCAAGTGACACAAAGGAGCATCACCACCAATCC TCTGTAAATAATATTTCATACATGAAGGACTATCCAAGCATTAAATTAATTATCAACAGCATCACAACT AGGATTGAGTTCACGACCAGACAGCTCCCAGACTTAGAAGACCTTAAGAAGCAGGAGTTGCATATGTTT TCAGTCATCCCCAACAAATTCCTTCCAAACAGTAAGAGCCCCTGTTGGTACGAGGAGTTCTCGGGGCAG AACACCACCGACCCCTACCTCACCAACTCCTACGTGCTCTACTCCAAGCGCTTCCGCTCCACCTTCGAC GCCCTGCGCAAGGCCTTCTGGGGCCACCTGGCGCACGCGCACGGGAAGCACTTCCGCCTGCGCTGCCTG CCGCACTTCTACATCATAGGGCAGCCCAAGTGCGGGACCACAGACCTCTATGACCGCCTGCGGCTGCAC CCTGAGGTCAAGTTCTCCGCCATCAAGGAGCCACACTGGTGGACCCGGAAGCGCTTTGGAATCGTCCGC CTAAGAGATGGGCTGCGAGACCGCTATCCCGTGGAAGATTATCTGGACCTCTTTGACCTGGCCGCACAC CAGATCCATCAAGGACTGCAGGCCAGCTCTGCAAAGGAGCAGAGCAAGATGAATACAATCATTATCGGG GAGGCCAGTGCCTCCACGATGTGGGATAATAATGCCTGGACGTTCTTCTACGACAACAGCACGGATGGC GAGCCACCGTTTCTGACGCAGGACTTCATCCACGCCTTTCAGCCAAATGCCAGACTGATTGTCATGCTC AGGGACCCTGTGGAGAGGTTGTACTCAGACTATCTCTACTTTGCAAGTTCGAATAAATCCGCGGACGAC TTCCATGAGAAAGTGACAGAAGCACTGCAGCTGTTTGAAAATTGCATGCTTGATTATTCACTGCGCGCC TGCGTCTACAACAACACCCTCAACAACGCCATGCCTGTGTGTACCCCCCCCCCCCGTACCCCCCGAGCT GGCCCCTGGCAGAAGGAGCTGGTTTGTTGTTATTATGCAAGCGGCATTGTGGGTTTGCGTTTCAGCATA GGAACAGAGAGAAGCGTTTTAATGTGCAAATGCTGTTCCCCATTATTCATGGATGTAAAAGCTGAAAAC TGA
Restriction Sites	SgfI-MluI
ACCN	NM_001270765
Insert Size	1521 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001270765.1
RefSeq Size	3618 bp
RefSeq ORF	1521 bp
Locus ID	51363
UniProt ID	Q7LFX5
Protein Families	Transmembrane
Protein Pathways	Chondroitin sulfate biosynthesis
MW	58.1 kDa
Gene Summary	Chondroitin sulfate (CS) is a glycosaminoglycan which is an important structural component of the extracellular matrix and which links to proteins to form proteoglycans. Chondroitin sulfate E (CS-E) is an isomer of chondroitin sulfate in which the C-4 and C-6 hydroxyl groups are sulfated. This gene encodes a type II transmembrane glycoprotein that acts as a sulfotransferase to transfer sulfate to the C-6 hydroxal group of chondroitin sulfate. This gene has also been identified as being co-expressed with RAG1 in B-cells and as potentially acting as a B-cell surface signaling receptor. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2012] Transcript Variant: This variant (4) differs in the 5' UTR, 3' UTR, and 3' coding sequence compared to variant 1. The resulting isoform (2) has a shorter and distinct C-terminus compared to isoform 1. Variants 2 and 4 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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