CAMKK2 (NM_001270486) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CAMKK; CAMKKB
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC332957 representing NM_001270486. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGTCATCATGTGTCTCTAGCCAGCCCAGCAGCAACCGGGCCGCCCCCCAGGATGAGCTGGGGGGCAGG GGCAGCAGCAGCAGCGAAAGCCAGAAGCCCTGTGAGGCCCTGCGGGGCCTCTCATCCTTGAGCATCCAC CTGGGCATGGAGTCCTTCATTGTGGTCACCGAGTGTGAGCCGGGCTGTGCTGTGGACCTCGGCTTGGCG CGGGACCGGCCCCTGGAGGCCGATGGCCAAGAGGTCCCCCTTGACACCTCCGGGTCCCAGGCCCGGCCC CACCTCTCCGGTCGCAAGCTGTCTCTGCAAGAGCGGTCCCAGGGTGGGCTGGCAGCCGGTGGCAGCCTG GACATGAACGGACGCTGCATCTGCCCGTCCCTGCCCTACTCACCCGTCAGCTCCCCGCAGTCCTCGCCT CGGCTGCCCCGGCGGCCGACAGTGGAGTCTCACCACGTCTCCATCACGGGTATGCAGGACTGTGTGCAG CTGAATCAGTATACCCTGAAGGATGAAATTGGAAAGGGCTCCTATGGTGTCGTCAAGTTGGCCTACAAT GAAAATGACAATACCTACTATGCAATGAAGGTGCTGTCCAAAAAGAAGCTGATCCGGCAGGCCGGCTTT CCACGTCGCCCTCCACCCCGAGGCACCCGGCCAGCTCCTGGAGGCTGCATCCAGCCCAGGGGCCCCATT GAGCAGGTGTACCAGGAAATTGCCATCCTCAAGAAGCTGGACCACCCCAATGTGGTGAAGCTGGTGGAG GTCCTGGATGACCCCAATGAGGACCATCTGTACATGGTGTTCGAACTGGTCAACCAAGGGCCCGTGATG GAAGTGCCCACCCTCAAACCACTCTCTGAAGACCAGGCCCGTTTCTACTTCCAGGATCTGATCAAAGGC ATCGAGTACTTACACTACCAGAAGATCATCCACCGTGACATCAAACCTTCCAACCTCCTGGTCGGAGAA GATGGGCACATCAAGATCGCTGACTTTGGTGTGAGCAATGAATTCAAGGGCAGTGACGCGCTCCTCTCC AACACCGTGGGCACGCCCGCCTTCATGGCACCCGAGTCGCTCTCTGAGACCCGCAAGATCTTCTCTGGG AAGGCCTTGGATGTTTGGGCCATGGGTGTGACACTATACTGCTTTGTCTTTGGCCAGTGCCCATTCATG GACGAGCGGATCATGTGTTTACACAGTAAGATCAAGAGTCAGGCCCTGGAATTTCCAGACCAGCCCGAC ATAGCTGAGGACTTGAAGGACCTGATCACCCGTATGCTGGACAAGAACCCCGAGTCGAGGATCGTGGTG CCGGAAATCAAGCTGCACCCCTGGGTCACGAGGCATGGGGCGGAGCCGTTGCCGTCGGAGGATGAGAAC TGCACGCTGGTCGAAGTGACTGAAGAGGAGGTCGAGAACTCAGTCAAACACATTCCCAGCTTGGCAACC GTGATCCTGGTGAAGACCATGATACGTAAACGCTCCTTTGGGAACCCATTCGAGGGCAGCCGGCGGGAG GAACGCTCACTGTCAGCGCCTGGAAACTTGCTCACCAAAAAACCAACCAGGGAATGTGAGTCCCTGTCT GAGCTCAAGGGGACAAAAAAAAAAAAAGGACTTGACTCCATGACGTCGACCGTGGCCGCTGGCTGGCTG GACAGGCGGGTGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001270486
Insert Size	1671 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001270486.1
RefSeq Size	4523 bp
RefSeq ORF	1671 bp
Locus ID	10645
UniProt ID	Q96RR4
Protein Families	Druggable Genome, Protein Kinase, Transcription Factors
Protein Pathways	Adipocytokine signaling pathway
MW	61.4 kDa
Gene Summary	The product of this gene belongs to the Serine/Threonine protein kinase family, and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. The major isoform of this gene plays a role in the calcium/calmodulin-dependent (CaM) kinase cascade by phosphorylating the downstream kinases CaMK1 and CaMK4. Protein products of this gene also phosphorylate AMP-activated protein kinase (AMPK). This gene has its strongest expression in the brain and influences signalling cascades involved with learning and memory, neuronal differentiation and migration, neurite outgrowth, and synapse formation. Alternative splicing results in multiple transcript variants encoding distinct isoforms. The identified isoforms differ in their ability to undergo autophosphorylation and to phosphorylate downstream kinases. [provided by RefSeq, Jul 2012] Transcript Variant: This variant (9) lacks a segment in the 3' coding region and 3' UTR, which leads to a frameshift, compared to variant 1. The resulting isoform (7) contains a shorter and distinct C-terminus compared to isoform 1.

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