ARPP21 (NM_001267619) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ARPP-21; R3HDM3; RCS; TARPP
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332870 representing NM_001267619. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGTCTGAGCAAGGAGACCTGAATCAGGCAATAGCAGAGGAAGGAGGGACTGAGCAGGAGACGGCCACT CCAGAGAACGGCATTGTTAAATCAGAAAGTCTGGATGAAGAGGAGAAACTGGAACTGCAGAGGCGGCTG GAGGCTCAGAATCAAGAAAGAAGAAAATCCAAGTCAGGAGCAGGAAAAGGTAAACTGACTCGCAGCCTT GCTGTCTGTGAGGAATCTTCTGCCAGACCAGGAGGTGAAAGTCTTCAGGATCAGGAATCAATTCATTTA CAGCTTTCCAGTTTTTCCAGCCTGCAAGAGGAGGATAAATCTAGGAAAGATGACTCTGAAAGAGAAAAA GAAAAGGATAAAAACAAAGATAAAACCTCTGAAAAACCCAAGATCAGAATGTTATCAAAAGATTGCAGC CAAGAATACACGGATTCTACAGGCATAGACTTACACGAGTTTCTGATTAACACATTAAAGAATAATTCC AGGGACAGGATGATACTTTTGAAAATGGAGCAGGAAATTATTGATTTCATTGCTGACAACAATAATCAT TATAAAAAGTTCCCTCAGATGTCATCGTATCAGAGGATGCTTGTCCATCGAGTGGCAGCTTATTTTGGA TTGGATCACAATGTGGATCAAACAGGAAAATCTGTTATCATCAACAAGACCAGCAGCACCAGAATACCA GAGCAAAGGTTTTGTGAACATTTAAAAGATGAAAAAGGTGAAGAATCCCAGAAGCGGTTTATCTTGAAG CGAGATAACTCTAGTATTGATAAAGAAGACAATCAGTCAGTTTGCTCCCAGGAAAGCCTTTTTGTGGAA AACAGTAGGCTCTTGGAAGACAGTAACATATGCAATGAGACCTATAAGAAAAGACAGCTCTTTCGGGGC AACAGAGATGGCTCAGGGAGAACATCTGGGAGTCGACAGAGCAGCTCAGAAAATGAACTCAAGTGGTCT GACCACCAAAGGGCCTGGAGCAGCACAGACTCCGACAGTTCCAACCGCAATCTAAAGCCCGCCATGACC AAGACGGCGAGTTTTGGGGGCATCACGGTGCTGACCAGGGGTGACAGCACTTCCAGTACTAGGAGTACC GGGAAGCTGTCCAAAGCAGGTTCCGAGTCTTCCAGCAGTGCAGGCTCCTCAGGATCGCTGTCCCGCACC CATCCACCTCTCCAGAGCACACCCCTAGTCTCAGGTGTGGCAGCTGGCTCTCCAGGCTGTGTGCCTTAT CCAGAGAATGGAATAGGGGGCCAGGTTGCTCCCAGCAGCACCAGCTACATCCTCCTTCCACTTGAAGCT GCAACAGGCATCCCGCCTGGAAGCATCCTTCTTAATCCACACACAGGCCAGCCCTTTGTGAATCCCGAT GGAACTCCTGCAATATACAACCCACCCACCAGTCAGCAGCCCCTGCGAAGCGCCATGGTGGGGCAGTCC CAACAGCAGCCACCACAGCAGCAGCCCTCCCCGCAGCCCCAACAGCAGGTCCAGCCACCGCAGCCACAG ATGGCAGGCCCTCTGGTCACTCAGTCTGTCCAGGGGCTGCAGGCTTCCTCCCAGTCAGTGCAATATCCA GCAGTCTCTTTTCCTCCCCAGCACCTCCTACCTGTGTCTCCAACGCAGCACTTTCCCATGAGAGATGAT GTGGCAACACAGTTTGGCCAGATGACCCTGAGCCGGCAGTCCTCGGGGGAGACTCCTGAACCCCCATCA GGTCCTGTCTACCCATCCTCCCTTATGCCACAGCCGGCCCAGCAGCCCAGCTATGTAATCGCCTCTACA GGCCAGCAGCTTCCTACAGGAGGATTCTCAGGCTCTGGCCCTCCCATCTCCCAGCAGGTCCTCCAGCCC CCTCCCTCACCACAGGGATTTGTGCAACAGCCTCCGCCTGCACAGATGCCTGTATATTATTACCCATCT GGTCAGTACCCTACCTCAACCACGCAACAGTACCGGCCCATGGCCCCGGTTCAGTACAACGCTCAGAGG AGTCAACAGATGCCACAGGCAGCACAGCAAGCAGGTTACCAGCCAGTCTTGTCTGGTCAACAGGGATTC CAAGGCCTAATAGGAGTGCAGCAGCCACCTCAGAGTCAGAACGTGATAAATAACCAACAAGGAACTCCG GTGCAAAGCGTGATGGTTTCCTACCCAACAATGTCTTCTTATCAGGTGCCAATGACCCAGGGTTCTCAA GGACTGCCCCAGCAGTCATACCAACAGCCAATCATGCTACCTAACCAGGCAGGTCAAGGGTCACTCCCA GCCACTGGAATGCCTGTTTACTGTAATGTCACACCGCCCACCCCTCAGAACAACCTTAGGCTGATTGGC CCACACTGCCCCTCCAGCACTGTCCCAGTGATGTCAGCTAGCTGCAGAACAAACTGTGCAAGTATGAGC AATGCTGGTTGGCAGGTCAAATTCTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001267619
Insert Size	2442 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001267619.1
RefSeq Size	3209 bp
RefSeq ORF	2442 bp
Locus ID	10777
UniProt ID	Q9UBL0
MW	88.6 kDa
Gene Summary	This gene encodes a cAMP-regulated phosphoprotein. The encoded protein is enriched in the caudate nucleus and cerebellar cortex. A similar protein in mouse may be involved in regulating the effects of dopamine in the basal ganglia. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jun 2012] Transcript Variant: This variant (8) has a shorter 5' UTR and lacks two in-frame exons in the central coding region, compared to variant 1. The resulting isoform (4) is shorter, compared to isoform 1.

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