FBXW10 (NM_001267586) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	Fbw10; HREP; SM2SH2; SM25H2
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332862 representing NM_001267586. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGAAAACCTGGAATCAAGGCTCAAGAATGCCCCCTATTTTCGTTGTGAGAAGGGAACCGATTCCATC CCTCTATGCCGGAAGTGTGAGACGTGTGTCTTAGCCTGGAAGATCTTCTCTACCAAAGAGTGGTTCTGC AGGATCAATGACATATCACAGAGGAGGTTTCTAGTTGGCATTCTGAAGCAGTTAAATAGCTTATATTTG TTACACTATTTCCAAAATATCCTTCAGACCACACAGGGAAAGGATTTCATCTATAACAGGTCCCGGATC AACCTCAGCAAGAAAGAGGGGAAAGTTGTGAAGTCCTCCTTGAACCAAATGTTGGATAAAACAGTAGAA CAGAAGATGAAAGAGATCTTGTACTGGTTTGCGAACAGCACCCAGTGGACCAAGGCGAATTATACTCTC TTACTGCTGCAGATGTGCAACCCCAAATTACTGCTCACTGCTGCCAATGTGATCAGAGTCCTGTTTCTG AGAGAGGAGAACAATATCTCAGGGCTCAATCAAGACATCACAGATGTGTGTTTTTCCCCTGAGAAAGAC CACAGCTCCAAGTCTGCGACCTCACAAGTCTATTGGACAGCCAAAACTCAGCACACATCCCTTCCTTTG TCCAAAGCCCCAGAAAATGAACACTTGCTTGGGGCAGCATCTAACCCTGAGGAACCATGGAGGAATTCA CTCCGGTGTATATCCGAAATGAATAGGCTGTTTTCTGGAAAAGGAGACATAACCAAGCCAGGGTACGAT CCCTGCAATCTATTGGTTGACCTGGATGACATCAGAGACCTGTCTTCTGGGTTCAGCAAATACCGAGAC TTCATCCGTTACCTGCCCATCCACCTCTCCAAGTACATTCTAAGAATGCTGGATAGACACACCCTGAAC AAGTGCGCCTCTGTGAGCCAGCACTGGGCCGCCATGGCTCAACAGGTCAAGATGGACTTGTCAGCGCAC GGCTTCATTCAGAACCAGATTACCTTCTTGCAGGGGTCCTACACAAGAGGAATTGATCCTAATTATGCC AATAAGGTTTCTATCCCAGTTCCTAAAATGGTAGATGACGGGAAGAGCATGCGTGTGAAACATCCGAAG TGGAAGCTGAGAACGAAGAATGAGTACAACCTGTGGACTGCATACCAGAACGAGGAAACGCAGCAGGTC CTGATAGAGGAGAGAAATGTTTTCTGTGGGACCTACAATGTTCGCATTCTCTCTGACACGTGGGATCAA AACCGAGTCATCCACTATTCCGGGGGAGATCTGATAGCTGTGTCATCTAATCGAAAGATCCATCTTCTG GACATCATACAAGTGAAAGCGATACCCGTTGAATTCCGAGGCCATGCTGGGAGTGTCCGGGCCCTCTTC CTGTGTGAGGAGGAAAACTTTCTCCTAAGCGGGAGCTATGACCTAAGTATCAGATACTGGGATCTGAAA AGTGGGGTTTGCACACGAATCTTCGGTGGTCACCAGGGGACTATCACTTGCATGGACTTGTGTAAGAAC AGGCTCGTATCTGGAGGAAGAGATTGCCAGGTAAAAGTATGGGATGTAGACACAGGGAAGTGCCTGAAG ACGTTTAGACACAAAGACCCCATCTTGGCCACCAGGATCAATGATACCTACATTGTGAGCAGCTGTGAG CGAGGGCTGGTGAAAGTGTGGCACATTGCCATGGCCCAGTTGGTAAAGACTCTCAGTGGCCATGAGGGA GCCGTGAAATGCCTGTTCTTTGACCAGTGGCATCTCCTCTCAGGAAGTACTGATGGCCTGGTCATGGCC TGGAGCATGGTGGGGAAGTACGAGCGCTGCCTGATGGCCTTCAAGCATCCCAAGATGGTGGTCAACACA GAGAGCAATGTTCTCATGTTCCAGTTTGAGCACATAAAGTGGCAGTATGCCGTGGAAAAAACGAAACAA AAGAAGAATAAGGAGAAAGAGGAGGAAAAAGAAGAAAATAGTCTCATGGAAATTCTCTCTAAGTGTAAT ATTCAGGTTCACAGCCCAAGAGAGTCTGTATCCAGTAAACAAACTGTGATCCAAGAGCTCCTACCAGGC AAACCTCCCAAGTCCCGAGTACTCCTGAAGCCGGCCAAGTTCTCTTCAGCAGTGTTAATAGAGGAACTT CAAAGTCAAGGAAAGTCAAAATCACCCCGAAGAGATGCAGATGATGTGGAGAAAGCACAAAAACAAGGA CAATTGGAAACTCCTGGAAAACTGCCCAGTCACCCAAAGAAAAAGTCTTGGAAAATCCCTATGTCACCT GACCAATTCCTCCTGACTGTTAGCGCCCTGCAGCACGCCCATAATTCCGGGGAATTTGCCTATCCCTGT AGGCCCCAAACAGAAATTACTGATGTCTGGGGACCTTCAATTTCATACCCAAGGAAGGTCTTGAATTTC AAAGGAAAATCAATCCAACGTGCAGTTGATCGGTTGAGATTGAGCAATCCTCCTATAGATGTGAAACGA ACCAGTATTCCCCTTGAAATCCAGAAACTGCAGCCCAACTTGAAGATCTCTTTGCACAGTCCTAGAGTC CAGTCCACCATACCCCAGCCCATGATTATCCGCTCCAGGTTCTCTGGCAGCTTAAAGGGTGGAGACCAA GTGACCAGTTCAATTGAAAGGGCTGTGTGCAGTACGGGTCCCCTGACCAGTATGCAGGTCATTAAACCA AACCGCATGCTAGCTCCACAAGTGGGCACAGCCACCCTGTCTCTTAAGAAAGAACGGCCTCGCATCTAT ACAGCCCTTGATCCTTTTAGAGTGAACACTGAGTTCGTGCTGTTGACCGTGAAGGAGGAGAAGGAGCAC CAGGAAGCCAAGATGAAGGAATATCAGGCCAGGGAGTCCACTGGAGTGGTTGATCCAGGAAAAGTCAGC AAAGCTGCATGGATCAGGAAGATCAAAGGCCTGCCTATTGATAATTTCACGAAGCAAGGGAAAACAGCG GCCCCTGAACTTGGACAAAATGTATTTATCTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001267586
Insert Size	3000 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001267586.1
RefSeq Size	3293 bp
RefSeq ORF	3000 bp
Locus ID	10517
UniProt ID	Q5XX13
MW	114 kDa
Gene Summary	Members of the F-box protein family, such as FBXW10, are characterized by an approximately 40-amino acid F-box motif. SCF complexes, formed by SKP1 (MIM 601434), cullin (see CUL1; MIM 603134), and F-box proteins, act as protein-ubiquitin ligases. F-box proteins interact with SKP1 through the F box, and they interact with ubiquitination targets through other protein interaction domains (Jin et al., 2004 [PubMed 15520277]).[supplied by OMIM, Mar 2008] Transcript Variant: This variant (2) lacks an alternate in-frame exon in the central coding region, compared to variant 1, resulting in an isoform (2) that is shorter than isoform 1.

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