LAT2 (SLC7A8) (NM_001267036) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	LAT2; LPI-PC1
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332831 representing NM_001267036. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGGGCAGTATGGGCAGGAGCTTAGCTGGAAGTGTTTGGTAAAAGCTGTGTGCCTCCAGGAGCACTCA CAGCCCTCACAGCTTCTCTGCACCCTCCTCCTCTGCTGGTGTGTCCTTGGAAGAGAGAGGCCCTTCCGG AAGGCTCAGAGCACCTCCTCTCCTTTGGAGGGGGTTCCGAGATTTCTAAAACGTTTGCTCCTCACATGG GTCAACTGTTCCAGTGTGCGGTGGGCCACCCGGGTTCAAGACATCTTCACAGCTGGGAAGCTCCTGGCC TTGGCCCTGATTATCATCATGGGGATTGTACAGATATGCAAAGGAGAGTACTTCTGGCTGGAGCCAAAG AATGCATTTGAGAATTTCCAGGAACCTGACATCGGCCTCGTCGCACTGGCTTTCCTTCAGGGCTCCTTT GCCTATGGAGGCTGGAACTTTCTGAATTACGTGACTGAGGAGCTTGTTGATCCCTACAAGAACCTTCCC AGAGCCATCTTCATCTCCATCCCACTGGTCACATTTGTGTATGTCTTTGCCAATGTCGCTTATGTCACT GCAATGTCCCCCCAGGAGCTGCTGGCATCCAACGCCGTCGCTGTGACTTTTGGAGAGAAGCTCCTAGGA GTCATGGCCTGGATCATGCCCATTTCTGTTGCCCTGTCCACATTTGGAGGAGTTAATGGGTCTCTCTTC ACCTCCTCTCGGCTGTTCTTCGCTGGAGCCCGAGAGGGCCACCTTCCCAGTGTGTTGGCCATGATCCAC GTGAAGCGCTGCACCCCAATCCCAGCCCTGCTCTTCACATGCATCTCCACCCTGCTGATGCTGGTCACC AGCGACATGTACACACTCATCAACTATGTGGGCTTCATCAACTACCTCTTCTATGGGGTCACGGTTGCT GGACAGATAGTCCTTCGCTGGAAGAAGCCTGATATCCCCCGCCCCATCAAGATCAACCTGCTGTTCCCC ATCATCTACTTGCTGTTCTGGGCCTTCCTGCTGGTCTTCAGCCTGTGGTCAGAGCCGGTGGTGTGTGGC ATTGGCCTGGCCATCATGCTGACAGGAGTGCCTGTCTATTTCCTGGGTGTTTACTGGCAACACAAGCCC AAGTGTTTCAGTGACTTCATTGAGCTGCTAACCCTGGTGAGCCAGAAGATGTGTGTGGTCGTGTACCCC GAGGTGGAGCGGGGCTCAGGGACAGAGGAGGCTAATGAGGACATGGAGGAGCAGCAGCAGCCCATGTAC CAACCCACTCCCACGAAGGACAAGGACGTGGCGGGGCAGCCCCAGCCCTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001267036
Insert Size	1293 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001267036.1
RefSeq Size	3296 bp
RefSeq ORF	1293 bp
Locus ID	23428
UniProt ID	Q9UHI5
Protein Families	Druggable Genome, Transmembrane
MW	48.3 kDa
Gene Summary	Sodium-independent, high-affinity transport of small and large neutral amino acids such as alanine, serine, threonine, cysteine, phenylalanine, tyrosine, leucine, arginine and tryptophan, when associated with SLC3A2/4F2hc. Acts as an amino acid exchanger. Has higher affinity for L-phenylalanine than LAT1 but lower affinity for glutamine and serine. L-alanine is transported at physiological concentrations. Plays a role in basolateral (re)absorption of neutral amino acids. Involved in the uptake of methylmercury (MeHg) when administered as the L-cysteine or D,L-homocysteine complexes, and hence plays a role in metal ion homeostasis and toxicity. Involved in the cellular activity of small molecular weight nitrosothiols, via the stereoselective transport of L-nitrosocysteine (L-CNSO) across the transmembrane. Plays an essential role in the reabsorption of neutral amino acids from the epithelial cells to the bloodstream in the kidney.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) differs in the 5' UTR, lacks a portion of the 5' coding region and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (c) is shorter and has a distinct N-terminus, compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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