CNOT1 (NM_001265612) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	AD-005; CDC39; HPE12; NOT1; NOT1H
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332826 representing NM_001265612. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGAATCTTGACTCGCTCTCGCTGGCCTTGTCTCAAATCAGCTACCTGGTGGACAATTTAACCAAGAAA AATTACCGAGCCAGCCAGCAGGAAATACAGCATATTGTGAATCGGCACGGTCCTGAGGCAGACAGGCAT TTATTACGCTGCCTATTTTCGCATGTGGATTTCAGTGGCGATGGTAAAAGCAGTGGCAAAGATTTCCAT CAGACTCAGTTTCTGATTCAGGAGTGTGCGTTGCTGATTACAAAGCCAAATTTTATCTCGACGCTGTCC TATGCCATTGATAATCCATTGCACTATCAGAAGAGTTTAAAGCCTGCACCCCACTTATTTGCCCAGCTG AGTAAAGTGCTCAAATTAAGCAAAGTACAAGAGGTAATTTTTGGCCTTGCCCTGTTGAATTCTTCCAGC TCAGATCTTAGAGGTTTCGCTGCCCAGTTTATCAAACAGAAGCTTCCAGATCTTCTGCGTTCTTACATT GACGCAGACGTCAGTGGAAATCAAGAAGGTGGCTTCCAAGATATAGCAATAGAGGTCCTACACCTCCTC CTCTCCCATCTCCTCTTTGGGCAGAAGGGAGCCTTTGGAGTTGGACAAGAACAGATAGACGCTTTTCTT AAGACGCTGCGCAGAGATTTTCCCCAAGAACGCTGTCCCGTGGTGCTCGCACCACTTTTATACCCTGAA AAACGGGACATTCTAATGGACAGGATCCTGCCTGATTCCGGAGGGGTAGCTAAAACCATGATGGAGAGC TCTTTGGCTGATTTCATGCAAGAAGTAGGCTATGGCTTTTGTGCAAGTATTGAAGAATGTCGCAATATA ATCGTGCAGTTTGGTGTTCGGGAGGTCACAGCTGCCCAGGTTGCAAGGGTTTTGGGAATGATGGCTCGA ACTCATTCAGGATTAACAGATGGCATTCCATTACAGAGTATTTCTGCTCCGGGCAGTGGGATCTGGAGT GATGGGAAAGATAAAAGTGATGGAGCACAGGCACACACATGGAATGTAGAAGTCTTGATTGACGTTCTT AAAGAACTGAATCCAAGTTTGAATTTCAAGGAAGTAACTTATGAACTGGACCATCCTGGATTTCAAATT CGTGACAGTAAAGGACTTCATAATGTGGTTTATGGCATTCAGAGGGGTTTGGGTATGGAAGTGTTCCCA GTAGACCTCATATATAGACCTTGGAAACATGCTGAAGGCCAGCTCTCCTTCATTCAACATTCCCTTATA AATCCAGAGATCTTCTGTTTTGCTGACTATCCCTGTCATACTGTTGCCACTGATATTCTGAAAGCACCA CCAGAGGATGACAATCGAGAAATTGCCACATGGAAGAGCTTGGATTTGATTGAATCTCTGCTGAGGCTT GCAGAGGTTGGGCAGTATGAGCAAGTCAAACAGCTCTTCAGCTTCCCTATCAAACACTGTCCAGACATG CTGGTATTGGCCTTACTACAAATTAACACCTCTTGGCATACCTTGCGCCATGAACTTATCTCCACTCTG ATGCCAATTTTCCTTGGAAACCATCCTAACTCAGCTATTATTTTGCACTATGCATGGCATGGGCAGGGA CAGTCTCCCTCAATTCGCCAACTTATCATGCATGCAATGGCAGAATGGTACATGAGAGGGGAGCAGTAT GATCAGGCCAAATTGTCTCGAATACTTGATGTGGCCCAGGACTTGAAGGCCTTGTCAATGCTGCTAAAT GGTACTCCATTTGCCTTTGTTATTGACCTTGCTGCACTTGCTTCACGTCGTGAATACCTCAAACTTGAT AAGTGGCTCACAGATAAAATTCGAGAGCATGGGGAGCCTTTTATCCAGGCGTGTATGACTTTTTTAAAG AGACGGTGTCCTTCTATTTTGGGCGGACTTGCCCCAGAAAAAGACCAGCCCAAAAGTGCTCAACTTCCT CCAGAAACTTTGGCGACAATGTTGGCCTGTCTGCAAGCTTGTGCAGGGAGTGTTTCTCAGGAGCTATCA GAAACTATCCTCACCATGGTAGCCAATTGCAGTAATGTTATGAATAAGGCCAGACAACCACCACCTGGA GTTATGCCAAAAGGACGTCCTCCTAGTGCTAGCAGCTTAGATGCCATTTCTCCTGTTCAGATTGACCCT CTTGCTGGAATGACATCTCTTAGTATAGGTGGTTCAGCTGCCCCTCACACCCAGAGTATGCAGGGTTTT CCTCCAAATTTGGGTTCTGCATTCAGTACCCCTCAGTCACCAGCAAAAGCATTTCCACCCCTTTCAACC CCCAATCAGACCACTGCATTCAGTGGTATTGGAGGACTTTCATCACAGCTTCCAGTAGGTGGTCTTGGC ACAGGCAGCCTGACTGGTATAGGAACTGGTGCTCTTGGACTCCCTGCAGTGAATAACGACCCTTTTGTA CAGAGGAAACTGGGCACCTCTGGACTGAATCAGCCTACATTCCAGCAGACGGACTTGTCTCAGGTGTGG CCAGAGGCAAACCAGCACTTTAGTAAAGAGATAGATGATGAAGCAAACAGCTATTTCCAGCGAATATAT AATCATCCACCACATCCAACCATGTCTGTTGATGAGGTATTAGAAATGCTGCAGAGATTTAAAGACTCT ACTATAAAGAGGGAACGAGAAGTATTTAACTGTATGCTAAGGAACTTGTTTGAAGAATATCGTTTTTTT CCCCAGTATCCTGATAAAGAGTTACATATAACAGCCTGCCTATTTGGTGGTATAATTGAGAAAGGACTG GTCACTTACATGGCACTAGGTCTGGCTCTACGATATGTTCTTGAAGCCTTACGCAAGCCTTTTGGATCC AAAATGTATTATTTCGGGATTGCTGCACTAGATAGATTTAAAAACAGATTGAAGGACTATCCCCAGTAT TGTCAGCATTTGGCTTCTATCAGTCACTTTATGCAATTTCCACATCATTTACAGGAGTATATTGAGTAT GGACAGCAGTCTAGAGATCCTCCTGTGAAAATGCAAGGCTCTATCACAACCCCTGGAAGTATTGCACTG GCTCAGGCCCAGGCTCAGGCCCAGGTTCCAGCAAAAGCTCCTCTTGCTGGTCAAGTTAGCACTATGGTA ACCACCTCAACAACTACCACTGTTGCTAAAACGGTTACGGTCACCAGGCCAACTGGAGTCAGCTTTAAG AAAGATGTGCCACCTTCTATTAATACTACAAATATAGATACGTTGCTTGTGGCCACAGATCAAACTGAG AGAATTGTGGAGCCCCCAGAAAATATCCAGGAGAAAATTGCTTTTATTTTCAATAATCTCTCACAGTCA AATATGACACAAAAGGTTGAAGAGCTAAAGGAAACGGTGAAAGAAGAATTTATGCCTTGGGTTTCACAG TATCTGGTTATGAAGAGAGTCAGTATTGAGCCAAACTTTCATAGCCTGTATTCAAACTTCCTTGACACG CTGAAGAATCCTGAATTTAACAAGATGGTTCTGAATGAGACCTACAGAAACATTAAAGTGCTCCTGACC TCTGATAAAGCTGCAGCCAATTTCTCAGATCGTTCTTTGCTGAAGAACTTGGGACATTGGCTAGGAATG ATCACATTAGCTAAAAACAAACCCATCTTACACACTGACTTGGATGTGAAATCATTGCTGCTAGAGGCT TATGTTAAAGGACAACAAGAATTGCTCTATGTAGTGCCCTTTGTTGCCAAAGTCTTAGAATCTAGCATT CGTAGTGTGGTTTTTAGGCCACCAAACCCTTGGACAATGGCAATTATGAATGTATTAGCTGAGCTACAT CAGGAGCATGACTTAAAGTTAAACTTGAAGTTTGAAATCGAGGTTCTCTGCAAGAACCTTGCATTAGAC ATCAATGAGCTAAAACCTGGAAACCTCCTAAAGGATAAAGATCGCCTGAAGAATTTAGATGAGCAACTC TCTGCTCCAAAGAAAGATGTCAAGCAGCCAGAAGAACTCCCTCCCATCACAACCACAACAACTTCTACT ACACCAGCTACCAACACCACTTGTACAGCCACGGTTCCACCACAGCCACAGTACAGCTACCACGACATC AATGTCTATTCCCTTGCGGGCTTGGCACCACACATTACTCTGAATCCAACAATTCCCTTGTTTCAGGCC CATCCACAGTTGAAGCAGTGTGTGCGTCAGGCAATTGAACGGGCTGTCCAGGAGCTGGTCCATCCTGTG GTGGATCGATCAATTAAGATTGCCATGACTACTTGTGAGCAAATAGTCAGGAAGGATTTTGCCCTGGAT TCGGAGGAATCTCGAATGCGAATAGCAGCTCATCACATGATGCGTAACTTGACAGCTGGAATGGCTATG ATTACATGCAGGGAACCTTTGCTCATGAGCATATCTACCAACTTAAAAAACAGTTTTGCCTCAGCCCTT CGTACTGCTTCCCCACAACAAAGAGAAATGATGGATCAGGCAGCTGCTCAATTAGCTCAGGACAATTGT GAGTTGGCTTGCTGTTTTATTCAGAAGACTGCAGTAGAAAAAGCAGGCCCTGAGATGGACAAGAGATTA GCAACTGAATTTGAGCTGAGAAAACATGCTAGGCAAGAAGGACGCAGATACTGTGATCCTGTTGTTTTA ACATATCAAGCTGAACGGATGCCAGAGCAAATCAGGCTGAAAGTTGGTGGTGTGGACCCAAAGCAGTTG GCTGTTTACGAAGAGTTTGCACGCAATGTTCCTGGCTTCTTACCTACAAATGACTTAAGTCAGCCCACG GGATTTTTAGCCCAGCCCATGAAGCAAGCTTGGGCAACAGATGATGTAGCTCAGATTTATGATAAGTGT ATTACAGAACTGGAGCAACATCTACATGCCATCCCACCAACTTTGGCCATGAACCCTCAAGCTCAGGCT CTTCGAAGTCTCTTGGAGGTTGTAGTTTTATCTCGAAACTCTCGGGATGCCATAGCTGCTCTTGGATTG CTCCAAAAGGCTGTAGAGGGCTTACTAGATGCCACAAGTGGTGCTGATGCTGACCTTCTGCTGCGCTAC AGGGAATGCCACCTCTTGGTCCTAAAAGCTCTGCAGGATGGCCGGGCATATGGGTCTCCATGGTGCAAC AAACAGATCACAAGGTGCCTAATTGAATGTCGAGATGAATATAAATATAATGTGGAGGCTGTGGAGCTG CTAATTCGCAATCATTTGGTTAATATGCAGCAGTATGATCTTCACCTAGCGCAGTCAATGGAGAATGGC TTAAACTACATGGCTGTGGCATTTGCTATGCAGTTAGTAAAAATCCTGCTGGTGGATGAAAGGAGTGTT GCTCATGTTACTGAGGCAGATCTGTTCCACACCATTGAAACCCTCATGAGGATTAATGCTCATTCCAGA GGCAATGCTCCAGAAGGATTGCCCCAGCTGATGGAAGTAGTGCGATCCAACTATGAAGCAATGATTGAT CGTGCTCATGGAGGCCCAAACTTTATGATGCATTCTGGGATCTCTCAAGCCTCAGAGTATGATGACCCT CCAGGCCTGAGGGAGAAGGCAGAGTATCTTCTGAGGGAATGGGTGAATCTCTACCATTCAGCAGCAGCT GGCCGCGACAGTACCAAAGCTTTCTCTGCATTTGTTGGACAGATGCACCAGCAAGGAATACTGAAGACC GATGATCTCATAACAAGGTTCTTTCGTCTGTGTACTGAAATGTGTGTTGAAATCAGTTACCGTGCTCAG GCTGAGCAGCAGCACAATCCTGCTGCCAATCCCACCATGATCCGAGCCAAGTGCTATCACAACCTGGAT GCCTTTGTTCGACTCATTGCACTGCTCGTGAAACACTCAGGGGAGGCCACCAACACTGTCACAAAGATT AATCTGCTGAACAAGGTCCTTGGTATAGTAGTGGGAGTTCTCCTTCAGGATCATGATGTTCGTCAGAGT GAATTTCAGCAACTTCCCTACCATCGAATTTTTATCATGCTTCTCTTGGAACTCAATGCACCTGAGCAT GTGTTGGAAACCATTAATTTCCAGACACTTACAGCTTTCTGCAATACATTCCACATCTTGAGGCCTACC AAAGCTCCTGGCTTTGTATATGCCTGGCTTGAACTGATTTCCCATCGGATATTTATTGCAAGAATGCTG GCACATACGCCACAGCAGAAGGGGTGGCCTATGTATGCACAGCTACTGATTGATTTATTCAAATATTTA GCGCCTTTCCTTAGAAATGTGGAACTCACCAAACCTATGCAAATCCTCTACAAGGGCACTTTAAGAGTG CTGCTGGTTCTTTTGCATGATTTCCCAGAGTTCCTTTGTGATTACCATTATGGGTTCTGTGATGTGATC CCACCTAATTGTATCCAGTTAAGAAATTTGATCCTGAGTGCCTTTCCAAGAAACATGAGGCTCCCCGAC CCATTCACTCCTAATCTAAAGGTGGACATGTTGAGTGAAATTAACATTGCTCCCCGGATTCTCACCAAT TTCACTGGAGTAATGCCACCTCAGTTCAAAAAGGATTTGGATTCCTATCTTAAAACTCGATCACCAGTC ACTTTCCTGTCTGATCTGCGCAGCAACCTACAGGTATCCAATGAACCTGGGAATCGCTACAACCTCCAG CTCATCAATGCACTGGTGCTCTATGTCGGGACTCAGGCCATTGCGCACATCCACAACAAGGGCAGCACA CCTTCAATGAGCACCATCACTCACTCAGCACACATGGATATCTTCCAGAATTTGGCTGTGGACTTGGAC ACTGAGGGTCGCTATCTCTTTTTGAATGCAATTGCAAATCAGCTCCGGTACCCAAATAGCCACACTCAC TACTTCAGTTGCACCATGCTGTACCTTTTTGCAGAGGCCAATACGGAAGCCATCCAAGAACAGATCACA AGAGTTCTCTTGGAACGGTTGATTGTAAATAGGCCACATCCTTGGGGTCTTCTTATTACCTTCATTGAG CTGATTAAAAACCCAGCGTTTAAGTTCTGGAACCATGAATTTGTACACTGTGCCCCAGAAATCGAAAAG TTATTCCAGTCGGTCGCACAGTGCTGCATGGGACAGAAGCAGGCCCAGCAAGTAATGGAAGGGACAGGT GCCAGTTAG
Restriction Sites	SgfI-MluI
ACCN	NM_001265612
Insert Size	7116 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001265612.1
RefSeq Size	8461 bp
RefSeq ORF	7116 bp
Locus ID	23019
UniProt ID	A5YKK6
Protein Pathways	RNA degradation
MW	266.4 kDa
Gene Summary	Scaffolding component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. Additional complex functions may be a consequence of its influence on mRNA expression. Its scaffolding function implies its interaction with the catalytic complex module and diverse RNA-binding proteins mediating the complex recruitment to selected mRNA 3' UTRs. Involved in degradation of AU-rich element (ARE)-containing mRNAs probably via association with ZFP36. Mediates the recruitment of the CCR4-NOT complex to miRNA targets and to the RISC complex via association with TNRC6A, TNRC6B or TNRC6C. Acts as a transcriptional repressor. Represses the ligand-dependent transcriptional activation by nuclear receptors. Involved in the maintenance of embryonic stem (ES) cell identity.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) uses an alternate splice site in the coding region, but maintains the reading frame, compared to variant 1. The encoded isoform (c) is shorter than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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