BCAR3 (NM_001261410) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	AND-34; MIG7; NSP2; SH2D3B
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332763 representing NM_001261410. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGCCTAAGGAATGCAGTGCTTTCCACGCCCTCTCAGCAGCTCTGTGCTGCTTCTATCACCGCAAGTCT TTCATTGGAGTCAAGTTCTCCAAGGAGAGGCACATCATGGACAGGACCCCCGAGAAACTGAAGAAGGAG CTGGAGGAGGAGCTGCTCCTGAGCAGCGAGGACCTGCGCAGCCATGCCTGGTACCACGGCCGCATCCCC CGACAGGTGTCTGAAAACCTTGTGCAGCGAGATGGTGACTTCCTAGTTCGTGACTCTCTGTCCAGCCCT GGGAACTTTGTCCTGACCTGTCAGTGGAAGAACCTCGCTCAGCACTTCAAAATCAACCGGACAGTTCTG CGACTCAGCGAGGCCTACAGCCGCGTGCAGTACCAGTTCGAGATGGAGAGCTTCGACTCCATCCCCGGC CTGGTGCGCTGCTACGTGGGCAACCGCCGGCCCATCTCCCAGCAGAGTGGCGCCATCATCTTCCAGCCC ATCAACAGGACGGTGCCTCTGCGGTGCCTGGAGGAGCATTATGGCACCTCCCCAGGCCAGGCCCGGGAG GGCAGCCTCACCAAGGGAAGGCCGGATGTGGCCAAGAGGCTGAGCCTCACCATGGGTGGCGTCCAGGCC CGAGAGCAGAATTTGCCCAGGGGAAACCTCCTCAGAAACAAAGAAAAGAGTGGTAGCCAGCCCGCCTGC CTGGATCACATGCAGGACAGAAGAGCCTTGTCCCTCAAAGCCCACCAGTCAGAGAGCTACCTGCCGATT GGCTGCAAGCTGCCACCTCAGTCCTCGGGTGTGGACACAAGCCCCTGCCCAAACTCACCTGTGTTCAGG ACGGGAAGCGAGCCTGCCCTGAGCCCAGCAGTGGTTCGGAGGGTCTCCTCAGACGCCAGGGCTGGGGAG GCGCTGAGGGGATCAGACAGTCAACTGTGCCCTAAGCCCCCGCCTAAGCCCTGCAAGGTGCCGTTCCTC AAGGTTCCCTCGTCTCCCTCTGCCTGGCTCAACTCAGAGGCCAACTACTGTGAACTGAACCCAGCGTTT GCCACAGGCTGCGGCAGGGGAGCAAAGCTACCCTCATGTGCCCAGGGAAGCCACACAGAACTGCTCACA GCCAAGCAGAATGAGGCGCCAGGTCCCCGGAACTCTGGCGTCAACTACTTGATCCTTGATGATGATGAC AGGGAAAGACCTTGGGAACCTGCGGCAGCTCAGATGGAGAAGGGGCAGTGGGACAAGGGCGAGTTTGTG ACGCCCCTCCTGGAGACTGTCTCCTCCTTCAGGCCCAACGAGTTTGAGTCAAAGTTCCTTCCCCCTGAG AATAAGCCCCTGGAAACAGCAATGTTGAAACGTGCAAAAGAACTGTTCACCAACAACGACCCCAAGGTC ATCGCCCAGCACGTACTGAGCATGGACTGCAGGGTTGCTAGGATACTTGGAGTCTCTGAAGAGATGAGG AGGAACATGGGGGTGAGCTCAGGCCTGGAACTCATTACCTTGCCTCACGGACACCAGCTGCGCCTGGAC ATAATTGAAAGACACAACACAATGGCCATCGGCATTGCAGTGGACATTCTGGGATGCACGGGCACTTTG GAGGACCGAGCGGCCACTCTGAGTAAGATCATCCAGGTGGCGGTGGAACTGAAGGATTCCATGGGGGAC CTCTATTCCTTCTCAGCTCTCATGAAAGCCCTGGAAATGCCACAGATCACAAGGTTAGAAAAGACGTGG ACTGCTCTGCGGCACCAGTACACCCAAACTGCCATTCTCTATGAGAAACAGCTGAAGCCCTTCAGCAAA CTCCTGCATGAAGGCAGAGAGTCCACATGTGTTCCCCCAAACAATGTATCAGTCCCACTGCTGATGCCG CTTGTGACGTTAATGGAGCGCCAGGCTGTGACTTTTGAAGGAACCGACATGTGGGAAAAAAACGACCAG AGCTGTGAAATCATGCTGAACCATTTGGCAACAGCGCGATTCATGGCCGAGGCTGCAGACAGCTACCGG ATGAATGCTGAGAGGATCCTGGCAGGTTTTCAACCAGATGAAGAAATGAATGAAATCTGCAAGACTGAA TTTCAAATGCGATTGCTATGGGGCAGCAAAGGTGCACAAGTCAATCAGACAGAGAGATATGAGAAATTC AACCAGATTTTAACTGCCCTCTCGCGTAAATTGGAACCTCCTCCTGTAAAGCAGGCAGAGCTTTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001261410
Insert Size	2205 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001261410.1
RefSeq Size	2893 bp
RefSeq ORF	2205 bp
Locus ID	8412
UniProt ID	O75815
Protein Families	Druggable Genome
MW	82.4 kDa
Gene Summary	Breast tumors are initially dependent on estrogens for growth and progression and can be inhibited by anti-estrogens such as tamoxifen. However, breast cancers progress to become anti-estrogen resistant. Breast cancer anti-estrogen resistance gene 3 was identified in the search for genes involved in the development of estrogen resistance. The gene encodes a component of intracellular signal transduction that causes estrogen-independent proliferation in human breast cancer cells. The protein contains a putative src homology 2 (SH2) domain, a hall mark of cellular tyrosine kinase signaling molecules, and is partly homologous to the cell division cycle protein CDC48. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2012] Transcript Variant: This variant (4) differs in the 5' UTR, lacks a portion of the 5' coding region and initiates translation at an alternate start codon compared to variant 1. The resulting protein (isoform 2) is shorter and has a distinct N-terminus compared to isoform 1.

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