PKMYT1 (NM_001258451) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	MYT1; PPP1R126
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332718 representing NM_001258451. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGCCCATGCCCACGGAGGGCACCCCGCCACCTCTGAGTGGCACCCCCATCCCAGTCCCAGCCTACTTC CGCCACGCAGAACCTGGATTCTCCCTCAAGAGGCCCAGGGGGCTCAGCCGGAGCCTCCCACCTCCGCCC CCTGCCAAGGGCAGCATTCCCATCAGCCGCCTCTTCCCTCCTCGGACCCCAGGCTGGCACCAGCTGCAG CCCCGGCGGGTGTCATTCCGGGGCGAGGCCTCAGAGACTCTGCAGAGCCCTGGGTATGACCCAAGCCGG CCAGAGTCCTTCTTCCAGCAGAGCTTCCAGAGGCTCAGCCGCCTGGGCCATGGCTCCTACGGAGAGGTC TTCAAGGTGCGCTCCAAGGAGGACGGCCGGCTCTATGCGGTAAAGCGTTCCATGTCACCATTCCGGGGC CCCAAGGACCGGGCCCGCAAGTTGGCCGAGGTGGGCAGCCACGAGAAGGTGGGGCAGCACCCATGCTGC GTGCGGCTGGAGCAGGCCTGGGAGGAGGGCGGCATCCTGTACCTGCAGACGGAGCTGTGCGGGCCCAGC CTGCAGCAACACTGTGAGGCCTGGGGTGCCAGCCTGCCTGAGGCCCAGGTCTGGGGCTACCTGCGGGAC ACGCTGCTTGCCCTGGCCCATCTGCACAGCCAGGGCCTGGTGCACCTTGATGTCAAGCCTGCCAACATC TTCCTGGGGCCCCGGGGCCGCTGCAAGCTGGGTGACTTCGGACTGCTGGTGGAGCTGGGTACAGCAGGA GCTGGTGAGGTCCAGGAGGGAGACCCCCGCTACATGGCCCCCGAGCTGCTGCAGGGCTCCTATGGGACA GCAGCGGATGTGTTCAGTCTGGGCCTCACCATCCTGGAAGTGGCATGCAACATGGAGCTGCCCCACGGT GGGGAGGGCTGGCAGCAGCTGCGCCAGGGCTACCTGCCCCCTGAGTTCACTGCCGGTCTGTCTTCCGAG CTGCGTTCTGTCCTTGTCATGATGCTGGAGCCAGACCCCAAGCTGCGGGCCACGGCCGAGGCCCTGCTG GCACTGCCTGTGTTGAGGCAGCCGCGGGCCTGGGGTGTGCTGTGGTGCATGGCAGCGGAGGCCCTGAGC CGAGGGTGGGCCCTGTGGCAGGCCCTGCTTGCCCTGCTCTGCTGGCTCTGGCATGGGCTGGCTCACCCT GCCAGCTGGCTACAGCCCCTGGGCCCGCCAGCCACCCCGCCTGGCTCACCACCCTGCAGTTTGCTCCTG GACAGCAGCCTCTCCAGCAACTGGGATGACGACAGCCTAGGGCCTTCACTCTCCCCTGAGGCTGTCCTG GCCCGGACTGTGGGGAGCACCTCCACCCCCCGGAGCAGGTGCACACCCAGGGATGCCCTGGACCTAAGT GACATCAACTCAGAGCCTCCTCGGGGCTCCTTCCCCTCCTTTGAGCCTCGGAACCTCCTCAGCCTGTTT GAGGACACCCTAGACCCAACCTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001258451
Insert Size	1473 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001258451.1
RefSeq Size	2051 bp
RefSeq ORF	1473 bp
Locus ID	9088
UniProt ID	Q99640
Protein Families	Druggable Genome, Protein Kinase
Protein Pathways	Cell cycle, Oocyte meiosis, Progesterone-mediated oocyte maturation
MW	53.5 kDa
Gene Summary	This gene encodes a member of the serine/threonine protein kinase family. The encoded protein is a membrane-associated kinase that negatively regulates the G2/M transition of the cell cycle by phosphorylating and inactivating cyclin-dependent kinase 1. The activity of the encoded protein is regulated by polo-like kinase 1. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012] Transcript Variant: This variant (4) differs in the 5' UTR, lacks a portion of the 5' coding region and initiates translation at a downstream, in-frame start codon, compared to variant 1. The encoded isoform (4) has a shorter N-terminus, compared to isoform 1.

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