TOP1MT (NM_001258447) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332714 representing NM_001258447. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGTTAGATCATGAATACACAACAAAGGAGGTTTTCCGGAAGAACTTCTTCAATGACTGGCGAAAGGAA ATGGCGGTGGAAGAGAGGGAAGTCATCAAGAGCCTGGACAAGTGTGACTTCACGGAGATCCACAGATAC TTTGTGGACAAGGCCGCAGCCCGGAAAGTCCTGAGCAGGGAGGAGAAGCAGAAGCTAAAAGAAGAGGCA GAAAAACTTCAGCAAGAGTTCGGCTACTGTATTTTAGATGGTCACCAAGAAAAAATAGGCAACTTCAAG ATTGAGCCGCCTGGCTTGTTCCGTGGCCGTGGCGACCATCCCAAGATGGGGATGCTGAAGAGAAGGATC ACGCCAGAGGATGTGGTTATCAACTGCAGCAGGGACTCGAAGATCCCCGAGCCGCCGGCGGGGCACCAG TGGAAGGAGGTGCGCTCCGATAACACCGTCACGTGGCTGGCAGCTTGGACCGAGAGCGTTCAGAACTCC ATCAAGTACATCATGCTGAACCCTTGCTCGAAGCTGAAGGGGGAGACAGCTTGGCAGAAGTTTGAAACA GCTCGACGCCTGCGGGGATTTGTGGACGAGATCCGCTCCCAGTACCGGGCTGACTGGAAGTCTCGGGAA ATGAAGACGAGACAGCGGGCGGTGGCCCTGTATTTCATCGATAAGCTGGCACTGAGAGCAGGAAATGAG AAGGAGGACGGTGAGGCGGCCGACACCGTGGGCTGCTGTTCCCTCCGCGTGGAGCACGTCCAGCTGCAC CCGGAGGCCGATGGCTGCCAACACGTGGTGGAATTTGACTTCCTGGGGAAGGACTGCATCCGCTACTAC AACAGAGTGCCGGTGGAGAAGCCGGTGTACAAGAACTTACAGCTCTTTATGGAGAACAAGGACCCCCGG GACGACCTCTTCGACAGGCTGACCACGACCAGCCTGAACAAGCACCTCCAGGAGCTGATGGACGGGCTG ACGGCCAAGGTGTTCCGGACCTACAACGCCTCCATCACTCTGCAGGAGCAGCTGCGGGCCCTGACGCGC GCCGAGGACAGCATAGCAGCTAAGATCTTATCCTACAACCGAGCCAACCGAGTCGTGGCCATTCTCTGC AACCATCAGCGAGCAACCCCCAGTACGTTCGAGAAGTCGATGCAGAATCTCCAGACGAAGATCCAGGCA AAGAAGGAGCAGGTGGCTGAGGCCAGGGCAGAGCTGAGGAGGGCGAGGGCTGAGCACAAAGCCCAAGGG GATGGCAAGTCCAGGAGTGTCCTGGAGAAGAAGAGGCGGCTCCTGGAGAAGCTGCAGGAGCAGCTGGCG CAGCTGAGTGTGCAGGCCACGGACAAGGAGGAGAACAAGCAGGTGGCCCTGGGCACGTCCAAGCTCAAC TACCTGGACCCCAGGATCAGCATTGCCTGGTGCAAGCGGTTCAGGGTGCCAGTGGAGAAGATCTACAGC AAAACACAGCGGGAGAGGTTCGCCTGGGCTCTCGCCATGGCAGGAGAAGACTTTGAATTCTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001258447
Insert Size	1512 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001258447.1
RefSeq Size	1904 bp
RefSeq ORF	1512 bp
Locus ID	116447
UniProt ID	Q969P6
Protein Families	Druggable Genome
MW	58.6 kDa
Gene Summary	This gene encodes a mitochondrial DNA topoisomerase that plays a role in the modification of DNA topology. The encoded protein is a type IB topoisomerase and catalyzes the transient breaking and rejoining of DNA to relieve tension and DNA supercoiling generated in the mitochondrial genome during replication and transcription. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012] Transcript Variant: This variant (3) differs in the 5' UTR, lacks a portion of the 5' coding region and initiates translation at a downstream, in-frame start codon, compared to variant 1. Variants 2 and 3 encode the same isoform (2), which has a shorter N-terminus compared to isoform 1.

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