Netrin G1 ligand (LRRC4C) (NM_001258419) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	NGL-1; NGL1
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332699 representing NM_001258419. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGTTGAACAAGATGACCTTACATCCACAGCAGATAATGATAGGTCCTAGGTTTAACAGGGCCCTATTT GACCCCCTGCTTGTGGTGCTGCTGGCTCTTCAACTTCTTGTGGTGGCTGGTCTGGTGCGGGCTCAGACC TGCCCTTCTGTGTGCTCCTGCAGCAACCAGTTCAGCAAGGTGATTTGTGTTCGGAAAAACCTGCGTGAG GTTCCGGATGGCATCTCCACCAACACACGGCTGCTGAACCTCCATGAGAACCAAATCCAGATCATCAAA GTGAACAGCTTCAAGCACTTGAGACACTTGGAAATCCTACAGTTGAGTAGGAACCATATCAGAACCATT GAAATTGGGGCTTTCAATGGTCTGGCGAACCTCAACACTCTGGAACTCTTTGACAATCGTCTTACTACC ATCCCGAATGGAGCTTTTGTATACTTGTCTAAACTGAAGGAGCTCTGGTTGCGAAACAACCCCATTGAA AGCATCCCTTCTTATGCTTTTAACAGAATTCCTTCTTTGCGCCGACTAGACTTAGGGGAATTGAAAAGA CTTTCATACATCTCAGAAGGTGCCTTTGAAGGTCTGTCCAACTTGAGGTATTTGAACCTTGCCATGTGC AACCTTCGGGAAATCCCTAACCTCACACCGCTCATAAAACTAGATGAGCTGGATCTTTCTGGGAATCAT TTATCTGCCATCAGGCCTGGCTCTTTCCAGGGTTTGATGCACCTTCAAAAACTGTGGATGATACAGTCC CAGATTCAAGTGATTGAACGGAATGCCTTTGACAACCTTCAGTCACTAGTGGAGATCAACCTGGCACAC AATAATCTAACATTACTGCCTCATGACCTCTTCACTCCCTTGCATCATCTAGAGCGGATACATTTACAT CACAACCCTTGGAACTGTAACTGTGACATACTGTGGCTCAGCTGGTGGATAAAAGACATGGCCCCCTCG AACACAGCTTGTTGTGCCCGGTGTAACACTCCTCCCAATCTAAAGGGGAGGTACATTGGAGAGCTCGAC CAGAATTACTTCACATGCTATGCTCCGGTGATTGTGGAGCCCCCTGCAGACCTCAATGTCACTGAAGGC ATGGCAGCTGAGCTGAAATGTCGGGCCTCCACATCCCTGACATCTGTATCTTGGATTACTCCAAATGGA ACAGTCATGACACATGGGGCGTACAAAGTGCGGATAGCTGTGCTCAGTGATGGTACGTTAAATTTCACA AATGTAACTGTGCAAGATACAGGCATGTACACATGTATGGTGAGTAATTCCGTTGGGAATACTACTGCT TCAGCCACCCTGAATGTTACTGCAGCAACCACTACTCCTTTCTCTTACTTTTCAACCGTCACAGTAGAG ACTATGGAACCGTCTCAGGATGAGGCACGGACCACAGATAACAATGTGGGTCCCACTCCAGTGGTCGAC TGGGAGACCACCAATGTGACCACCTCTCTCACACCACAGAGCACAAGGTCGACAGAGAAAACCTTCACC ATCCCAGTGACTGATATAAACAGTGGGATCCCAGGAATTGATGAGGTCATGAAGACTACCAAAATCATC ATTGGGTGTTTTGTGGCCATCACACTCATGGCTGCAGTGATGCTGGTCATTTTCTACAAGATGAGGAAG CAGCACCATCGGCAAAACCATCACGCCCCAACAAGGACTGTTGAAATTATTAATGTGGATGATGAGATT ACGGGAGACACACCCATGGAAAGCCACCTGCCCATGCCTGCTATCGAGCATGAGCACCTAAATCACTAT AACTCATACAAATCTCCCTTCAACCACACAACAACAGTTAACACAATAAATTCAATACACAGTTCAGTG CATGAACCGTTATTGATCCGAATGAACTCTAAAGACAATGTACAAGAGACTCAAATCTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001258419
Insert Size	1923 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001258419.1
RefSeq Size	2795 bp
RefSeq ORF	1923 bp
Locus ID	57689
UniProt ID	Q9HCJ2
Protein Families	Druggable Genome, Transmembrane
Protein Pathways	Axon guidance
MW	71.9 kDa
Gene Summary	NGL1 is a specific binding partner for netrin G1 (NTNG1; MIM 608818), which is a member of the netrin family of axon guidance molecules (Lin et al., 2003 [PubMed 14595443]).[supplied by OMIM, Mar 2008] Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same protein.

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