ARHGAP20 (NM_001258417) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	RARHOGAP
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332697 representing NM_001258417. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGAAAACACTAGCAGAAAGGAGGAGGAGCGCTCCATCTCTTATCCTGGATAAAGCCCTACAAAAACGG CCTACTACCAGGGACAGTCCTTCTGCTAGTGTTGACACATGCACATTTCTGTCATCATTAGTGTGCTCC AATAGGACTCTGCTGATTGATGGCCGGGCAGAACTCAAAAGAGGCCTCCAGAGGCAGGAGCGGCATCTT TTCCTATTCAATGATCTGTTTGTTGTGGCCAAAATCAAATATAACAATAACTTTAAGATAAAAAATAAA ATTAAATTAACTGATATGTGGACAGCAAGCTGTGTGGATGAAGTGGGAGAAGGCAACACCAATGCCATG AAATCCTTTGTTTTGGGCTGGCCCACAGTGAACTTTGTGGCCACTTTCAGTTCTCCAGAACAAAAGGAC AAATGGCTCTCTCTCCTTCAGAGATACATCAATCTAGAGAAAGAAAAGGACTACCCGAAGAGCATTCCC CTCAAAATCTTCGCCAAGGACATTGGGAATTGTGCCTACTCTAAAACTATAACAGTAATGAATTCAGAT ACAGCGAATGAAGTTATCAACATGTCATTACCAATGCTAGGGATAACTGGCTCTGAGAGAGATTACCAG TTGTGGGTCAATTCTGGCAAAGAAGAGGCTCCATACCCACTCATTGGGCATGAATATCCATATGGAATT AAAATGAGCCATCTTCGAGACTCTGCACTCCTGACACCGGGATCAAAGGACTCTACCACCCCTTTCAAC CTCCAGGAGCCCTTCCTTATGGAACAGCTCCCCCGAGAGATGCAGTGCCAGTTCATCCTGAAGCCCAGC CGCCTGGCTGCAGCCCAGCAACTGAGTGATTCAGGTCATAAGACATTTAAAAGGAGAAGATCTATCATA AACTGGGCCTTCTGGCGAGGTTCTAGCACTCACCTGGACAACTTGCCCTCATCGCCAACATCACCTATG CCAGGACAGCTCTTTGGAATTTCTCTGCCAAATATTTGTGAGAATGACAATCTGCCCAAACCTGTCTTG GATATGCTTTTCTTTCTTAATCAAAAAGGACCTCTCACCAAAGGTATCTTCAGGCAATCAGCCAATGTG AAATCCTGCAGAGAACTAAAAGAGAAATTGAATTCTGGAGTCGAAGTACACCTAGACTGTGAATCTATT TTTGTGATAGCATCTGTCTTAAAGGATTTTCTGCGAAATATTCCAGGAAGTATTTTTTCATCAGATCTC TATGATCACTGGGTCTCTGTAATGGATCAAGGAAATGATGAAGAGAAAATAAATACTGTTCAAAGGCTA TTAGACCAGCTTCCGAGAGCCAATGTTGTTCTCCTAAGGTATCTTTTTGGGGTGTTACACAACATTGAG CAACATTCCTCATCCAATCAGATGACTGCATTTAATTTAGCTGTGTGTGTCGCTCCAAGTATTCTTTGG CCTCCTGCTTCCTCCAGCCCAGAACTAGAAAACGAATTTACAAAAAAGGTTTCCCTGCTTATACAATTT CTGATTGAGAATTGCCTTAGGATATTTGGAGAAGAAATCACTTCCCTCTTCAGAGAGGTTTCAGTGAGA TGTGACACTAGAGAGAATGCCTCAGATATTTCTTGCTTTCAACTGAATGACTCCTCCTATGACAGCTTG GAAAATGAGCTAAATGAGGATGTTGATGCACCATGCAGTGACTTGGTAAAGAAACTTGGCCAGGGGAGC AGAAGCATGGACTCTGTCTTAACCCTCAGTGACTATGATCTTGACCAGCCCGAGGTGGAAGGCCTTTTA ACCCTAAGCGACTTTGACTTGGCCCATTCTAAAGATGAAGATGTTCAAATGAAACGGCCTCTTGAATCC AAGCCGGTGAACATTTTAGTGTACACAAAGATCCCACTGCGGGATCATGCCAGGGCCCCATCTGCCATG TGCACACCCAGCTACCTGTCCACAGCTGCAGCAAATGCTGCAAAAAGCCTGAGGCGACACCGGCGTTGC TCAGAGCCCAGCATCGACTATCTGGATTCAAAGCTTTCCTACCTCAGGGAGTTTTATCAGAAAAAGCTA CGCAAGTCCAGCTGTGATGCAATTCTTTCTCAAAAAGATGAAGACTATCTGAAGCAGAATCAACCCCTC CAGGAGGAAGGAAAGACATGTTTTAAACAGAGTTTAGTCACAGGCACTGATGTCAGCAAGAAAAATGCC ACTACTCAAAACACTAAGAAGAAAAGCTTGTCTGGTAGTGAAGGAAATCACGTGAAACTTTTCCCTAAG TCTAAGCCAGTGGCCATTTCTGTGGCATCTTATAGTCCTATGTCCTCACAGGATCATTCCAAGAACCAG CCCTTTGATGTGAATACATCTGGATACTCCCCACCACACACAGCAGATGCCCTCAAGGGTCCAAGGACA CATCGGCGCTGCTCAGAGCCCAACATAGAAGACCAGAACCGCAAGCTGACCTATCTCAGGGGAATTTAT TCAAAGAAACAACATAAAACCAGCTGTGAAGCTGGTCTCTTGCATGGAGAGGAGGATTATCTCAAACGG CATAAGTCTTTGCAAATGGAGGGGCAGAAGCTCATTAATCAGAGTTTAGTCATGGGGATTGAGGTGGGC AAGAGTAGTGCCACAAACCAAAACACTGAGAAGGTTTTACCCCCAAGATTAAACCTTTGCCCAAGGACC AGCTATTCCAGCTTATCCTCCCCAGGCACTTCCCCATCCGGCTCATCAGTAAGCTCCCAAGACAGTGCT TTTTCTCAGATTTCTGAACACTCTGTGTTTACACCCACTGAGACTTCCTCTCCAATAGATTGCACTTTT CAGGCTCAGAGAAAACGGGAAGACCTTTCTCCTGACTTTAGCAATGCCAGCCATGTTTCCGGAATGCCC GGTCCCTCATCAGGGCAGGCTTGCAGCCGCCCAGCCTATACAAAGAAGGACACCATGGAGTGGCATTCA CAAATGCATTCTGTAACTCTTCATCCCAGCACATGGTTGAGAAATGGTGTGGCCAGTTTGAAAAACTGG TCCCTCAAAAAGAAAGCAAAGGCAGCCAGACCAGAGGAAGAGAAAATAGCTTCTCCAAAAGGACCCTTA GAGCCACCCCCACATGCTTCTGGTGTTCCAGAAGCCAACTCACTGCAAGAGGAACAAAAAGACTTGCCC TTAAGGGCAGCTGAAGGACTGTCCCCTGTGCAGTCAGCCCAAAGGTGTAGTTCTTCTCCCTTCCAGGAC TCAGAGAGACACTGTAGCTCTCCATTCAGCCTGGTGGAGAGCAGACTTAAGCTGTGCATGAAGTCACAT GAGGAAATAGAGCCTGGTAGTCAGAGCTCTTCTGGTTCTCTGCCTTGGGAAAGAGCCTCAGCCAGCTCT TGGACTCTAGAGGATGCGACCAGCCCAGACTCAGGGCCTACAGTGGTCTGCGACATTGAGGACAGGTAT TTAACCAAAGACATTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001258417
Insert Size	3468 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001258417.1
RefSeq Size	5899 bp
RefSeq ORF	3468 bp
Locus ID	57569
UniProt ID	Q9P2F6
MW	129 kDa
Gene Summary	The protein encoded by this gene is an activator of RHO-type GTPases, transducing a signal from RAP1 to RHO and impacting neurite outgrowth. [provided by RefSeq, Sep 2016] Transcript Variant: This variant (4) differs in the 5' UTR and initiates translation at a downstream, in-frame start codon, compared to variant 1. Variants 4 and 5 encode the same isoform (4), which has a shorter N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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