DIAPH3 (NM_001258368) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	AN; AUNA1; DIA2; diap3; DRF3; mDia2; NSDAN
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332673 representing NM_001258368. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGAACGGCACCAGCCGCGGCTGCACCACCCGGCCCAAGGCTCAGCCGCTGGGACTCCCTACCCTTCC TCAGCCTCTCTCCGCGGCTGCCGGGAAAGCAAGATGCCGCGCAGGAAGGGCCCCCAACACCCTCCGCCG CCCAGTGGCCCCGAGGAGCCTGGGGAGAAGCGCCCCAAGTTTGAAGATATGAATTTAAATGAAGATAAA AAGGCACCATTGCGGGAAAAGGACTTCAGTATCAAAAAAGAAATGGTGATGCAGTACATTAATACTGCT TCTAAGACAGGAAGTCTTAAGAGAAGCCGACAGATCTCACCTCAGGAATTCATTCATGAGCTGAAAATG GGGTCTGCAGATGAGAGACTTGTCACATGCCTGGAGTCTCTCCGAGTGTCTTTGACCAGCAATCCTGTG AGTTGGGTGGAAAGCTTTGGACATGAAGGGCTTGGATTATTATTAGACATTTTGGAAAAACTGATTAGT GGAAAAATCCAAGAAAAAGTTGTAAAGAAAAATCAACATAAAGTCATACAGTGTCTAAAAGCCCTGATG AATACGCAGTATGGCTTGGAAAGAATTATGAGTGAGGAGAGGAGCCTTTCCTTATTGGCCAAAGCCGTG GATCCCAGACACCCCAATATGATGACAGATGTGGTTAAACTTCTCTCTGCGGTATGCATTGTAGGGGAA GAAAGCATCCTTGAAGAAGTTTTAGAAGCTTTAACTTCAGCTGGTGAAGAAAAAAAAATTGACAGATTT TTTTGTATTGTGGAAGGCCTCCGGCACAATTCAGTTCAACTGCAAGTAGCTTGTATGCAGCTCATCAAT GCCCTGGTTACATCTCCTGATGATTTGGATTTCAGGCTTCACATCAGAAATGAATTTATGCGTTGTGGA TTGAAAGAGATATTGCCAAATTTAAAATGCATTAAGAATGATGGCCTGGATATCCAACTTAAAGTCTTT GATGAGCATAAAGAAGAAGATTTGTTTGAGTTATCCCATCGCCTTGAAGATATTAGAGCTGAACTTGAT GAAGCATATGATGTTTACAACATGGTGTGGAGCACAGTTAAAGAAACTAGAGCAGAGGGATATTTTATT TCTATTCTTCAGCATCTTTTGCTGATTCGAAATGATTATTTTATAAGGCAACAATACTTCAAATTAATT GATGAGTGTGTATCCCAGATTGTATTGCATAGAGATGGAATGGATCCAGACTTCACATATCGAAAAAGA CTAGATTTAGATTTAACCCAGTTTGTAGACATTTGCATAGATCAAGCAAAACTAGAAGAGTTTGAAGAG AAAGCATCAGAACTTTACAAGAAATTTGAAAAAGAGTTTACCGACCACCAAGAAACTCAGGCTGAATTG CAGAAAAAAGAGGCAAAGATTAATGAGCTTCAAGCAGAGCTACAAGCTTTTAAGTCTCAGTTTGGTGCC TTGCCAGCTGATTGTAATATTCCTTTGCCTCCCTCTAAAGAAGGTGGAACTGGCCACTCAGCACTTCCT CCTCCGCCTCCACTGCCTTCTGGTGGAGGGGTGCCGCCTCCACCTCCTCCCCCACCACCTCCTCCACTT CCAGGAATGCGGATGCCATTCAGTGGTCCTGTGCCTCCACCACCTCCCCTGGGATTCCTTGGAGGACAA AATTCTCCTCCTCTACCAATCCTGCCATTTGGGTTGAAACCAAAGAAAGAATTTAAACCTGAAATCAGC ATGAGAAGATTGAATTGGTTAAAGATCAGACCTCATGAAATGACTGAAAACTGTTTCTGGATAAAAGTA AATGAAAATAAGTATGAAAACGTGGATTTGCTTTGTAAACTTGAGAATACATTTTGTTGCCAACAAAAA GAGAGAAGAGAAGAGGAAGATATTGAAGAGAAGAAATCGATTAAGAAAAAAATTAAAGAACTTAAGTTT TTAGATTCTAAAATTGCCCAGAACCTTTCAATCTTCCTGAGCTCTTTTCGGGTGCCATATGAGGAAATC AGAATGATGATATTGGAAGTAGATGAAACACGGTTGGCAGAGTCTATGATTCAGAACTTAATAAAGCAT CTTCCTGATCAAGAGCAATTAAATTCATTGTCTCAGTTCAAGAGTGAATATAGCAACTTATGTGAACCT GAGCAGTTTGTGGTTGTGATGAGCAATGTGAAGAGACTACGGCCACGGCTCAGTGCTATTCTCTTTAAG CTTCAGTTTGAAGAGCAGGTGAACAACATCAAACCTGACATCATGGCTGTCAGTACTGCCTGCGAAGAG ATAAAGAAGAGCAAAAGCTTTAGCAAGTTGCTGGAACTTGTATTGCTAATGGGAAACTACATGAATGCT GGCTCCCGGAATGCTCAAACCTTCGGATTTAACCTTAGCTCTCTCTGTAAACTAAAGGACACAAAATCA GCAGATCAGAAAACAACGCTACTTCATTTCCTGGTAGAAATATGTGAAGAGAAGTACCCTGATATACTG AATTTTGTGGATGATTTGGAACCTTTAGACAAAGCTAGTAAAGTCTCTGTAGAAACGCTGGAAAAGAAT TTGAGGCAGATGGGAAGGCAGCTTCAACAGCTTGAGAAGGAATTGGAAACCTTTCCCCCTCCTGAGGAC TTGCATGACAAGTTTGTGACAAAGATGTCCAGATTTGTTATCAGTGCAAAAGAACAATATGAGACACTT TCGAAGTTACACGAAAACATGGAAAAGTTATACCAGAGTATAATAGGATACTATGCCATTGATGTGAAG AAGGTGTCTGTGGAAGACTTTCTTACTGACCTGAATAACTTCAGAACCACATTCATGCAAGCAATAAAG GAGAATATCAAAAAAAGAGAAGCAGAGGAAAAAGAAAAACGTGTCAGAATAGCTAAAGAATTAGCAGAG CGAGAAAGACTCGAACGCCAACAAAAGAAAAAGCGTTTATTAGAAATGAAGACTGAGGGTGATGAGACA GGAGTGATGGATAATCTGCTGGAGGCCTTGCAGTCCGGGGCTGCCTTCCGCGACAGAAGAAAAAGGACA CCGATGCCAAAAGATGTTCGGCAGAGTCTCAGTCCAATGTCTCAGAGGCCTGTTCTGAAAGTTTGTAAC CATGAAAATCAGAAAGTGCAGTTGACAGAAGGGTCACGTTCACACTACAATATCAATTGCAACTCAACA AGGACTCCAGTCGCCAAGGAGCTTAATTATAATCTAGACACTCATACGTCTACTGGGAGGATCAAGGCA GCTGAGAAGAAGGAAGCGTGTAATGTAGAAAGCAACAGAAAAAAGGAAACGGAACTTCTTGGCTCTTTT TCTAAAAATGAATCAGTTCCCGAAGTTGAAGCCCTGCTGGCAAGATTACGAGCTTTATAA
Restriction Sites	SgfI-MluI
ACCN	NM_001258368
Insert Size	3372 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001258368.1
RefSeq Size	4602 bp
RefSeq ORF	3372 bp
Locus ID	81624
UniProt ID	Q9NSV4
Protein Pathways	Regulation of actin cytoskeleton
MW	129 kDa
Gene Summary	This gene encodes a member of the diaphanous subfamily of the formin family. Members of this family are involved in actin remodeling and regulate cell movement and adhesion. Mutations in this gene are associated with autosomal dominant auditory neuropathy 1. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012] Transcript Variant: This variant (5) lacks two alternate in-frame exons compared to variant 1. The resulting protein (isoform e) is shorter compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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