DIAPH3 (NM_001258366) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	AN; AUNA1; DIA2; diap3; DRF3; mDia2; NSDAN
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332671 representing NM_001258366. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGAACGGCACCAGCCGCGGCTGCACCACCCGGCCCAAGGCTCAGCCGCTGGGACTCCCTACCCTTCC TCAGCCTCTCTCCGCGGCTGCCGGGAAAGCAAGATGCCGCGCAGGAAGGGCCCCCAACACCCTCCGCCG CCCAGTGGCCCCGAGGAGCCTGGGGAGAAGCGCCCCAAGTTTCTGGACAAATTTGCCAGCATAAGAATT CCAGGGAGCAAGAAAGAGAGACCTCCACTTCCCAACCTGAAGACTGCATTTGCAAGCAGTGATTGCTCA GCAGCACCTTTAGAGATGATGGAGAACTTTCCAAAGCCACTGTCAGAGAATGAACTCTTAGAACTTTTT GAAAAAATGATGGAAGATATGAATTTAAATGAAGATAAAAAGGCACCATTGCGGGAAAAGGACTTCAGT ATCAAAAAAGAAATGGTGATGCAGTACATTAATACTGCTTCTAAGACAGGAAGTCTTAAGAGAAGCCGA CAGATCTCACCTCAGGAATTCATTCATGAGCTGAAAATGGGGTCTGCAGATGAGAGACTTGTCACATGC CTGGAGTCTCTCCGAGTGTCTTTGACCAGCAATCCTGTGAGTTGGGTGGAAAGCTTTGGACATGAAGGG CTTGGATTATTATTAGACATTTTGGAAAAACTGATTAGTGGAAAAATCCAAGAAAAAGTTGTAAAGAAA AATCAACATAAAGTCATACAGTGTCTAAAAGCCCTGATGAATACGCAGTATGGCTTGGAAAGAATTATG AGTGAGGAGAGGAGCCTTTCCTTATTGGCCAAAGCCGTGGATCCCAGACACCCCAATATGATGACAGAT GTGGTTAAACTTCTCTCTGCGGTATGCATTGTAGGGGAAGAAAGCATCCTTGAAGAAGTTTTAGAAGCT TTAACTTCAGCTGGTGAAGAAAAAAAAATTGACAGATTTTTTTGTATTGTGGAAGGCCTCCGGCACAAT TCAGTTCAACTGCAAGTAGCTTGTATGCAGCTCATCAATGCCCTGGTTACATCTCCTGATGATTTGGAT TTCAGGCTTCACATCAGAAATGAATTTATGCGTTGTGGATTGAAAGAGATATTGCCAAATTTAAAATGC ATTAAGAATGATGGCCTGGATATCCAACTTAAAGTCTTTGATGAGCATAAAGAAGAAGATTTGTTTGAG TTATCCCATCGCCTTGAAGATATTAGAGCTGAACTTGATGAAGCATATGATGTTTACAACATGGTGTGG AGCACAGTTAAAGAAACTAGAGCAGAGGGATATTTTATTTCTATTCTTCAGCATCTTTTGCTGATTCGA AATGATTATTTTATAAGGCAACAATACTTCAAATTAATTGATGAGTGTGTATCCCAGATTGTATTGCAT AGAGATGGAATGGATCCAGACTTCACATATCGAAAAAGACTAGATTTAGATTTAACCCAGTTTGTAGAC ATTTGCATAGATCAAGCAAAACTAGAAGAGTTTGAAGAGAAAGCATCAGAACTTTACAAGAAATTTGAA AAAGAGTTTACCGACCACCAAGAAACTCAGGCTGAATTGCAGAAAAAAGAGGCAAAGATTAATGAGCTT CAAGCAGAGCTACAAGCTTTTAAGTCTCAGTTTGGTGCCTTGCCAGCTGATTGTAATATTCCTTTGCCT CCCTCTAAAGAAGGTGGAACTGGCCACTCAGCACTTCCTCCTCCGCCTCCACTGCCTTCTGGTGGAGGG GTGCCGCCTCCACCTCCTCCCCCACCACCTCCTCCACTTCCAGGAATGCGGATGCCATTCAGTGGTCCT GTGCCTCCACCACCTCCCCTGGGATTCCTTGGAGGACAAAATTCTCCTCCTCTACCAATCCTGCCATTT GGGTTGAAACCAAAGAAAGAATTTAAACCTGAAATCAGCATGAGAAGATTGAATTGGTTAAAGATCAGA CCTCATGAAATGACTGAAAACTGTTTCTGGATAAAAGTAAATGAAAATAAGTATGAAAACGTGGATTTG CTTTGTAAACTTGAGAATACATTTTGTTGCCAACAAAAAGAGAGAAGAGAAGAGGAAGATATTGAAGAG AAGAAATCGATTAAGAAAAAAATTAAAGAACTTAAGTTTTTAGATTCTAAAATTGCCCAGAACCTTTCA ATCTTCCTGAGCTCTTTTCGGGTGCCATATGAGGAAATCAGAATGATGATATTGGAAGTAGATGAAACA CGGTTGGCAGAGTCTATGATTCAGAACTTAATAAAGCATCTTCCTGATCAAGAGCAATTAAATTCATTG TCTCAGTTCAAGAGTGAATATAGCAACTTATGTGAACCTGAGCAGTTTGTGGTTGTGATGAGCAATGTG AAGAGACTACGGCCACGGCTCAGTGCTATTCTCTTTAAGCTTCAGTTTGAAGAGCAGGTGAACAACATC AAACCTGACATCATGGCTGTCAGTACTGCCTGCGAAGAGATAAAGAAGAGCAAAAGCTTTAGCAAGTTG CTGGAACTTGTATTGCTAATGGGAAACTACATGAATGCTGGCTCCCGGAATGCTCAAACCTTCGGATTT AACCTTAGCTCTCTCTGTAAACTAAAGGACACAAAATCAGCAGATCAGAAAACAACGCTACTTCATTTC CTGGTAGAAATATGTGAAGAGAAGTACCCTGATATACTGAATTTTGTGGATGATTTGGAACCTTTAGAC AAAGCTAGTAAAGTCTCTGTAGAAACGCTGGAAAAGAATTTGAGGCAGATGGGAAGGCAGCTTCAACAG CTTGAGAAGGAATTGGAAACCTTTCCCCCTCCTGAGGACTTGCATGACAAGTTTGTGACAAAGATGTCC AGATTTGTTATCAGTGCAAAAGAACAATATGAGACACTTTCGAAGTTACACGAAAACATGGAAAAGTTA TACCAGAGTATAATAGGATACTATGCCATTGATGTGAAGAAGGTGTCTGTGGAAGACTTTCTTACTGAC CTGAATAACTTCAGAACCACATTCATGCAAGCAATAAAGGAGAATATCAAAAAAAGAGAAGCAGAGGAA AAAGAAAAACGTGTCAGAATAGCTAAAGAATTAGCAGAGCGAGAAAGACTCGAACGCCAACAAAAGAAA AAGCGTTTATTAGAAATGAAGACTGAGGGTGATGAGACAGGAGTGATGGATAATCTGCTGGAGGCCTTG CAGTCCGGGGCTGCCTTCCGCGACAGAAGAAAAAGGACACCGATGCCAAAAGATGTTCGGCAGAGTCTC AGTCCAATGTCTCAGAGGCCTGTTCTGAAAGTTTGTAACCATGAAAATCAGAAAGTGCAGTTGACAGAA GGGTCACGTTCACACTACAATATCAATTGCAACTCAACAAGGACTCCAGTCGCCAAGGAGCTTAATTAT AATCTAGACACTCATACGTCTACTGGGAGGATCAAGGCAGCTGAGAAGAAGGAAGCGTGTAATGTAGAA AGCAACAGAAAAAAGGAAACGGAACTTCTTGGCTCTTTTTCTAAAAATGAATCAGTTCCCGAAGTTGAA GCCCTGCTGGCAAGATTACGAGCTTTATAA
Restriction Sites	SgfI-MluI
ACCN	NM_001258366
Insert Size	3549 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001258366.1
RefSeq Size	4779 bp
RefSeq ORF	3549 bp
Locus ID	81624
UniProt ID	Q9NSV4
Protein Pathways	Regulation of actin cytoskeleton
MW	135.6 kDa
Gene Summary	This gene encodes a member of the diaphanous subfamily of the formin family. Members of this family are involved in actin remodeling and regulate cell movement and adhesion. Mutations in this gene are associated with autosomal dominant auditory neuropathy 1. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012] Transcript Variant: This variant (3) lacks an alternate in-frame exon compared to variant 1. The resulting protein (isoform c) is shorter compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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