HA1 (ARHGAP45) (NM_001258328) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	HA-1; HLA-HA1; HMHA1
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332663 representing NM_001258328. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGAGTCGGGGGCAAAGAGTTCTCAAGGGTCTGCTGGGCTGGGTTTGCACTTGGACCTGGGCCTGGAGA GCCAGACTTGGAGCAAGGGGCTGTGGCCTTCATGTCCTGTGCCCCAGAGATCTGCCCCTCCCACCCGAG GAGCTGCCCAGGAAGGATGGGGCTGACGCGGTGTTCCCCGGACCAAGCCTGGAGCCGCCCGCTGGGTCC TCCGGCGTCAAGGCCACAGGGACCCTCAAGCGGCCCACCAGCCTGAGCCGCCACGCCAGCGCGGCTGGC TTCCCCCTGTCGGGTGCTGCCTCCTGGACACTGGGCCGGAGCCACCGGAGCCCACTGACAGCCGCCAGC CCGGGCGAGCTGCCCACCGAGGGTGCCGGCCCGGACGTCGTCGAGGACATCTCCCATCTGCTGGCGGAC GTGGCCCGCTTCGCTGAGGGCCTTGAGAAACTTAAGGAGTGTGTGTTGCGTGACGACCTCCTTGAGGCC CGCCGCCCGCGGGCCCACGAGTGCCTGGGTGAGGCTCTGCGTGTCATGCATCAGATCATCTCCAAGTAC CCGCTGCTGAACACCGTGGAGACGCTCACCGCAGCCGGCACCCTCATTGCCAAGGTCAAAGCCTTCCAT TATGAGAGCAACAATGATCTGGAGAAACAGGAGTTCGAGAAGGCCCTGGAGACGATTGCTGTGGCCTTC AGTAGCACAGTGTCCGAGTTCCTCATGGGTGAAGTGGACAGCAGCACCCTCCTAGCAGTGCCTCCTGGG GACTCGAGCCAGTCCATGGAAAGCCTGTATGGACCGGGCAGTGAGGGCACGCCTCCCAGCCTGGAAGAC TGTGACGCCGGCTGCCTGCCCGCCGAGGAGGTGGACGTGCTGCTACAGCGCTGTGAGGGGGGCGTGGAT GCCGCACTGCTGTATGCCAAGAACATGGCCAAGTACATGAAGGACCTCATCAGCTACCTGGAGAAGCGG ACGACGCTGGAGATGGAGTTTGCCAAGGGCCTGCAGAAGATCGCTCACAACTGCAGACAGAGCGTCATG CAGGAGCCCCACATGCCGCTCCTGTCCATCTACTCGCTGGCCCTGGAGCAGGACCTGGAGTTCGGCCAC AGCATGGTGCAGGCGGTGGGCACCTTGCAGACCCAGACCTTCATGCAGCCCCTGACCCTGCGGCGGCTT GAACACGAGAAGCGCAGGAAGGAGATCAAGGAGGCCTGGCACCGTGCCCAGAGGAAGCTGCAAGAGGCG GAGTCCAACCTGCGCAAGGCCAAGCAGGGTTACGTGCAGCGCTGCGAGGACCACGACAAGGCTCGCTTC CTCGTGGCCAAGGCGGAGGAGGAGCAGGCTGGCAGCGCGCCGGGAGCAGGCAGCACGGCCACCAAGACC CTGGACAAGCGGCGGCGGCTGGAGGAGGAGGCCAAGAACAAGGCGGAGGAAGCTATGGCCACCTACCGC ACCTGCGTGGCCGACGCGAAGACGCAGAAGCAGGAGCTGGAGGATACCAAGGTGACGGCGCTGCGGCAG ATCCAGGAGGTCATCCGGCAGAGCGACCAAACCATCAAGTCGGCCACGATCTCCTACTACCAGATGATG CATATGCAGACGGCGCCGCTGCCCGTGCACTTCCAGATGCTGTGTGAGAGCAGCAAGCTGTATGACCCA GGCCAGCAGTACGCCTCCCACGTGCGCCAGCTGCAGCGGGACCAGGAGCCCGATGTGCACTACGACTTT GAGCCCCACGTCTCCGCCAACGCCTGGTCCCCCGTCATGCGTGCCCGGAAGAGCAGCTTCAACGTGAGT GATGTGGCGCGGCCGGAGGCTGCCGGGAGCCCCCCAGAAGAAGGCGGGTGCACTGAGGGCACACCTGCC AAGGACCACAGGGCCGGGCGAGGACACCAGGTTCACAAGTCATGGCCGCTCTCGATCTCAGACTCGGAC AGTGGGCTGGACCCCGGCCCTGGCGCAGGGGACTTTAAGAAGTTCGAGCGGACGTCATCCAGTGGTACC ATGTCGTCCACGGAGGAGCTGGTGGACCCAGACGGTGGAGCCGGGGCTTCAGCCTTTGAGCAGGCTGAC CTCAACGGCATGACCCCCGAGCTGCCGGTGGCCGTGCCCAGTGGACCGTTCCGCCACGAGGGGCTGTCC AAGGCGGCCCGTACTCACCGGCTCCGGAAGCTCCGCACGCCCGCCAAGTGCCGCGAGTGCAACAGCTAC GTCTACTTCCAGGGTGCTGAGTGTGAAGAGTGCTGCCTGGCCTGCCACAAGAAATGTCTGGAGACGCTG GCCATACAGTGCGGGCACAAGAAGCTGCAAGGCCGCCTGCAGCTGTTCGGCCAGGACTTCAGCCACGCG GCCCGCAGCGCCCCCGACGGCGTGCCCTTCATCGTCAAGAAGTGCGTCTGCGAGATCGAGCGGCGGGCG CTGCGCACCAAGGGCATCTACCGGGTCAATGGGGTAAAGACACGCGTGGAGAAGCTGTGCCAGGCCTTC GAGAACGGCAAGGAGCTGGTCGAGCTGTCGCAGGCCTCGCCCCACGACATCAGCAACGTCCTCAAGCTC TACCTGCGTCAGCTTCCCGAGCCGCTCATCTCCTTCCGCCTCTACCACGAGCTCGTAGGGCTGGCCAAG GACAGCCTGAAGGCAGAGGCCGAGGCCAAGGCGGCGTCCCGGGGCCGGCAGGACGGCTCGGAGAGCGAG GCAGTGGCGGTGGCCCTGGCAGGTCGGCTGCGGGAGCTCCTGCGGGACCTGCCGCCTGAGAACCGGGCC TCGCTGCAGTACCTGCTGCGTCACCTACGCAGGATCGTGGAGGTGGAGCAGGACAACAAGATGACCCCC GGGAACCTGGGCATCGTGTTCGGGCCCACGCTGCTTCGGCCACGGCCCACCGAGGCCACCGTGTCCCTC TCCTCCCTGGTGGATTATCCCCATCAGGCCCGCGTCATCGAGACTCTCATCGTCCACTACGGCCTGGTC TTCGAGGAGGAGCCGGAGGAGACCCCCGGGGGCCAGGACGAGTCATCCAACCAGCGAGCTGAGGTAGTC GTCCAGGTGCCGTACCTGGAGGCGGGCGAGGCGGTGGTCTACCCGCTGCAGGAGGCGGCGGCGGACGGG TGCAGAGAATCCCGAGTTGTGTCCAACGATTCGGACTCGGACCTAGAGGAGGCCTCCGAGCTGCTGTCC TCATCGGAGGCCAGTGCCCTGGGCCACCTCAGCTTCCTGGAGCAGCAGCAGAGCGAGGCCAGCCTAGAG GTGGCTTCTGGCAGCCACAGCGGCAGTGAGGAGCAGCTGGAGGCCACAGCCCGGGAGGACGGGGACGGG GACGAGGACGGCCCGGCCCAGCAGCTCTCAGGATTCAACACCAACCAGTCCAACAACGTGCTGCAGGCC CCACTGCCCCCCATGAGGCTCCGTGGCGGGCGGATGACACTGGGCTCCTGCAGGGAAAGGCAGCCGGAA TTCGTGTGA
Restriction Sites	SgfI-RsrII
ACCN	NM_001258328
Insert Size	3459 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001258328.2
RefSeq Size	5399 bp
RefSeq ORF	3459 bp
Locus ID	23526
UniProt ID	Q92619
MW	126.5 kDa
Gene Summary	Contains a GTPase activator for the Rho-type GTPases (RhoGAP) domain that would be able to negatively regulate the actin cytoskeleton as well as cell spreading. However, also contains N-terminally a BAR-domin which is able to play an autoinhibitory effect on this RhoGAP activity.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) differs in the 5' UTR and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (2) is longer and has a distinct N-terminus, compared to isoform 1.

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