ECT2 (NM_001258316) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ARHGEF31
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332659 representing NM_001258316. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGCTGAAAATAGTGTATTAACATCCACTACTGGGAGGACTAGCTTGGCAGACTCTTCCATTTTTGAT TCTAAAGTTACTGAGATTTCCAAGGAAAACTTACTTATTGGATCTACTTCATATGTAGAAGAAGAGATG CCTCAGATTGAAACAAGAGTGATATTGGTTCAAGAAGCTGGAAAACAAGAAGAACTTATAAAAGCCTTA AAGGACATTAAAGTGGGCTTTGTAAAGATGGAGTCAGTGGAAGAATTTGAAGGTTTGGATTCTCCGGAA TTTGAAAATGTATTTGTAGTCACGGACTTTCAGGATTCTGTCTTTAATGACCTCTACAAGGCTGATTGT AGAGTTATTGGACCACCAGTTGTATTAAATTGTTCACAAAAAGGAGAGCCTTTGCCATTTTCATGTCGC CCGTTGTATTGTACAAGTATGATGAATCTAGTACTATGCTTTACTGGATTTAGGAAAAAAGAAGAACTA GTCAGGTTGGTGACATTGGTCCATCACATGGGTGGAGTTATTCGAAAAGACTTTAATTCAAAAGTTACA CATTTGGTGGCAAATTGTACACAAGGAGAAAAATTCAGGGTTGCTGTGAGTCTAGGTACTCCAATTATG AAGCCAGAATGGATTTATAAAGCTTGGGAAAGGCGGAATGAACAGGATTTCTATGCAGCAGTTGATGAC TTTAGAAATGAATTTAAAGTTCCTCCATTTCAAGATTGTATTTTAAGTTTCCTGGGATTTTCAGATGAA GAGAAAACCAATATGGAAGAAATGACTGAAATGCAAGGAGGTAAATATTTACCGCTTGGAGATGAAAGA TGCACTCACCTTGTAGTTGAAGAGAATATAGTAAAAGATCTTCCCTTTGAACCTTCAAAGAAACTTTAT GTTGTCAAGCAAGAGTGGTTCTGGGGAAGCATTCAAATGGATGCCCGAGCTGGAGAAACTATGTATTTA TATGAAAAGGCAAATACTCCTGAGCTCAAGAAATCAGTGTCAATGCTTTCTCTAAATACCCCTAACAGC AATCGCAAACGACGTCGTTTAAAAGAAACACTTGCTCAGCTTTCAAGAGAGACAGACGTGTCACCATTT CCACCCCGTAAGCGCCCATCAGCTGAGCATTCCCTTTCCATAGGGTCACTCCTAGATATCTCCAACACA CCAGAGTCTAGCATTAACTATGGAGACACCCCAAAGTCTTGTACTAAGTCTTCTAAAAGCTCCACTCCA GTTCCTTCAAAGCAGTCAGCAAGGTGGCAAGTTGCAAAAGAGCTTTATCAAACTGAAAGTAATTATGTT AATATATTGGCAACAATTATTCAGTTATTTCAAGTACCATTGGAAGAGGAAGGACAACGTGGTGGACCT ATCCTTGCACCAGAGGAGATTAAGACTATTTTTGGTAGCATCCCAGATATCTTTGATGTACACACTAAG ATAAAGGATGATCTTGAAGACCTTATAGTTAATTGGGATGAGAGCAAAAGCATTGGTGACATTTTTCTG AAATATTCAAAAGATTTGGTAAAAACCTACCCTCCCTTTGTAAACTTCTTTGAAATGAGCAAGGAAACA ATTATTAAATGTGAAAAACAGAAACCAAGATTTCATGCTTTTCTCAAGATAAACCAAGCAAAACCAGAA TGTGGACGGCAGAGCCTTGTTGAACTTCTTATCCGACCAGTACAGAGGTTACCCAGTGTTGCATTACTT TTAAATGATCTTAAGAAGCATACAGCTGATGAAAATCCAGACAAAAGCACTTTAGAAAAAGCTATTGGA TCACTGAAGGAAGTAATGACGCATATTAATGAGGATAAGAGAAAAACAGAAGCTCAAAAGCAAATTTTT GATGTTGTTTATGAAGTAGATGGATGCCCAGCTAATCTTTTATCTTCTCACCGAAGCTTAGTACAGCGG GTTGAAACAATTTCTCTAGGTGAGCACCCCTGTGACAGAGGAGAACAAGTAACTCTCTTCCTCTTCAAT GATTGCCTAGAGATAGCAAGAAAACGGCACAAGGTTATTGGCACTTTTAGGAGTCCTCATGGCCAAACC CGACCCCCAGCTTCTCTTAAGCATATTCACCTAATGCCTCTTTCTCAGATTAAGAAGGTATTGGACATA AGAGAGACAGAAGATTGCCATAATGCTTTTGCCTTGCTTGTGAGGCCACCAACAGAGCAGGCAAATGTG CTACTCAGTTTCCAGATGACATCAGATGAACTTCCAAAAGAAAACTGGCTAAAGATGCTGTGTCGACAT GTAGCTAACACCATTTGTAAAGCAGATGCTGAGAATCTTATTTATACTGCTGATCCAGAATCCTTTGAA GTAAATACAAAAGATATGGACAGTACATTGAGTAGAGCATCAAGAGCAATAAAAAAGACTTCAAAAAAG GTTACAAGAGCATTCTCTTTCTCCAAAACTCCAAAAAGAGCTCTTCGAAGGGCTCTTATGACATCCCAC GGCTCAGTGGAGGGAAGAAGTCCTTCCAGCAATGATAAGCATGTAATGAGTCGTCTTTCTAGCACATCA TCATTAGCAGGTATCCCTTCTCCCTCCCTTGTCAGCCTTCCTTCCTTCTTTGAAAGGAGAAGTCATACG TTAAGTAGATCTACAACTCATTTGATATGA
Restriction Sites	SgfI-MluI
ACCN	NM_001258316
Insert Size	2652 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001258316.1
RefSeq Size	4422 bp
RefSeq ORF	2652 bp
Locus ID	1894
UniProt ID	Q9H8V3
Protein Families	Druggable Genome
MW	100.1 kDa
Gene Summary	The protein encoded by this gene is a guanine nucleotide exchange factor and transforming protein that is related to Rho-specific exchange factors and yeast cell cycle regulators. The expression of this gene is elevated with the onset of DNA synthesis and remains elevated during G2 and M phases. In situ hybridization analysis showed that expression is at a high level in cells undergoing mitosis in regenerating liver. Thus, this protein is expressed in a cell cycle-dependent manner during liver regeneration, and is thought to have an important role in the regulation of cytokinesis. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2017] Transcript Variant: This variant (2), as well as variant 3, encodes isoform b.

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