ECT2 (NM_001258315) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ARHGEF31
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332658 representing NM_001258315. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGCTGAAAATAGTGTATTAACATCCACTACTGGGAGGACTAGCTTGGCAGACTCTTCCATTTTTGAT TCTAAAGTTACTGAGATTTCCAAGGAAAACTTACTTATTGGATCTACTTCATATGTAGAAGAAGAGATG CCTCAGATTGAAACAAGAGTGATATTGGTTCAAGAAGCTGGAAAACAAGAAGAACTTATAAAAGCCTTA AAGACTATTAAAATAATGGAAGTCCCTGTTATAAAGATAAAAGAAAGTTGTCCTGGAAAATCGGATGAA AAATTAATAAAAAGTGTTATTAATATGGACATTAAAGTGGGCTTTGTAAAGATGGAGTCAGTGGAAGAA TTTGAAGGTTTGGATTCTCCGGAATTTGAAAATGTATTTGTAGTCACGGACTTTCAGGATTCTGTCTTT AATGACCTCTACAAGGCTGATTGTAGAGTTATTGGACCACCAGTTGTATTAAATTGTTCACAAAAAGGA GAGCCTTTGCCATTTTCATGTCGCCCGTTGTATTGTACAAGTATGATGAATCTAGTACTATGCTTTACT GGATTTAGGAAAAAAGAAGAACTAGTCAGGTTGGTGACATTGGTCCATCACATGGGTGGAGTTATTCGA AAAGACTTTAATTCAAAAGTTACACATTTGGTGGCAAATTGTACACAAGGAGAAAAATTCAGGGTTGCT GTGAGTCTAGGTACTCCAATTATGAAGCCAGAATGGATTTATAAAGCTTGGGAAAGGCGGAATGAACAG GATTTCTATGCAGCAGTTGATGACTTTAGAAATGAATTTAAAGTTCCTCCATTTCAAGATTGTATTTTA AGTTTCCTGGGATTTTCAGATGAAGAGAAAACCAATATGGAAGAAATGACTGAAATGCAAGGAGGTAAA TATTTACCGCTTGGAGATGAAAGATGCACTCACCTTGTAGTTGAAGAGAATATAGTAAAAGATCTTCCC TTTGAACCTTCAAAGAAACTTTATGTTGTCAAGCAAGAGTGGTTCTGGGGAAGCATTCAAATGGATGCC CGAGCTGGAGAAACTATGTATTTATATGAAAAGGCAAATACTCCTGAGCTCAAGAAATCAGTGTCAATG CTTTCTCTAAATACCCCTAACAGCAATCGCAAACGACGTCGTTTAAAAGAAACACTTGCTCAGCTTTCA AGAGAGACAGACGTGTCACCATTTCCACCCCGTAAGCGCCCATCAGCTGAGCATTCCCTTTCCATAGGG TCACTCCTAGATATCTCCAACACACCAGAGTCTAGCATTAACTATGGAGACACCCCAAAGTCTTGTACT AAGTCTTCTAAAAGCTCCACTCCAGTTCCTTCAAAGCAGTCAGCAAGGTGGCAAGTTGCAAAAGAGCTT TATCAAACTGAAAGTAATTATGTTAATATATTGGCAACAATTATTCAGTTATTTCAAGTACCATTGGAA GAGGAAGGACAACGTGGTGGACCTATCCTTGCACCAGAGGAGATTAAGACTATTTTTGGTAGCATCCCA GATATCTTTGATGTACACACTAAGATAAAGGATGATCTTGAAGACCTTATAGTTAATTGGGATGAGAGC AAAAGCATTGGTGACATTTTTCTGAAATATTCAAAAGATTTGGTAAAAACCTACCCTCCCTTTGTAAAC TTCTTTGAAATGAGCAAGGAAACAATTATTAAATGTGAAAAACAGAAACCAAGATTTCATGCTTTTCTC AAGATAAACCAAGCAAAACCAGAATGTGGACGGCAGAGCCTTGTTGAACTTCTTATCCGACCAGTACAG AGGTTACCCAGTGTTGCATTACTTTTAAATGATCTTAAGAAGCATACAGCTGATGAAAATCCAGACAAA AGCACTTTAGAAAAAGCTATTGGATCACTGAAGGAAGTAATGACGCATATTAATGAGGATAAGAGAAAA ACAGAAGCTCAAAAGCAAATTTTTGATGTTGTTTATGAAGTAGATGGATGCCCAGCTAATCTTTTATCT TCTCACCGAAGCTTAGTACAGCGGGTTGAAACAATTTCTCTAGGTGAGCACCCCTGTGACAGAGGAGAA CAAGTAACTCTCTTCCTCTTCAATGATTGCCTAGAGATAGCAAGAAAACGGCACAAGGTTATTGGCACT TTTAGGAGTCCTCATGGCCAAACCCGACCCCCAGCTTCTCTTAAGCATATTCACCTAATGCCTCTTTCT CAGATTAAGAAGGTATTGGACATAAGAGAGACAGAAGATTGCCATAATGCTTTTGCCTTGCTTGTGAGG CCACCAACAGAGCAGGCAAATGTGCTACTCAGTTTCCAGATGACATCAGATGAACTTCCAAAAGAAAAC TGGCTAAAGATGCTGTGTCGACATGTAGCTAACACCATTTGTAAAGCAGATGCTGAGAATCTTATTTAT ACTGCTGATCCAGAATCCTTTGAAGTAAATACAAAAGATATGGACAGTACATTGAGTAGAGCATCAAGA GCAATAAAAAAGACTTCAAAAAAGGTTACAAGAGCATTCTCTTTCTCCAAAACTCCAAAAAGAGCTCTT CGAAGGGCTCTTATGACATCCCACGGCTCAGTGGAGGGAAGAAGTCCTTCCAGCAATGATAAGCATGTA ATGAGTCGTCTTTCTAGCACATCATCATTAGCAGGTATCCCTTCTCCCTCCCTTGTCAGCCTTCCTTCC TTCTTTGAAAGGAGAAGTCATACGTTAAGTAGATCTACAACTCATTTGATATGA
Restriction Sites	SgfI-MluI
ACCN	NM_001258315
Insert Size	2745 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001258315.1
RefSeq Size	4192 bp
RefSeq ORF	2745 bp
Locus ID	1894
UniProt ID	Q9H8V3
Protein Families	Druggable Genome
MW	103.5 kDa
Gene Summary	The protein encoded by this gene is a guanine nucleotide exchange factor and transforming protein that is related to Rho-specific exchange factors and yeast cell cycle regulators. The expression of this gene is elevated with the onset of DNA synthesis and remains elevated during G2 and M phases. In situ hybridization analysis showed that expression is at a high level in cells undergoing mitosis in regenerating liver. Thus, this protein is expressed in a cell cycle-dependent manner during liver regeneration, and is thought to have an important role in the regulation of cytokinesis. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2017] Transcript Variant: This variant (1), as well as variants 8, 9, and 10, encodes the isoform (a).

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