PDE1A (NM_001258314) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CAM-PDE-1A; CAM-PDE 1A; HCAM-1; HCAM1; HSPDE1A
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332657 representing NM_001258314. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGGCAAAAAGATAAACAAACTATTTTGCTTTAATTTTCTAGTTCAGTGTTTTAGGGGTAAATCAAAA CCATCCAAATGTCAGATCAGAAAGAAAGTTAAAAATCATATAGAAAGACTTCTGGATACTGAAGATGAG CTCAGTGACATTCAGACTGACTCAGTCCCATCTGAAGTCCGGGACTGGTTGGCTTCTACCTTTACACGG AAAATGGGGATGACAAAAAAGAAACCTGAGGAAAAACCAAAATTTCGGAGCATTGTGCATGCTGTTCAA GCTGGAATTTTTGTGGAAAGAATGTACCGAAAAACATATCATATGGTTGGTTTGGCATATCCAGCAGCT GTCATCGTAACATTAAAGGATGTTGATAAATGGTCTTTCGATGTATTTGCCCTAAATGAAGCAAGTGGA GAGCATAGTCTGAAGTTTATGATTTATGAACTGTTTACCAGATATGATCTTATCAACCGTTTCAAGATT CCTGTTTCTTGCCTAATCACCTTTGCAGAAGCTTTAGAAGTTGGTTACAGCAAGTACAAAAATCCATAT CACAATTTGATTCATGCAGCTGATGTCACTCAAACTGTGCATTACATAATGCTTCATACAGGTATCATG CACTGGCTCACTGAACTGGAAATTTTAGCAATGGTCTTTGCTGCTGCCATTCATGATTATGAGCATACA GGGACAACAAACAACTTTCACATTCAGACAAGGTCAGATGTTGCCATTTTGTATAATGATCGCTCTGTC CTTGAGAATCACCACGTGAGTGCAGCTTATCGACTTATGCAAGAAGAAGAAATGAATATCTTGATAAAT TTATCCAAAGATGACTGGAGGGATCTTCGGAACCTAGTGATTGAAATGGTTTTATCTACAGACATGTCA GGTCACTTCCAGCAAATTAAAAATATAAGAAACAGTTTGCAGCAGCCTGAAGGGATTGACAGAGCCAAA ACCATGTCCCTGATTCTCCACGCAGCAGACATCAGCCACCCAGCCAAATCCTGGAAGCTGCATTATCGG TGGACCATGGCCCTAATGGAGGAGTTTTTCCTGCAGGGAGATAAAGAAGCTGAATTAGGGCTTCCATTT TCCCCACTTTGTGATCGGAAGTCAACCATGGTGGCCCAGTCACAAATAGGTTTCATCGATTTCATAGTA GAGCCAACATTTTCTCTTCTGACAGACTCAACAGAGAAAATTGTTATTCCTCTTATAGAGGAAGCCTCA AAAGCCGAAACTTCTTCCTATGTGGCAAGCAGCTCAACCACCATTGTGGGGTTACACATTGCTGATGCA CTAAGACGATCAAATACAAAAGGCTCCATGAGTGATGGGTCCTATTCCCCAGACTACTCCCTTGCAGCA GTGGACCTGAAGAGTTTCAAGAACAACCTGGTGGACATCATTCAGCAGAACAAAGAGAGGTGGAAAGAG TTAGCTGCACAAGAAGCAAGAACCAGTTCACAGAAGTGTGAGTTTATTCATCAGTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001258314
Insert Size	1506 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001258314.1
RefSeq Size	2015 bp
RefSeq ORF	1506 bp
Locus ID	5136
UniProt ID	P54750
Protein Families	Druggable Genome
Protein Pathways	Calcium signaling pathway, Progesterone-mediated oocyte maturation, Purine metabolism, Taste transduction
MW	57.5 kDa
Gene Summary	Cyclic nucleotide phosphodiesterases (PDEs) play a role in signal transduction by regulating intracellular cyclic nucleotide concentrations through hydrolysis of cAMP and/or cGMP to their respective nucleoside 5-prime monophosphates. Members of the PDE1 family, such as PDE1A, are Ca(2+)/calmodulin (see CALM1; MIM 114180)-dependent PDEs (CaM-PDEs) that are activated by calmodulin in the presence of Ca(2+) (Michibata et al., 2001 [PubMed 11342109]; Fidock et al., 2002 [PubMed 11747989]).[supplied by OMIM, Oct 2009] Transcript Variant: This variant (5) differs in the 5' and 3' UTRs and uses an alternate start codon, compared to variant 1. The encoded isoform (5) is shorter and has distinct N- and C-termini, compared to isoform 1.

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