TRPV3 (NM_001258205) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	FNEPPK2; OLMS; OLMS1; VRL3
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >SC332590 representing NM_001258205. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGAAAGCCCACCCCAAGGAGATGGTGCCTCTCATGGGCAAGAGAGTTGCTGCCCCCAGTGGGAACCCT GCCGTCCTGCCAGAGAAGAGGCCGGCGGAGATCACCCCCACAAAGAAGAGTGCACACTTCTTCCTGGAG ATAGAAGGGTTTGAACCCAACCCCACAGTTGCCAAGACCTCTCCTCCTGTCTTCTCCAAGCCCATGGAT TCCAACATCCGGCAGTGCATCTCTGGTAACTGTGATGACATGGACTCCCCCCAGTCTCCTCAAGATGAT GTGACAGAGACCCCATCCAATCCCAACAGCCCCAGTGCACAGCTGGCCAAGGAAGAGCAGAGGAGGAAA AAGGGGCGGCTGAAGAAGCGCATCTTTGCAGCCGTGTCTGAGGGCTGCGTGGAGGAGTTGGTAGAGTTG CTGGTGGAGCTGCAGGAGCTTTGCAGGCGGCGCCATGATGAGGATGTGCCTGACTTCCTCATGCACAAG CTGACGGCCTCCGACACGGGGAAGACCTGCCTGATGAAGGCCTTGTTAAACATCAACCCCAACACCAAG GAGATAGTGCGGATCCTGCTTGCCTTTGCTGAAGAGAACGACATCCTGGGCAGGTTCATCAACGCCGAG TACACAGAGGAGGCCTATGAAGGGCAGACGGCGCTGAACATCGCCATCGAGCGGCGGCAGGGGGACATC GCAGCCCTGCTCATCGCCGCCGGCGCCGACGTCAACGCGCACGCCAAGGGGGCCTTCTTCAACCCCAAG TACCAACACGAAGGCTTCTACTTCGGTGAGACGCCCCTGGCCCTGGCAGCATGCACCAACCAGCCCGAG ATTGTGCAGCTGCTGATGGAGCACGAGCAGACGGACATCACCTCGCGGGACTCACGAGGCAACAACATC CTTCACGCCCTGGTGACCGTGGCCGAGGACTTCAAGACGCAGAATGACTTTGTGAAGCGCATGTACGAC ATGATCCTACTGCGGAGTGGCAACTGGGAGCTGGAGACCACTCGCAACAACGATGGCCTCACGCCGCTG CAGCTGGCCGCCAAGATGGGCAAGGCGGAGATCCTGAAGTACATCCTCAGTCGTGAGATCAAGGAGAAG CGGCTCCGGAGCCTGTCCAGGAAGTTCACCGACTGGGCGTACGGGCCCGTGTCATCCTCCCTCTACGAC CTCACCAACGTGGACACCACCACGGACAACTCAGTGCTGGAAATCACTGTCTACAACACCAACATCGAC AACCGGCATGAGATGCTGACCCTGGAGCCGCTGCACACGCTGCTGCATATGAAGTGGAAGAAGTTTGCC AAGCACATGTTCTTTCTGTCCTTCTGCTTTTATTTCTTCTACAACATCACCCTGACCCTCGTCTCGTAC TACCGCCCCCGGGAGGAGGAGGCCATCCCGCACCCCTTGGCCCTGACGCACAAGATGGGGTGGCTGCAG CTCCTAGGGAGGATGTTTGTGCTCATCTGGGCCATGTGCATCTCTGTGAAAGAGGGCATTGCCATCTTC CTGCTGAGACCCTCGGATCTGCAGTCCATCCTCTCGGATGCCTGGTTCCACTTTGTCTTTTTTATCCAA GCTGTGCTTGTGATACTGTCTGTCTTCTTGTACTTGTTTGCCTACAAAGAGTACCTCGCCTGCCTCGTG CTGGCCATGGCCCTGGGCTGGGCGAACATGCTCTACTATACGCGGGGTTTCCAGTCCATGGGTATGTAC AGCGTCATGATCCAGAAGGTCATTTTGCATGATGTTCTGAAGTTCTTGTTTGTATATATCGTGTTTTTG CTTGGATTTGGAGTAGCCTTGGCCTCGCTGATCGAGAAGTGTCCCAAAGACAACAAGGACTGCAGCTCC TACGGCAGCTTCAGCGACGCAGTGCTGGAACTCTTCAAGCTCACCATAGGCCTGGGTGATCTGAACATC CAGCAGAACTCCAAGTATCCCATTCTCTTTCTGTTCCTGCTCATCACCTATGTCATCCTCACCTTTGTT CTCCTCCTCAACATGCTCATTGCTCTGATGGGCGAGACTGTGGAGAACGTCTCCAAGGAGAGCGAACGC ATCTGGCGCCTGCAGAGAGCCAGGACCATCTTGGAGTTTGAGAAAATGTTACCAGAATGGCTGAGGAGC AGATTCCGGATGGGAGAGCTGTGCAAAGTGGCCGAGGATGATTTCCGACTGTGTTTGCGGATCAATGAG GTGAAGTGGACTGAATGGAAGACGCACGTCTCCTTCCTTAACGAAGACCCGGGGCCTGTAAGACGAACA GCAGATTTCAACAAAATCCAAGATTCTTCCAGGAACGACAGCAAAACCACTCTCAATGCATTTGAAGAA GTCGAG
Restriction Sites	NotI-EcoRI
ACCN	NM_001258205
Insert Size	2376 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001258205.1
RefSeq Size	6133 bp
RefSeq ORF	2376 bp
Locus ID	162514
Protein Families	Druggable Genome, Ion Channels: Transient receptor potential, Transmembrane
MW	90.6 kDa
Gene Summary	This gene product belongs to a family of nonselective cation channels that function in a variety of processes, including temperature sensation and vasoregulation. The thermosensitive members of this family are expressed in subsets of sensory neurons that terminate in the skin, and are activated at distinct physiological temperatures. This channel is activated at temperatures between 22 and 40 degrees C. This gene lies in close proximity to another family member gene on chromosome 17, and the two encoded proteins are thought to associate with each other to form heteromeric channels. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer protein (isoform 1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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