ZCWPW1 (NM_001258008) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ZCW1
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332566 representing NM_001258008. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGATGACAACGTTGCAGAATAAAGAAGAATGTGGAAAGGGACCAAAGAGAATCTTTGCCCCACCTGCA CAAAAATCTTACAGCCTGTTACCTTGTAGCCCTAACTCCCCTAAGGAGGAGACCCCGGGGATCAGTTCC CCAGAGACAGAGGCCAGGATAAGCCTGCCAAAGGCCAGTTTAAAGAAGAAAGAGGAAAAAGCAACCATG AAGAATGTTCCAAGCAGGGAACAGGAGAAAAAAAGAAAGGCACAAATCAACAAGCAAGCAGAGAAGAAA GAAAAGGAAAAATCAAGTCTTACCAATGCAGAATTTGAGGAGATTGTCCAGATTGTTCTGCAGAAGTCC CTTCAGGAGTGCTTGGGGATGGGATCTGGCCTTGATTTTGCAGAGACTTCTTGTGCCCAGCCCGTAGTA TCTACCCAATCAGACAAGGAGCCAGGAATTACTGCTTCTGCTACTGATACTGATAATGCTAATGGAGAG GAGGTACCACATACTCAAGAGATTTCAGTGTCTTGGGAAGGTGAAGCTGCCCCTGAGATAAGGACATCT AAGTTAGGCCAGCCAGATCCTGCACCCTCTAAGAAGAAATCCAATAGACTCACCTTAAGCAAAAGAAAG AAGGAAGCTCAAGATGAGAAGGTGGAGAAAACTCAAGGTGGACATGAGCACAGACAGGAAGACCGACTA AAGAAAACAGTTCAGGATCATTCTCAGATCAGGGACCAGCAAAAAGGAGAGATAAGTGGTTTTGGTCAA TGTCTGGTCTGGGTCCAGTGTTCCTTCCCAAACTGTGGGAAATGGAGGCGGCTGTGTGGGAACATTGAC CCCTCAGTTCTCCCAGATAATTGGTCCTGTGATCAGAACACAGATGTGCAGTATAATCGCTGTGATATT CCTGAGGAGACCTGGACAGGGCTTGAGAGTGATGTGGCCTATGCCTCCTACATCCCAGGATCCATCATC TGGGCCAAGCAATACGGTTACCCCTGGTGGCCAGGCATGATAGAATCTGATCCTGACTTAGGGGAATAT TTTCTTTTTACTTCCCATCTTGATTCCCTGCCGTCTAAGTACCATGTGACGTTTTTTGGAGAAACAGTT TCTCGTGCATGGATCCCAGTCAACATGCTAAAGAACTTCCAGGAGCTGTCCCTGGAGCTATCAGTCATG AAAAAGCGCAGAAATGACTGCAGCCAGAAACTGGGGGTGGCCCTGATGATGGCTCAAGAGGCAGAACAG ATCAGCATTCAGGAACGGGTTAACTTGTTTGGTTTCTGGAGCCGATTCAACGGATCTAACAGTAATGGG GAAAGAAAAGACTTACAGCTCTCTGGTTTGAACAGCCCAGGATCCTGCTTAGAGAAAAAGGAGAAAGAG GAAGAGTTGGAAAAGGAGGAAGGAGAGAAAACAGCCCTAAGAAAAAATTTAAAGCTCCCCAGAGCAAGG CCTTGGCAGCCAGCTTTTCAGAGGGAAAAGAAGTTAGAACAGTGCCAAAGAACCTGGGCCTATCAGCGT GTAAGGGGGCCTGCCCCTCATCTGCGAAAGAAGAGCCCAGACACCGGGAACCCCTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001258008
Insert Size	1575 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001258008.1
RefSeq Size	2274 bp
RefSeq ORF	1575 bp
Locus ID	55063
UniProt ID	Q9H0M4
MW	59.3 kDa
Gene Summary	Dual histone methylation reader specific for PRDM9-catalyzed histone marks (H3K4me3 and H3K36me3) (PubMed:32744506, PubMed:20826339). Facilitates the repair of PRDM9-induced meiotic double-strand breaks (DSBs) (By similarity). Essential for male fertility and spermatogenesis (By similarity). Required for meiosis prophase I progression in male but not in female germ cells (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) uses an alternate in-frame splice junction at the 5' end of an exon and lacks two 3' coding exons compared to variant 1, that causes a frameshift. The resulting isoform (2) lacks a 1 aa internal segment and has a shorter and distinct C-terminus compared to isoform 1.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.