AMCase (CHIA) (NM_001258001) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	AMCASE; CHIT2; TSA1902
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332561 representing NM_001258001. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGTTTCTACTCCTGAGAACCGCCAGACTTTCATCACCTCAGTCATCAAATTCCTGCGCCAGTATGAG TTTGACGGGCTGGACTTTGACTGGGAGTACCCTGGCTCTCGTGGGAGCCCTCCTCAGGACAAGCATCTC TTCACTGTCCTGGTGCAGGAAATGCGTGAAGCTTTTGAGCAGGAGGCCAAGCAGATCAACAAGCCCAGG CTGATGGTCACTGCTGCAGTAGCTGCTGGCATCTCCAATATCCAGTCTGGCTATGAGATCCCCCAACTG TCACAGTACCTGGACTACATCCATGTCATGACCTACGACCTCCATGGCTCCTGGGAGGGCTACACTGGA GAGAACAGCCCCCTCTACAAATACCCGACTGACACCGGCAGCAACGCCTACCTCAATGTGGATTATGTC ATGAACTACTGGAAGGACAATGGAGCACCAGCTGAGAAGCTCATCGTTGGATTCCCTACCTATGGACAC AACTTCATCCTGAGCAACCCCTCCAACACTGGAATTGGTGCCCCCACCTCTGGTGCTGGTCCTGCTGGG CCCTATGCCAAGGAGTCTGGGATCTGGGCTTACTACGAGATCTGTACCTTCCTGAAAAATGGAGCCACT CAGGGATGGGATGCCCCTCAGGAAGTGCCTTATGCCTATCAGGGCAATGTGTGGGTTGGCTATGACAAC ATCAAGAGCTTCGATATTAAGGCTCAATGGCTTAAGCACAACAAATTTGGAGGCGCCATGGTCTGGGCC ATTGATCTGGATGACTTCACTGGCACTTTCTGCAACCAGGGCAAGTTTCCCCTAATCTCCACCCTGAAG AAGGCCCTCGGCCTGCAGAGTGCAAGTTGCACGGCTCCAGCTCAGCCCATTGAGCCAATAACTGCTGCT CCCAGTGGCAGCGGGAACGGGAGCGGGAGTAGCAGCTCTGGAGGCAGCTCGGGAGGCAGTGGATTCTGT GCTGTCAGAGCCAACGGCCTCTACCCCGTGGCAAATAACAGAAATGCCTTCTGGCACTGCGTGAATGGA GTCACGTACCAGCAGAACTGCCAGGCCGGGCTTGTCTTCGACACCAGCTGTGATTGCTGCAACTGGGCA TAA
Restriction Sites	SgfI-MluI
ACCN	NM_001258001
Insert Size	1107 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001258001.1
RefSeq Size	1537 bp
RefSeq ORF	1107 bp
Locus ID	27159
UniProt ID	Q9BZP6
Protein Families	Secreted Protein
Protein Pathways	Amino sugar and nucleotide sugar metabolism
MW	40.1 kDa
Gene Summary	The protein encoded by this gene degrades chitin, which is found in the cell wall of most fungi as well as in arthropods and some nematodes. The encoded protein can also stimulate interleukin 13 expression, and variations in this gene can lead to asthma susceptibility. Several transcript variants encoding a few different isoforms have been found for this gene. [provided by RefSeq, Apr 2012] Transcript Variant: This variant (5) lacks an exon in the 5' coding region and initiates translation at a downstream AUG compared to variant 4. The encoded isoform (a, also known as TSA1902-L) has a shorter N-terminus and lacks a signal peptide compared to isoform c. Variants 2, 5, and 7 all encode the same isoform (a).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.