ITCH (NM_001257138) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ADMFD; AIF4; AIP4; NAPP1
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332505 representing NM_001257138. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGAAACTTGAAGAAGTAGTTGTGACTTTGCAGCTTGGAGGTGACAAAGAGCCAACAGAGACAATAGGA GACTTGTCAATTTGTCTTGATGGGCTACAGTTAGAGTCTGAAGTTGTTACCAATGGTGAAACTACATGT TCAGAAAGTGCTTCTCAGAATGATGATGGCTCCAGATCCAAGGATGAAACAAGAGTGAGCACAAATGGA TCAGATGACCCTGAAGATGCAGGAGCTGGTGAAAATAGGAGAGTCAGTGGGAATAATTCTCCATCACTC TCAAATGGTGGTTTTAAACCTTCTAGACCTCCAAGACCTTCACGACCACCACCACCCACCCCACGTAGA CCAGCATCTGTCAATGGTTCACCATCTGCCACTTCTGAAAGTGATGGGTCTAGTACAGGCTCTCTGCCG CCGACAAATACAAATACAAATACATCTGAAGGAGCAACATCTGGATTAATAATTCCTCTTACTATATCT GGAGGCTCAGGCCCTAGGCCATTAAATCCTGTAACTCAAGCTCCCTTGCCACCTGGTTGGGAGCAGAGA GTGGACCAGCACGGGCGAGTTTACTATGTAGATCATGTTGAGAAAAGAACAACATGGGATAGACCAGAA CCTCTACCTCCTGGCTGGGAACGGCGGGTTGACAACATGGGACGTATTTATTATGTTGACCATTTCACA AGAACAACAACGTGGCAGAGGCCAACACTGGAATCCGTCCGGAACTATGAACAATGGCAGCTACAGCGT AGTCAGCTTCAAGGAGCAATGCAGCAGTTTAACCAGAGATTCATTTATGGGAATCAAGATTTATTTGCT ACATCACAAAGTAAAGAATTTGATCCTCTTGGTCCATTGCCACCTGGATGGGAGAAGAGAACAGACAGC AATGGCAGAGTATATTTCGTCAACCACAACACACGAATTACACAATGGGAAGACCCCAGAAGTCAAGGT CAATTAAATGAAAAGCCCTTACCTGAAGGTTGGGAAATGAGATTCACAGTGGATGGAATTCCATATTTT GTGGACCACAATAGAAGAACTACCACCTATATAGATCCCCGCACAGGAAAATCTGCCCTAGACAATGGA CCTCAGATAGCCTATGTTCGGGACTTCAAAGCAAAGGTTCAGTATTTCCGGTTCTGGTGTCAGCAACTG GCCATGCCACAGCACATAAAGATTACAGTGACAAGAAAAACATTGTTTGAGGATTCCTTTCAACAGATA ATGAGCTTCAGTCCCCAAGATCTGCGAAGACGTTTGTGGGTGATTTTTCCAGGAGAAGAAGGTTTAGAT TATGGAGGTGTAGCAAGAGAATGGTTCTTTCTTTTGTCACATGAAGTGTTGAACCCAATGTATTGCCTG TTTGAATATGCAGGGAAGGATAACTACTGCTTGCAGATAAACCCCGCTTCTTACATCAATCCAGATCAC CTGAAATATTTTCGTTTTATTGGCAGATTTATTGCCATGGCTCTGTTCCATGGGAAATTCATAGACACG GGTTTTTCTTTACCATTCTATAAGCGTATCTTGAACAAACCAGTTGGACTCAAGGATTTAGAATCTATT GATCCAGAATTTTACAATTCTCTCATCTGGGTTAAGGAAAACAATATTGAGGAATGTGATTTGGAAATG TACTTCTCCGTTGACAAAGAAATTCTAGGTGAAATTAAGAGTCATGATCTGAAACCTAATGGTGGCAAT ATTCTTGTAACAGAAGAAAATAAAGAGGAATACATCAGAATGGTAGCTGAGTGGAGGTTGTCTCGAGGT GTTGAAGAACAGACACAAGCTTTCTTTGAAGGCTTTAATGAAATTCTTCCCCAGCAATATTTGCAATAC TTTGATGCAAAGGAATTAGAGGTCCTTTTATGTGGAATGCAAGAGATTGATTTGAATGACTGGCAAAGA CATGCCATCTACCGTCATTATGCAAGGACCAGCAAACAAATCATGTGGTTTTGGCAGTTTGTTAAAGAA ATTGATAATGAGAAGAGAATGAGACTTCTGCAGTTTGTTACTGGAACCTGCCGATTGCCAGTAGGAGGA TTTGCTGATCTCATGGGGAGCAATGGACCACAGAAATTCTGCATTGAAAAAGTTGGGAAAGAAAATTGG CTACCCAGAAGTCATACCTGTTTTAATCGCCTGGACCTGCCACCATACAAGAGCTATGAGCAACTGAAG GAAAAGCTGTTGTTTGCCATAGAAGAAACAGAAGGATTTGGACAAGAGTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001257138
Insert Size	2259 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001257138.1
RefSeq Size	6270 bp
RefSeq ORF	2259 bp
Locus ID	83737
UniProt ID	Q96J02
Protein Families	Druggable Genome, Transcription Factors
Protein Pathways	Endocytosis, Ubiquitin mediated proteolysis
MW	86.5 kDa
Gene Summary	This gene encodes a member of the Nedd4 family of HECT domain E3 ubiquitin ligases. HECT domain E3 ubiquitin ligases transfer ubiquitin from E2 ubiquitin-conjugating enzymes to protein substrates, thus targeting specific proteins for lysosomal degradation. The encoded protein plays a role in multiple cellular processes including erythroid and lymphoid cell differentiation and the regulation of immune responses. Mutations in this gene are a cause of syndromic multisystem autoimmune disease. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Mar 2012] Transcript Variant: This variant (3) lacks two internal exons and initiates translation at a downstream, in-frame start codon, compared to variant 1. The encoded isoform (3) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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