ITCH (NM_001257137) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ADMFD; AIF4; AIP4; NAPP1
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332504 representing NM_001257137. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGTCTGACAGTGGATCACAACTTGGTTCAATGGGTAGCCTCACCATGAAATCACAGCTTCAGATCACT GTCATCTCAGCAAAACTTAAGGAAAATAAGAAGAATTGGTTTGGACCAAGTCCTTACGTAGAGGTCACA GTAGATGGACAGTCAAAGAAGACAGAAAAATGCAACAACACAAACAGTCCCAAGTGGAAGCAACCCCTT ACAGTTATCGTTACCCCTGTGAGTAAATTACATTTTCGTGTGTGGAGTCACCAGACACTGAAATCTGAT GTTTTGTTGGGAACTGCTGCATTAGATATTTATGAAACATTAAAGTCAAACAATATGAAACTTGAAGAA GTAGTTGTGACTTTGCAGCTTGGAGGTGACAAAGAGCCAACAGAGACAATAGGAGACTTGTCAATTTGT CTTGATGGGCTACAGTTAGAGTCTGAAGTTGTTACCAATGGTGAAACTACATGTTCAGAAAATGGGGTT TCGCTCTGTTTGCCCAGGCTGGAGTGCAATAGTGCGATCTCGGCTCACTGCAACCTCTGCCTCCCGGGT TTAAGTGATTCTCCTATCTCAGCCTCCCGAGTAGCTGGGTTTACAGGTGCTTCTCAGAATGATGATGGC TCCAGATCCAAGGATGAAACAAGAGTGAGCACAAATGGATCAGATGACCCTGAAGATGCAGGAGCTGGT GAAAATAGGAGAGTCAGTGGGAATAATTCTCCATCACTCTCAAATGGTGGTTTTAAACCTTCTAGACCT CCAAGACCTTCACGACCACCACCACCCACCCCACGTAGACCAGCATCTGTCAATGGTTCACCATCTGCC ACTTCTGAAAGTGATGGGTCTAGTACAGGCTCTCTGCCGCCGACAAATACAAATACAAATACATCTGAA GGAGCAACATCTGGATTAATAATTCCTCTTACTATATCTGGAGGCTCAGGCCCTAGGCCATTAAATCCT GTAACTCAAGCTCCCTTGCCACCTGGTTGGGAGCAGAGAGTGGACCAGCACGGGCGAGTTTACTATGTA GATCATGTTGAGAAAAGAACAACATGGGATAGACCAGAACCTCTACCTCCTGGCTGGGAACGGCGGGTT GACAACATGGGACGTATTTATTATGTTGACCATTTCACAAGAACAACAACGTGGCAGAGGCCAACACTG GAATCCGTCCGGAACTATGAACAATGGCAGCTACAGCGTAGTCAGCTTCAAGGAGCAATGCAGCAGTTT AACCAGAGATTCATTTATGGGAATCAAGATTTATTTGCTACATCACAAAGTAAAGAATTTGATCCTCTT GGTCCATTGCCACCTGGATGGGAGAAGAGAACAGACAGCAATGGCAGAGTATATTTCGTCAACCACAAC ACACGAATTACACAATGGGAAGACCCCAGAAGTCAAGGTCAATTAAATGAAAAGCCCTTACCTGAAGGT TGGGAAATGAGATTCACAGTGGATGGAATTCCATATTTTGTGGACCACAATAGAAGAACTACCACCTAT ATAGATCCCCGCACAGGAAAATCTGCCCTAGACAATGGACCTCAGATAGCCTATGTTCGGGACTTCAAA GCAAAGGTTCAGTATTTCCGGTTCTGGTGTCAGCAACTGGCCATGCCACAGCACATAAAGATTACAGTG ACAAGAAAAACATTGTTTGAGGATTCCTTTCAACAGATAATGAGCTTCAGTCCCCAAGATCTGCGAAGA CGTTTGTGGGTGATTTTTCCAGGAGAAGAAGGTTTAGATTATGGAGGTGTAGCAAGAGAATGGTTCTTT CTTTTGTCACATGAAGTGTTGAACCCAATGTATTGCCTGTTTGAATATGCAGGGAAGGATAACTACTGC TTGCAGATAAACCCCGCTTCTTACATCAATCCAGATCACCTGAAATATTTTCGTTTTATTGGCAGATTT ATTGCCATGGCTCTGTTCCATGGGAAATTCATAGACACGGGTTTTTCTTTACCATTCTATAAGCGTATC TTGAACAAACCAGTTGGACTCAAGGATTTAGAATCTATTGATCCAGAATTTTACAATTCTCTCATCTGG GTTAAGGAAAACAATATTGAGGAATGTGATTTGGAAATGTACTTCTCCGTTGACAAAGAAATTCTAGGT GAAATTAAGAGTCATGATCTGAAACCTAATGGTGGCAATATTCTTGTAACAGAAGAAAATAAAGAGGAA TACATCAGAATGGTAGCTGAGTGGAGGTTGTCTCGAGGTGTTGAAGAACAGACACAAGCTTTCTTTGAA GGCTTTAATGAAATTCTTCCCCAGCAATATTTGCAATACTTTGATGCAAAGGAATTAGAGGTCCTTTTA TGTGGAATGCAAGAGATTGATTTGAATGACTGGCAAAGACATGCCATCTACCGTCATTATGCAAGGACC AGCAAACAAATCATGTGGTTTTGGCAGTTTGTTAAAGAAATTGATAATGAGAAGAGAATGAGACTTCTG CAGTTTGTTACTGGAACCTGCCGATTGCCAGTAGGAGGATTTGCTGATCTCATGGGGAGCAATGGACCA CAGAAATTCTGCATTGAAAAAGTTGGGAAAGAAAATTGGCTACCCAGAAGTCATACCTGTTTTAATCGC CTGGACCTGCCACCATACAAGAGCTATGAGCAACTGAAGGAAAAGCTGTTGTTTGCCATAGAAGAAACA GAAGGATTTGGACAAGAGTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001257137
Insert Size	2712 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001257137.1
RefSeq Size	6535 bp
RefSeq ORF	2712 bp
Locus ID	83737
UniProt ID	Q96J02
Protein Families	Druggable Genome, Transcription Factors
Protein Pathways	Endocytosis, Ubiquitin mediated proteolysis
MW	102.8 kDa
Gene Summary	This gene encodes a member of the Nedd4 family of HECT domain E3 ubiquitin ligases. HECT domain E3 ubiquitin ligases transfer ubiquitin from E2 ubiquitin-conjugating enzymes to protein substrates, thus targeting specific proteins for lysosomal degradation. The encoded protein plays a role in multiple cellular processes including erythroid and lymphoid cell differentiation and the regulation of immune responses. Mutations in this gene are a cause of syndromic multisystem autoimmune disease. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Mar 2012] Transcript Variant: This variant (1) encodes the longest isoform (1). Both variants 1 and 4 encode the same isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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