CLCN6 (NM_001256959) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CLC-6; CONRIBA
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332500 representing NM_001256959. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGCGGGGTGCAGGGGGTCTCTGTGCTGCTGCTGCAGGTGGTGCTGCTGCTGCGGTGAGCGTGAGACC CGCACCCCCGAGGAGCTGACCATCCTTGGAGAAACACAGGAGGAGGAGGATGAGATTCTTCCAAGGAAA GACTATGAGAAAGGTCGAAGATATGAGGCGGTGAAGTGGATGGTGGTGTTTGCCATTGGAGTCTGCACT GGCCTGGTGGGTCTCTTTGTGGACTTTTTTGTGCGACTCTTCACCCAACTCAAGTTCGGAGTGGTACAG ACATCGGTGGAGGAGTGCAGCCAGAAAGGCTGCCTCGCTCTGTCTCTCCTTGAACTCCTGGGTTTTAAC CTCACCTTTGTCTTCCTGGCAAGCCTCCTTGTTCTCATTGAGCCGGTGGCAGCAGGTTCCGGGATACCC GAGGTCAAATGCTATCTGAATGGCGTAAAGGTGCCAGGAATCGTCCGTCTCCGGACCCTGCTCTGCAAG GTCCTTGGAGTGCTGTTCAGTGTGGCTGGAGGGCTCTTCGTGGGGAAGGAAGGCCCCATGATCCACAGT GGTTCGGTGGTGGGAGCTGGCCTCCCTCAGTTTCAGAGCATCTCCTTACGGAAGATCCAGTTTAACTTC CCCTATTTCCGAAGCGACAGAGACAAGAGAGACTTTGTATCAGCAGGAGCGGCTGCTGGAGTTGCTGCA GCTTTCGGGGCGCCAATCGGGGGTACCTTGTTCAGTCTAGAGGAGGGTTCGTCCTTCTGGAACCAAGGG CTCACGTGGAAAGTGCTCTTTTGTTCCATGTCTGCCACCTTCACCCTCAACTTCTTCCGTTCTGGGATT CAGTTTGGAAGCTGGGGTTCCTTCCAGCTCCCTGGATTGCTGAACTTTGGCGAGTTTAAGTGCTCTGAC TCTGATAAAAAATGTCATCTCTGGACAGCTATGGATTTGGGTTTCTTCGTCGTGATGGGGGTCATTGGG GGCCTCCTGGGAGCCACATTCAACTGTCTGAACAAGAGGCTTGCAAAGTACCGTATGCGAAACGTGCAC CCGAAACCTAAGCTCGTCAGAGTCTTAGAGAGCCTCCTTGTGTCTCTGGTAACCACCGTGGTGGTGTTT GTGGCCTCGATGGTGTTAGGAGAATGCCGACAGATGTCCTCTTCGAGTCAAATCGGTAATGACTCATTC CAGCTCCAGGTCACAGAAGATGTGAATTCAAGTATCAAGACATTTTTTTGTCCCAATGATACCTACAAT GACATGGCCACACTCTTCTTCAACCCGCAGGAGTCTGCCATCCTCCAGCTCTTCCACCAGGATGGTACT TTCAGCCCCGTCACTCTGGCCTTGTTCTTCGTTCTCTATTTCTTGCTTGCATGTTGGACTTACGGCATT TCTGTTCCAAGTGGCCTTTTTGTGCCTTCTCTGCTGTGTGGAGCTGCTTTTGGACGTTTAGTTGCCAAT GTCCTAAAAAGCTACATTGGATTGGGCCACATCTATTCGGGGACCTTTGCCCTGATTGGTGCAGCGGCT TTCTTGGGCGGGGTGGTCCGCATGACCATCAGCCTCACGGTCATCCTGATCGAGTCCACCAATGAGATC ACCTACGGGCTCCCCATCATGGTCACACTGATGGTGGCCAAATGGACAGGGGACTTTTTCAATAAGGGC ATTTATGATATCCACGTGGGCCTGCGAGGCGTGCCGCTTCTGGAATGGGAGACAGAGGTGGAAATGGAC AAGCTGAGAGCCAGCGACATCATGGAGCCCAACCTGACCTACGTCTACCCGCACACCCGCATCCAGTCT CTGGTGAGCATCCTGCGCACCACGGTCCACCATGCCTTCCCGGTGGTCACAGAGAACCGCGGTAACGAG AAGGAGTTCATGAAGGGCAACCAGCTCATCAGCAACAACATCAAGTTCAAGAAATCCAGCATCCTCACC CGGGCTGGCGAGCAGCGCAAACGGAGCCAGTCCATGAAGTCCTACCCATCCAGCGAGCTACGGAACATG TGTGATGAGCACATCGCCTCTGAGGAGCCAGCCGAGAAGGAGGACCTCCTGCAGCAGATGCTGGAAAGG AGATACACTCCCTACCCCAACCTATACCCTGACCAGTCCCCAAGTGAAGACTGGACCATGGAGGAGCGG TTCCGCCCTCTGACCTTCCACGGCCTGATCCTTCGGTCGCAGCTTGTCACCCTGCTTGTCCGAGGAGTT TGTTACTCTGAAAGCCAGTCGAGCGCCAGCCAGCCGCGCCTCTCCTATGCCGAGATGGCCGAGGACTAC CCGCGGTACCCCGACATCCACGACCTGGACCTGACGCTGCTCAACCCGCGCATGATCGTGGATGTCACC CCATACATGAACCCTTCGCCTTTCACCGTCTCGCCCAACACCCACGTCTCCCAAGTCTTCAACCTGTTC AGAACGATGGGCCTGCGCCACCTGCCCGTGGTGAACGCTGTGGGAGAGATCGTGGGGATCATCACACGG CACAACCTCACCTATGAATTTCTGCAGGCCCGGCTGAGGCAGCACTACCAGACCATCTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001256959
Insert Size	2544 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001256959.1
RefSeq Size	5647 bp
RefSeq ORF	2544 bp
Locus ID	1185
UniProt ID	P51797
Protein Families	Druggable Genome, Ion Channels: Other, Transmembrane
MW	94.6 kDa
Gene Summary	This gene encodes a member of the voltage-dependent chloride channel protein family. Members of this family can function as either chloride channels or antiporters. This protein is primarily localized to late endosomes and functions as a chloride/proton antiporter. Alternate splicing results in both coding and non-coding variants. Additional alternately spliced variants have been described but their full-length structure is unknown. [provided by RefSeq, Mar 2012] Transcript Variant: This variant (2) lacks an in-frame exon in the coding region, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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