CLCN4 (NM_001256944) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ClC-4; ClC-4A; CLC4; MRX15; MRX49; MRXSRC
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332498 representing NM_001256944. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGACGGACCTGAAGGAGGGGGTCTGCCTGTCTGCCTTCTGGTATAGCCATGAGCAGTGTTGCTGGACT TCTAACGAGACCACTTTTGAGGACAGAGACAAGTGTCCCCTGTGGCAGAAATGGTCGGAGCTGCTGGTG AATCAGTCAGAGGGTGCCAGTGCTTACATTCTGAATTACTTAATGTACATCCTATGGGCGCTGCTGTTT GCATTTTTGGCTGTCTCCCTGGTGCGTGTATTTGCACCATATGCCTGTGGCTCTGGCATACCAGAGATA AAGACCATTTTGAGCGGCTTTATCATCAGGGGCTACTTGGGGAAGTGGACCCTGCTAATCAAGACAGTC ACGCTGGTGCTGGTAGTGTCCTCCGGTCTGAGCCTTGGGAAGGAAGGGCCGCTAGTGCACGTGGCTTGT TGCTGTGGCAACTTCTTCAGCAGCCTTTTCTCCAAGTACAGCAAGAATGAGGGCAAGAGGCGGGAGGTG CTTTCAGCTGCAGCGGCTGCTGGAGTCTCTGTTGCCTTTGGTGCACCAATTGGAGGCGTGCTTTTCAGT CTAGAAGAGGTCAGTTACTACTTTCCCCTGAAGACCTTGTGGAGGTCATTTTTCGCAGCCCTGGTGGCG GCCTTTACGCTGAGATCCATCAATCCCTTTGGGAATAGCCGTCTCGTTCTCTTTTATGTGGAATACCAC ACGCCCTGGTACATGGCTGAACTCTTCCCCTTCATCCTGCTTGGGGTCTTCGGGGGCTTGTGGGGAACC CTCTTCATCCGCTGCAACATCGCCTGGTGCAGGAGGCGCAAGACCACCAGGCTGGGGAAGTACCCGGTG CTGGAGGTCATTGTGGTGACTGCCATCACTGCCATCATTGCCTACCCCAATCCCTACACACGCCAGAGC ACCAGCGAGCTCATTTCTGAGCTGTTCAATGACTGTGGAGCCCTTGAGTCTTCCCAGCTCTGTGACTAC ATCAATGACCCCAACATGACTCGGCCTGTGGATGACATTCCAGACCGGCCGGCTGGTGTCGGTGTTTAC ACGGCCATGTGGCAGCTGGCCCTGGCACTGATCTTCAAAATCGTCGTTACCATATTTACCTTTGGCATG AAGATCCCGTCGGGCCTCTTCATCCCCAGCATGGCTGTGGGCGCGATAGCGGGCAGGATGGTGGGAATT GGCGTGGAGCAGCTGGCCTACCATCACCATGACTGGATCATCTTCAGGAACTGGTGCAGACCCGGTGCA GACTGTGTCACGCCAGGGCTGTACGCAATGGTGGGAGCTGCGGCCTGCCTCGGTGGAGTTACCAGGATG ACGGTGTCATTGGTGGTCATCATGTTTGAATTAACCGGGGGTCTGGAGTACATCGTGCCCCTGATGGCG GCGGCTGTGACCAGCAAGTGGGTAGCTGATGCATTTGGGAAAGAAGGCATCTACGAGGCCCACATCCAC TTAAATGGGTACCCTTTCCTTGACGTGAAGGACGAGTTTACTCACCGCACACTGGCCACCGACGTCATG CGGCCCCGGCGGGGAGAGCCGCCACTGTCGGTGCTCACCCAGGACAGCATGACTGTCGAGGACGTGGAG ACGCTCATCAAGGAGACCGACTACAACGGCTTCCCCGTGGTGGTCTCCAGAGACTCCGAGCGCCTCATT GGATTTGCCCAGAGGAGGGAACTGATTCTCGCAATAAAGAACGCCAGACAGAGGCAGGAGGGCATTGTG AGCAATTCCATCATGTACTTCACGGAGGAACCCCCCGAGCTGCCGGCCAACAGCCCACATCCCCTGAAG CTGCGGCGCATCCTGAACCTCAGCCCGTTTACAGTGACAGACCACACTCCGATGGAAACGGTGGTGGAT ATCTTCCGGAAACTGGGGCTTCGGCAGTGCCTGGTGACGCGGAGCGGGAGACTTCTTGGCATCATCACA AAAAAGGATGTTCTGAGACATATGGCCCAGATGGCAAACCAGGACCCCGAATCCATCATGTTTAATTAG
Restriction Sites	SgfI-MluI
ACCN	NM_001256944
Insert Size	2001 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001256944.1
RefSeq Size	6534 bp
RefSeq ORF	2001 bp
Locus ID	1183
UniProt ID	P51793
Protein Families	Druggable Genome, Ion Channels: Other, Transmembrane
MW	74.3 kDa
Gene Summary	The CLCN family of voltage-dependent chloride channel genes comprises nine members (CLCN1-7, Ka and Kb) which demonstrate quite diverse functional characteristics while sharing significant sequence homology. Chloride channel 4 has an evolutionary conserved CpG island and is conserved in both mouse and hamster. This gene is mapped in close proximity to APXL (Apical protein Xenopus laevis-like) and OA1 (Ocular albinism type I), which are both located on the human X chromosome at band p22.3. The physiological role of chloride channel 4 remains unknown but may contribute to the pathogenesis of neuronal disorders. Alternate splicing results in two transcript variants that encode different proteins. [provided by RefSeq, Mar 2012] Transcript Variant: This variant (2) differs in the 5' UTR and lacks an in-frame portion of the 5' coding region, compared to variant 1. The resulting isoform (2) has a shorter N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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