CNOT10 (NM_001256742) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332460 representing NM_001256742. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGAAGTCTATTGGTGTAAAGACTGTCAAGGTTTGTGGGTCAAAGAGCTCTGTACGCATAGAAGGTCAG CCATGTTCATTAGGCCAGAGTAGAAGGAAAGTCAAGCTTATGGGTGAACGGTCGTATACTCTTTCAATT GGCAACGGAGACTACTTTTGGGCAAATAAAGAAATGCTGTGGGATTATGTGCAGACTCTGTCTGATCAG GGAGCAGAGAAACATGAAGGCACAGGTCAGTCCTCTGGGATCACTGATCAAGAGAAGGAGTTATCCACC AATGCTTTCCAAGCTTTCACATCTGGAAATTATGATGCCTGTCTACAACACCTTGCCTGTCTACAAGAT ATAAACAAAGATGATTATAAAATAATTTTGAATACAGCAGTAGCTGAGTTTTTTAAAAGTAACCAAACA ACAACAGATAATTTGAGACAAACACTTAACCAGCTGAAGAATCAGGTCCACTCAGCTGTTGAAGAAATG GATGGATTAGATGATGTTGAAAACAGCATGTTGTACTATAATCAAGCAGTCATTCTTTATCATCTGCGG CAGTATACAGAAGCCATATCAGTTGGTGAAAAACTTTATCAGTTCATAGAGCCTTTTGAAGAAAAATTT GCCCAAGCAGTGTGTTTTTTGCTTGTAGACCTGTATATATTAACCTACCAAGCTGAGAAAGCTTTGCAT CTTCTTGCTGTCCTAGAAAAAATGATTTCACAGGGTAACAATAACAAAAATGGAAAGAATGAGACTGGT AATAACAACAACAAAGATGGATCTAATCATAAAGCTGAAAGTGGAGCTCTAATAGAAGCTGCAAAATCA AAGATACATCAGTACAAAGTACGAGCTTATATCCAAATGAAGTCTCTGAAAGCATGTAAAAGGGAAATC AAGTCAGTCATGAATACAGCTGGAAATTCCGCACCCTCTCTCTTTCTTAAAAGCAATTTTGAGTACTTA AGAGGTAATTATCGAAAAGCCGTGAAGCTATTAAATAGTTCAAACATTGCTGAGCATCCAGGATTCATG AAAACAGGTGAATGCTTGAGATGCATGTTCTGGAATAACCTTGGTTGCATCCATTTTGCCATGAGCAAG CACAATTTGGGAATATTCTACTTTAAAAAGGCTCTGCAAGAGAATGACAATGTCTGTGCACAGCTCAGT GCAGGTAGCACTGATCCAGGTAAAAAATTTTCAGGAAGACCCATGTGTACGTTACTAACCAATAAGAGA TATGAGTTGCTGTATAACTGTGGAATTCAGCTTCTTCACATTGGAAGGCCTCTTGCTGCCTTCGAATGT CTGATTGAAGCTGTTCAGGTTTATCATGCAAATCCTCGCCTCTGGCTACGGCTGGCTGAATGCTGCATT GCTGCCAATAAGGGGACTTCTGAACAAGAAACTAAAGGCCTTCCCAGCAAAAAAGGAATTGTACAGTCT ATTGTTGGTCAAGGCTATCATCGTAAAATAGTTTTGGCATCACAGTCTATACAGAATACTGTTTATAAT GATGGGCAGTCTTCGGCCATTCCTGTAGCCAGTATGGAGTTTGCAGCCATATGTCTCAGAAATGCCTTG TTGCTGCTACCTGAAGAACAGCAAGATCCAAAGCAGGAAAATGGGGCTAAAAATAGTAATCAATTAGGT GGGAACACAGAGAGCAGCGAAAGCAGTGAAACTTGCAGCAGTAAAAGCCATGATGGAGATAAATTCATT CCAGCTCCACCTTCTTCTCCATTGAGAAAACAGGAATTAGAAAACTTAAAGTGCTCCATACTTGCTTGC AGTGCCTACGTGGCTCTGGCTTTGGGTGATAACCTCATGGCTTTGAATCATGCAGATAAACTTCTTCAG CAGCCCAAGCTGTCAGGATCTCTTAAGTTTTTGGGACATTTATATGCTGCAGAAGCCCTCATCTCTCTC GACAGAATATCTGATGCCATTACTCACTTGAACCCGGAGAATGTCACTGATGTCTCCTTAGGGATCTCT TCAAATGAGCAGGACCAAGGATCAGACAAAGGTGAAAATGAAGCAATGGAATCCTCTGGTAAGCGGGCC CCTCAGTGCTACCCCAGTTCCGTCAACTCTGCCAGGACTGTGATGCTGTTCAACCTTGGCAGCGCTTAC TGCCTGAGGAGCGAATATGACAAAGCCCGAAAGTGTCTCCACCAGGCGGCTTCAATGATCCATCCTAAA GAGGTGCCCCCTGAGGCCATCTTGCTGGCAGTCTACCTTGAACTGCAGAATGGTAATACTCAGCTGGCC TTACAGATCATCAAAAGGAATCAGCTGCTCCCTGCAGTGAAAACACACTCTGAAGTGAGAAAGAAGCCA GTGTTTCAGCCTGTCCACCCGATCCAGCCCATCCAAATGCCGGCTTTCACCACTGTGCAGAGAAAGTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001256742
Insert Size	2415 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001256742.1
RefSeq Size	2778 bp
RefSeq ORF	2415 bp
Locus ID	25904
UniProt ID	Q9H9A5
Protein Pathways	RNA degradation
MW	89.1 kDa
Gene Summary	Component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. Additional complex functions may be a consequence of its influence on mRNA expression. Is not required for association of CNOT7 to the CCR4-NOT complex.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) differs in the 5' UTR, lacks a portion of the 5' coding region and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (3) is longer and has a distinct N-terminus, compared to isoform 1.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.