PRMT8 (NM_001256536) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	HRMT1L3; HRMT1L4
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332413 representing NM_001256536. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGAGTCTCTGGCTTCAGATGGATTCAAGCTGAAAGAGGTTTCTTCTGTGAACAGCCCCCCCTCCCAG CCCCCCCAGCCCGTCGTCCCTGCTAAGCCCGTGCAATGCGTCCATCATGTGTCCACTCAACCCAGCTGC CCAGGACGGGGCAAGATGTCCAAGCTGCTGAACCCAGAGGAGATGACCTCGAGAGATTATTACTTCGAC TCCTATGCCCACTTTGGGATCCACGAGGAAATGCTGAAGGATGAGGTGCGGACTCTCACTTACCGGAAC TCCATGTACCACAACAAGCACGTGTTCAAGGACAAAGTGGTACTGGATGTGGGGAGTGGTACTGGGATC CTTTCCATGTTCGCTGCCAAGGCAGGGGCCAAGAAGGTGTTTGGGATCGAATGCTCCAGTATTTCTGAC TACTCAGAGAAGATCATTAAGGCCAACCACTTGGACAACATCATCACCATATTTAAGGGTAAAGTGGAA GAGGTGGAGCTGCCTGTGGAGAAGGTGGACATCATCATCAGCGAGTGGATGGGCTACTGTCTGTTCTAT GAGTCCATGCTCAACACGGTGATCTTTGCCAGGGACAAGTGGCTGAAACCTGGAGGGCTTATGTTTCCA GACCGGGCAGCTTTGTACGTGGTAGCGATTGAAGACAGACAGTACAAGGACTTCAAAATCCACTGGTGG GAGAATGTCTATGGCTTTGACATGACCTGCATCCGGGACGTGGCCATGAAGGAGCCTCTAGTGGACATC GTGGATCCAAAGCAAGTGGTGACCAATGCCTGTTTGATAAAGGAGGTGGACATTTACACAGTGAAGACG GAAGAGCTATCGTTCACATCTGCATTCTGCCTGCAGATACAGCGCAACGACTACGTCCACGCCCTGGTC ACCTATTTTAATATTGAATTTACCAAGTGCCACAAGAAAATGGGGTTTTCCACAGCCCCTGATGCTCCC TACACCCACTGGAAGCAGACCGTCTTCTACTTGGAAGATTACCTCACTGTCCGGAGGGGGGAGGAAATC TACGGGACCATATCCATGAAGCCAAATGCCAAAAATGTGCGAGACCTCGATTTCACAGTAGACTTGGAT TTTAAGGGACAGCTGTGTGAAACATCTGTATCTAATGACTACAAAATGCGTTAG
Restriction Sites	SgfI-MluI
ACCN	NM_001256536
Insert Size	1158 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001256536.1
RefSeq Size	1995 bp
RefSeq ORF	1158 bp
Locus ID	56341
UniProt ID	Q9NR22
Protein Families	Druggable Genome
MW	44.2 kDa
Gene Summary	Arginine methylation is a widespread posttranslational modification mediated by arginine methyltransferases, such as PRMT8. Arginine methylation is involved in a number of cellular processes, including DNA repair, RNA transcription, signal transduction, protein compartmentalization, and possibly protein translation (Lee et al., 2005 [PubMed 16051612]).[supplied by OMIM, Mar 2008] Transcript Variant: This variant (2) uses an alternate 5' exon and it thus differs in the 5' UTR and 5' coding region, compared to variant 1. The resulting isoform (2) has a distinct N-terminus and is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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