ThPok (ZBTB7B) (NM_001256455) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	c-KROX; CKROX; hcKROX; THPOK; ZBTB15; ZFP-67; ZFP67; ZNF857B
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332388 representing NM_001256455. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGGGAGCCCCGAGGATGACCTGATTGGGATTCCATTCCCGGACCACAGCAGTGAGCTCCTGAGCTGC CTCAATGAGCAGCGCCAGCTGGGCCACCTATGTGACCTCACCATCCGGACGCAGGGCCTTGAATACCGC ACCCACAGGGCTGTGCTAGCTGCCTGTAGCCACTACTTCAAGAAGCTTTTCACTGAGGGCGGTGGCGGA GCTGTCATGGGGGCCGGGGGTAGCGGGACGGCCACTGGGGGAGCAGGGGCCGGTGTGTGTGAGCTGGAC TTTGTAGGGCCAGAGGCACTAGGCGCCCTCCTTGAATTTGCCTATACAGCCACACTGACCACCAGCAGC GCCAACATGCCAGCTGTGCTCCAGGCTGCCCGCCTGCTGGAGATCCCGTGTGTCATCGCTGCTTGCATG GAGATTCTGCAGGGCAGTGGGCTAGAAGCTCCCAGCCCGGACGAGGATGACTGTGAGCGAGCCCGCCAG TATCTGGAGGCCTTTGCCACAGCCACGGCCTCTGGAGTTCCCAATGGTGAAGACAGTCCTCCACAGGTG CCCCTCCCACCACCTCCGCCACCGCCACCTCGGCCTGTTGCCCGCCGCAGCCGCAAGCCCCGGAAAGCT TTCCTGCAAACCAAGGGGGCCAGAGCAAACCACCTAGTCCCTGAGGTGCCCACAGTGCCCGCCCATCCC TTGACCTATGAGGAGGAGGAGGTGGCGGGCAGAGTGGGCAGCAGTGGGGGCAGTGGGCCGGGGGACAGC TACAGCCCTCCCACAGGAACTGCCTCCCCTCCTGAGGGTCCCCAGAGCTACGAACCCTATGAGGGTGAG GAAGAAGAAGAGGAGCTGGTATATCCCCCAGCCTATGGGCTGGCGCAGGGTGGCGGGCCCCCGCTGTCC CCAGAGGAGCTGGGCTCAGATGAGGATGCCATCGATCCTGACCTGATGGCCTACCTAAGCTCCCTGCAC CAGGACAACCTGGCACCAGGCCTGGACAGCCAAGACAAGCTGGTGCGCAAACGCCGCTCCCAGATGCCT CAGGAGTGCCCTGTCTGCCACAAGATCATCCATGGGGCAGGCAAACTGCCTCGCCACATGAGGACCCAC ACAGGCGAGAAGCCCTTTGCCTGCGAGGTCTGCGGTGTTCGATTCACCAGGAACGACAAGCTGAAGATC CACATGCGGAAGCACACGGGAGAGCGCCCCTACTCATGCCCGCACTGCCCAGCCCGCTTCCTGCACAGC TACGACCTCAAGAACCACATGCACCTGCACACAGGGGACCGGCCCTATGAGTGCCACCTGTGCCACAAG GCTTTCGCCAAGGAGGACCACCTGCAGCGCCACCTCAAAGGCCAGAACTGCCTGGAGGTGCGCACCCGA CGGCGCCGCAAGGACGATGCACCACCCCACTACCCACCACCCTCTACCGCTGCTGCATCCCCCGCTGGC CTCGACCTCTCCAATGGCCACCTGGACACCTTCCGCCTCTCTCTAGCTCGATTCTGGGAGCAGTCAGCC CCCACTGGGCCCCCGGTCTCTACCCCAGGGCCCCCTGATGACGATGAGGAGGAAGGGGCACCCACCACA CCCCAGGCTGAAGGTGCCATGGAGTCCTCTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001256455
Insert Size	1620 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001256455.1
RefSeq Size	3790 bp
RefSeq ORF	1620 bp
Locus ID	51043
UniProt ID	O15156
Protein Families	Transcription Factors
MW	58 kDa
Gene Summary	This gene encodes a zinc finger-containing transcription factor that acts as a key regulator of lineage commitment of immature T-cell precursors. It is necessary and sufficient for commitment of CD4 lineage, while its absence causes CD8 commitment. It also functions as a transcriptional repressor of type I collagen genes. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2012] Transcript Variant: This variant (5) lacks two alternate exons in the 5' UTR and 5' coding region, and uses a downstream in-frame start codon, comapred to variant 3. The encoded isoform (1) is shorter at the N-terminus, compared to isoform 2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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